Results 101 to 110 of about 147,157 (316)

Engineered crRNA Drives RPA‐T7‐CRISPR/Cas14a Cascade for Ultrasensitive Detection of ctDNA PIK3CA H1047R

open access: yesAdvanced Science, EarlyView.
The TIDE‐Cas14a system, an integrated RPA‐T7‐CRISPR/Cas14a cascade platform incorporating engineered crRNA mismatches, enables ultrasensitive detection of the PIK3CA H1047R mutation in breast cancer circulating tumor DNA, with a limit of detection as low as 0.01%, surpassing the sensitivity of droplet digital PCR.
Yuanyuan Yu   +19 more
wiley   +1 more source

Epidemiology of Cutaneous Leishmaniasis Outbreak, Waziristan, Pakistan

open access: yesEmerging Infectious Diseases, 2018
During 2013–2015, prevalence of cutaneous leishmaniasis in war-affected Waziristan areas was 3.61% by PCR. Youths (1–15 years of age) were more susceptible.
Mubashir Hussain   +9 more
doaj   +1 more source

Top‐Down Fabricated Wood‐Derived Pressure and Strain Sensors: A Review

open access: yesAdvanced Science, EarlyView.
This review focuses on wood‐derived pressure/strain sensors fabricated via top‐down strategies. It analyzes wood's structural composition, examines processing techniques, discusses sensor types and sensing mechanisms, and reviews existing research. The article concludes with future directions for enhancing performance and scalability.
Yi Ren   +8 more
wiley   +1 more source

A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species

open access: yesCurrent Medical Mycology, 2017
Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for ...
Zahra Jafari   +5 more
doaj  

Possibilities of using nested PCR-RFLP for taxonomic identification of L3 larvae of the family Trichostrongylidae, Leiper, 1912

open access: yesРоссийский паразитологический журнал
The purpose of the research is to apply molecular genetic research methods to identify the taxonomic affiliation of gastrointestinal parasitic sheep nematodes of the family Trichostrongylidae using nested PCR followed by the restriction fragment length ...
I. A. Pimenov   +3 more
doaj   +1 more source

Molecular Differentiation of Mycobacterium tuberculosis Strains without IS6110 Insertions

open access: yesEmerging Infectious Diseases, 2002
By using standard restriction fragment length polymorphism, 6 zero-copy IS6110 Mycobacterium tuberculosis isolates were identified from 1,180 Maryland isolates as part of the National Tuberculosis Genotyping Surveillance Network Project. By using various
Kerry H. Lok   +8 more
doaj   +1 more source

Dna fingerprinting - review paper [PDF]

open access: yes, 2006
Before the Polymerase Chain Reaction (PCR) was established, DNA fingerprinting technology has relied for years on Restriction Fragment Length Polymorphism (RFLP) and Variable Number of Tandom Repeats (VNTR) analysis, a very efficient technique but ...
Blundell, Renald
core  

Inheritance and restriction fragment length polymorphism of chloroplast DNA in the genus Coffea L. [PDF]

open access: yes, 1996
CpDNA variation among 52 tree samples belonging to 25 different taxa of #Coffea$ and two species of #Psilanthus$ was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region.
Anthony, François   +6 more
core   +1 more source

IPGCA: A Comprehensive Single Cell Atlas of 1 074 127 Porcine Intestinal Cells Revealing Cellular Dynamics, Genetic Regulation, and Cross‐Species Conservation

open access: yesAdvanced Science, EarlyView.
A high resolution integrated cell atlas of the pig intestine provides insights into the genetic mechanisms of complex traits (Created in BioRender. Yu, P. (2025) https://BioRender.com/o14c563) Abstract The porcine intestinal tract is vital for nutrient absorption, immune regulation, and various physiological processes.
Pengfei Yu   +14 more
wiley   +1 more source

Análisis de polimorfismos en los genes tripanotión reductasa y cruzipaína en cepas colombianas de Trypanosoma cruzi

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2007
Introducción. Los estudios genéticos en Trypanosoma cruzi han buscado establecer asociaciones de variantes genéticas del parásito con manifestaciones clínicas de la enfermedad, origen biológico y geográfico de los aislamientos; sin embargo, los ...
Winston Rojas   +5 more
doaj   +1 more source

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