Results 21 to 30 of about 88,763 (155)
Characterization of a Baculovirus Enzyme with RNA Ligase, Polynucleotide 5′-Kinase, and Polynucleotide 3′-Phosphatase Activities [PDF]
Journal of Biological Chemistry, 2004 The end-healing and end-sealing steps of the phage T4-induced RNA restriction-repair pathway are performed by two separate enzymes, a bifunctional polynucleotide 5'-kinase/3'-phosphatase and an ATP-dependent RNA ligase. Here we show that a single trifunctional baculovirus enzyme, RNA ligase 1 (Rnl1), catalyzes the identical set of RNA repair reactions.
Alexandra, Martins, Stewart, Shuman
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Polynucleotide Ligase Activity of Eukaryotic Topoisomerase I [PDF]
Molecular Cell, 1998 Introduction of a single ribonucleoside immediately 5' of the scissile phosphate of a duplex DNA substrate converts eukaryotic topoisomerase I into an endoribonuclease. Here, I demonstrate that the RNase reaction is reversible. Vaccinia topoisomerase can ligate 2', 3'-cyclic phosphate and 5'-hydroxyl termini annealed to a bridging template strand ...
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RNA: A publication of the RNA Society, 2016 Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that repairs RNA breaks with 2′,3′-cyclic-PO4 and 5′-OH ends. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to ...
B. S. Remus, B. Schwer, S. Shuman
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Distinctive kinetics and substrate specificities of plant and fungal tRNA ligases
RNA: A publication of the RNA Society, 2014 This paper presents detailed kinetic assays for three activities, cyclic phosphodiesterase, polynucleotide kinase, and ligase, of the trifunctional plant tRNA ligase AtRNL.
B. S. Remus, S. Shuman
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Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair
Nucleic Acids Research, 2011 Mutations in mitochondrial DNA (mtDNA) are implicated in a broad range of human diseases and in aging. Compared to nuclear DNA, mtDNA is more highly exposed to oxidative damage due to its proximity to the respiratory chain and the lack of protection ...
Nasser Tahbaz, Sudip Subedi, M. Weinfeld
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DNA Ligases: Progress and Prospects*
Journal of Biological Chemistry, 2009 DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates.
S. Shuman
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A Mutant of Bacteriophage T7 Deficient in Polynucleotide Ligase
Journal of Biological Chemistry, 1971 Abstract An amber mutant of bacteriophage T7, obtained by mutagenesis with hydroxylamine, fails to induce the T7 polynucleotide ligase upon infection of Escherichia coli. The mutant (T7amHA13) grows normally on a suppressor-negative host; its sensitivity to ultraviolet light is the same as that of wild type T7.
Y, Masamune +2 more
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Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures [PDF]
European Journal of Biochemistry, 1971 A polynucleotide ligase has been detected and purified over 300‐fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat‐resistant protein factor present in the crude extract.
S, Spadari, G, Ciarrocchi, A, Falaschi
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Kinetics and Effect of Salts and Polyamines on T4 Polynucleotide Ligase [PDF]
European Journal of Biochemistry, 1975 The kinetics of T4 polynucleotide ligase has been investigated at pH 8, 20 °C and using the double‐stranded DNA substrate (dA)n [(dT)10]n/10. Double‐reciprocal plots of initial rates vs substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to a ping‐pong mechanism. The overall mechanism was found
A J, Raae, R K, Kleppe, K, Kleppe
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Domain Structure and Mutational Analysis of T4 Polynucleotide Kinase*
Journal of Biological Chemistry, 2001 T4 polynucleotide kinase (Pnk) is the founding member of a family of 5′-kinase/3′-phosphatase enzymes that heal broken termini in RNA or DNA by converting 3′-PO4/5′-OH ends into 3′-OH/5′-PO4 ends, which are then suitable for sealing by RNA or DNA ligases.
L. Wang, S. Shuman
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