The effect of antibiotics on the T4polynucleotide ligase catalyzed template dependent polymerization of oligodeoxythymidylates [PDF]
The poly(dA) dependent T4 polynucleotide ligase catalyzed polymerization of oligodeoxythymidylates is dependent upon duplex stability. The antibiotics ethidium bromide, netropsin and Hoechst 33258 stabilize the duplex poly(dA) . P(dT)n (n = 6-10) to thermal denaturation.
Bernd W. Kalisch, Johan H. van de Sande
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Polynucleotide ligase from myeloid and lymphoid tissues. [PDF]
Tomas Lindahl, Gerald M. Edelman
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Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity. [PDF]
An RNA ligase that catalyzes the formation of a 2'-phosphomonoester-3',5'-phosphodiester bond in the presence of ATP and Mg2+ was purified approximately 6000-fold from raw wheat germ. A 5'-hydroxyl polynucleotide kinase activity copurified with RNA ligase through all chromatographic steps.
Leslie Pick, J Hurwitz
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A polynucleotide ligase from polyoma virus-infected mouse cells [PDF]
P Beard, J. D. Pitts
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Direct adenylation from 5'-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase. [PDF]
Yang Z+10 more
europepmc +2 more sources
Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD+-dependent polynucleotide ligases. [PDF]
Unciuleac MC, Goldgur Y, Shuman S.
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Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay
Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons+1 more
doaj +1 more source
Studies on the Joining of DNA by Polynucleotide Ligase of Phage T4
C C Richardson+5 more
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Dependence of the nature of the Holstein polaron motion in a polynucleotide chain subjected to a constant electric field on the initial polaron state and the parameters of the chain [PDF]
In this work, we consider the motion of a polaron in a polynucleotide Holstein molecular chain in a constant electric field. It is shown that the character of the polaron motion in the chain depends not only on the chosen parameters of the chain, but also on the initial distribution of the charge along the chain.
arxiv +1 more source
An onset model of mutually catalytic self-replicative systems formed by an assembly of polynucleotides [PDF]
Self-replicability is the unique attribute observed in all the living organisms and the question how the life was physically initiated could be equivalent to the question how self-replicating informative polymers were formed in the abiotic material world.
arxiv +1 more source