Results 21 to 30 of about 6,098 (217)
Optimization of Nonisotopic PCR–Single-Strand Conformation Polymorphism Analysis [PDF]
Clinical Chemistry, 1997 PCR–single-strand conformation polymorphism (SSCP) analysis is an attractive technique used to screen for unknown mutations because of its simplicity and widespread applicability (1). The technique relies on single-nucleotide variations modifying the conformation of single-stranded DNA and therefore its mobility in polyacrylamide gels. The detection of
H, Blanché +2 more
openaire +2 more sources
Mutational analysis of the PTPN11 gene in Egyptian patients with Noonan syndrome
Journal of the Formosan Medical Association, 2013 Noonan syndrome (NS) is inherited as an autosomal dominant disorder with dysmorphic facies, short stature, and cardiac defects, which can be caused by missense mutations in the protein tyrosine phosphatase nonreceptor type 11 (PTPN11) gene, which encodes
Mona L. Essawi +5 more
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BioTechniques, 2002 PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products.
Koichiro Higasa +3 more
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Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
Genetics and Molecular Biology, 2002 A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP).
Patricia M. Mirol +2 more
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Animals, 2023 The Cashmere goat is an excellent local goat breed in Gansu Province of China, and it is expected to improve cashmere production and cashmere quality through selection and breeding to enhance its commercial value.
Zhanzhao Chen +7 more
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Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives
Advanced Materials, EarlyView.Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi +4 more
wiley +1 more source
Investigation of Mutations in Exons 15 and 18 of MYBPC3 Gene in Hypertrophic Cardiomyopathy Patients [PDF]
Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk, 2015 Background: Hypertrophic cardiomyopathy (HCM) is a various collection of heart diseases with autosomal dominant inheritance affecting 0.2% of the global population.
Behnaz Sadat Abedi +4 more
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DNA Methylation: A Profile of Methods and Applications
BioTechniques, 2002 Ever since methylcytosine was found in genomic DNA, this epigenetic alteration has become a center of scientific attraction, especially because of its relation to gene silencing in disease.
Mario F. Fraga, Manel Esteller
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Implication of alterations in Parkin gene among North Indian patients with colorectal cancer
The Turkish Journal of Gastroenterology, 2020 Background/Aims: Alterations in Parkin (PRKN) have been described in many cancers; however, the molecular mechanism that contributes to loss of Parkin expression in colorectal cancer (CRC) remains unclear.
Raj Ranjan Tiwari +6 more
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Advanced Science, EarlyView.This study reveals the genetic and molecular mechanisms controlling grain size homeostasis through fine‐tuning OsGRX8 self‐expression by two natural negative feedback loops functioning in redox‐dependent or ‐independent manners and identifies two self‐regulatory haplotypes (SRHs) for the subspecies differentiation in rice.
Xingxing Li +13 more
wiley +1 more source