Results 31 to 40 of about 76,419 (179)

RtcB Is the RNA Ligase Component of an Escherichia coli RNA Repair Operon*

Journal of Biological Chemistry, 2011
RNA 2′,3′-cyclic phosphate ends play important roles in RNA metabolism as substrates for RNA ligases during tRNA restriction-repair and tRNA splicing.
N. Tanaka, S. Shuman
semanticscholar   +1 more source

Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems

ChemSystemsChem, EarlyView.
This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis, Joana Calvário, Inês Chibeles, Mohit Kumar, Ana S. Pina   +4 more
wiley   +1 more source

A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

Nucleic Acids Research, 2015
DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols.
G. Lohman, R. Bauer, N. Nichols, Laurie Mazzola, Joanna Bybee, Danielle Rivizzigno, Elizabeth Cantin, T. C. Evans   +7 more
semanticscholar   +1 more source

Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery

Molecular Ecology Resources, EarlyView.
ABSTRACT Ancient DNA (aDNA) analysis of archaeological dental calculus has provided a wealth of insights into ancient health, demography and lifestyles. However, the workflow for ancient metagenomics is still evolving, raising concerns about reproducibility.
Sterling L. Wright, Muslih Abdul‐Aziz, Grace N. Blaha, Christine K. Ta, Abigail Gancz, Iyunoluwa J. Ademola‐Popoola, Anna Szécsényi‐Nagy, Paul C. Sereno, Laura S. Weyrich   +8 more
wiley   +1 more source

Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase

Proceedings of the National Academy of Sciences of the United States of America, 2015
Significance Polynucleotide ligases are an ancient superfamily of nucleic acid repair enzymes that join 3′-OH and 5′-PO4 DNA or RNA ends. Ligases react with ATP or NAD+ to form a covalent enzyme–adenylate intermediate in which AMP is linked via a P–N ...
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar   +1 more source

The bZIP3‐AS1 complex promotes CONSTANS‐induced FLOWERING LOCUS T activation in a daylength‐dependent manner

Journal of Integrative Plant Biology, EarlyView.
The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee, Sun Ho Kim, Junsang Park, So Hee Yoon, Sung Won Cho, Nayoung Lee, Shogo Ito, Takato Imaizumi, Jong Chan Hong, Woo Sik Chung, Young Hun Song   +10 more
wiley   +1 more source

A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi

Molecular Microbiology, EarlyView.
We developed a resource set and protocols that allow researchers to quickly alter or silence genes in Group B Streptococcus. Using flexible plasmids and a drug‐inducible Cas12a system, the approach enables efficient generation of insertions, deletions, or knockdowns. These tools are publicly available.
G. H. Hillebrand, S. C. Carlin, E. J. Giacobe, H. A. Stephenson, J. Collins, T. A. Hooven   +5 more
wiley   +1 more source

Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit

Angewandte Chemie, Volume 137, Issue 29, July 14, 2025.
A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo, Aman Ishaqat, Junlin Chen, Fabian Wiertz, Adrian Kuzmanović, Matthias Bartneck, Fabian Kiessling, Andreas Herrmann   +7 more
wiley   +2 more sources

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, Volume 19, Issue 10, Page 2833-2859, October 2025.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

The Lrs14‐Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid‐Organizing Protein

MicrobiologyOpen, Volume 14, Issue 5, October 2025.
Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
Veerke De Kock, Ronnie Willaert, Yannick Gansemans, Filip Van Nieuwerburgh, Rani Baes, Eveline Peeters   +5 more
wiley   +1 more source