Results 11 to 20 of about 88,763 (155)

Polynucleotide 3′-terminal Phosphate Modifications by RNA and DNA Ligases [PDF]

Journal of Biological Chemistry, 2014
RNA and DNA ligases catalyze the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl ends of nucleic acids. In this work, we describe the ability of the thermophilic RNA ligase MthRnl from Methanobacterium thermoautotrophicum to recognize and modify the 3'-terminal phosphate of RNA and single-stranded DNA (ssDNA).
Alexander M, Zhelkovsky, Larry A, McReynolds   +1 more
openaire   +3 more sources

Interdomain assembly between the fungal tRNA ligase adenylyltransferase and kinase domain. [PDF]

RNA
Trl1-type ligases play an essential role in fungi and plants during the nonconventional tRNA splicing as well as the unfolded protein response. The tripartite enzyme consists of an N-terminal adenylyltransferase domain (LIG), a central polynucleotide ...
Köhler S, Peschek J.
europepmc   +2 more sources

Kinetic and structural insights into the requirement of fungal tRNA ligase for a 2'-phosphate end. [PDF]

RNA
Fungal RNA ligase (LIG) is an essential tRNA splicing enzyme that joins 3′-OH,2′-PO4 and 5′-PO4 RNA ends to form a 2′-PO4,3′-5′ phosphodiester splice junction. Sealing entails three divalent cation-dependent adenylate transfer steps.
Ghosh S, Shuman S.
europepmc   +2 more sources

Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD⁺-dependent polynucleotide ligases. [PDF]

Proc Natl Acad Sci U S A, 2017
Unciuleac MC, Goldgur Y, Shuman S.
europepmc   +2 more sources

Two distinct polynucleotide ligases from rat liver [PDF]

FEBS Letters, 1975
Teraoka, Hirobumi, Shimoyachi, Mari, Tsukada, Kinji   +2 more
openaire   +3 more sources

Covalent catalysis in nucleotidyl transfer reactions: essential motifs in Saccharomyces cerevisiae RNA capping enzyme are conserved in Schizosaccharomyces pombe and viral capping enzymes and among polynucleotide ligases. [PDF]

Proc Natl Acad Sci U S A, 1994
Shuman S, Liu Y, Schwer B.
europepmc   +2 more sources

Evolved DNAzymes and Stable Activation Chemistry Enable High-Efficiency DNA Ligation. [PDF]

Chemistry
In vitro selection of pre‐structured DNA libraries and chemical screening of alternative activation groups yielded trans‐acting DNAzymes with faster ligation rates and enhanced DNA substrate stability. The combination of C3S1 DNAzymes and phosphobenzimidazole‐activated DNA substrates provides improved DNA ligation performance, offering promise for ...
Nurmi C, Mendonsa G, Bao M, Li Y.
europepmc   +2 more sources

POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION [PDF]

Genetics, 1972
ABSTRACT Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period.
H M, Krisch, N V, Hamlett, H, Berger
openaire   +2 more sources

Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase [PDF]

Proceedings of the National Academy of Sciences, 1970
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends.
V, Sgaramella, J H, Van de Sande, H G, Khorana   +2 more
openaire   +2 more sources

Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay

Frontiers in Genetics, 2014
Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons, Catherine Marie Nickson   +1 more
doaj   +1 more source