Results 21 to 30 of about 76,419 (179)
PARP (Poly ADP-ribose Polymerase) Family in Health and Disease. [PDF]
MedComm (2020)PARP family orchestrates ADP‐ribosylation to regulate genomic stability (DNA repair, telomeres) and metabolism (NAD⁺, glucose/lipid). Physiological roles sustain health; dysregulation drives pathologies: cancer (DNA repair addiction), neurodegeneration (parthanatos), cardiovascular disorders (hypertrophy/fibrosis), inflammation (dual role), and viral ...
Lei P +9 more
europepmc +2 more sources
Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay
Frontiers in Genetics, 2014 Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons +1 more
doaj +1 more source
Studies on the Joining of DNA by Polynucleotide Ligase of Phage T4
Cold Spring Harbor Symposia on Quantitative Biology, 1968 C C Richardson +5 more
openalex +4 more sources
RNA: A publication of the RNA Society, 2016 Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that repairs RNA breaks with 2′,3′-cyclic-PO4 and 5′-OH ends. Trl1 is composed of C-terminal cyclic phosphodiesterase and central polynucleotide kinase domains that heal the broken ends to ...
B. S. Remus, B. Schwer, S. Shuman
semanticscholar +1 more source
Distinctive kinetics and substrate specificities of plant and fungal tRNA ligases
RNA: A publication of the RNA Society, 2014 This paper presents detailed kinetic assays for three activities, cyclic phosphodiesterase, polynucleotide kinase, and ligase, of the trifunctional plant tRNA ligase AtRNL.
B. S. Remus, S. Shuman
semanticscholar +1 more source
Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair
Nucleic Acids Research, 2011 Mutations in mitochondrial DNA (mtDNA) are implicated in a broad range of human diseases and in aging. Compared to nuclear DNA, mtDNA is more highly exposed to oxidative damage due to its proximity to the respiratory chain and the lack of protection ...
Nasser Tahbaz, Sudip Subedi, M. Weinfeld
semanticscholar +1 more source
DNA Ligases: Progress and Prospects*
Journal of Biological Chemistry, 2009 DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates.
S. Shuman
semanticscholar +1 more source
Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures [PDF]
European Journal of Biochemistry, 1971 A polynucleotide ligase has been detected and purified over 300‐fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat‐resistant protein factor present in the crude extract.
Arturo Falaschi +2 more
openaire +3 more sources
Domain Structure and Mutational Analysis of T4 Polynucleotide Kinase*
Journal of Biological Chemistry, 2001 T4 polynucleotide kinase (Pnk) is the founding member of a family of 5′-kinase/3′-phosphatase enzymes that heal broken termini in RNA or DNA by converting 3′-PO4/5′-OH ends into 3′-OH/5′-PO4 ends, which are then suitable for sealing by RNA or DNA ligases.
L. Wang, S. Shuman
semanticscholar +1 more source
Nucleic Acids Research, 2020 Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3′-OH RNA strands in the context of 3′-OH/5′-PO4 nicked duplexes.
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar +1 more source