Results 31 to 40 of about 2,001 (190)

Solvation Effects on Hole Mobility in the Poly G/Poly C Duplex [PDF]

Russian Journal of Physical Chemistry A, 86, 832-836 (2012), 2013
Theoretical calculations of solvation contribution to hole energy in a polynucleotide chain give very low hole mobility values at zero temperature, \mu < 10^{-3} cm^2/(V s). We calculated hole mobility at physiological temperature for the Poly G/Poly C DNA duplex, which gave substantially larger mobility values.
arxiv   +1 more source

Construction of Reporter Phage T4::Nluc and Its Application in the Detection of Escherichia coli in Urinary Tract Infections

iLABMED, EarlyView.
A reporter phage T4::Nluc was constructed using the CRISPR/Cas9 system combined with homologous recombination and was applied for the detection of clinical Escherichia coli in urinary tract infections (UTIs). T4::Nluc exhibited superior sensitivity and specificity, and had a short detection time, making it a powerful tool for diagnosing UTIs.
Zhiyun Hao, Minwei Li, Qiang Zhao, Liye Wang, Ting Liu, Chi Wang, Chengbin Wang   +6 more
wiley   +1 more source

Current advances in the role of classical non‐homologous end joining in hematologic malignancies

Clinical and Translational Discovery, Volume 5, Issue 3, June 2025.
As depicted in the graphics, the classical NHEJ pathway can either take place in cells with DSBs or normal lymphocytes, reinforcing the stability of genome, and simultaneously maintaining the function of the immune system. While complete deletion causes immunodeficiency and tumourigenesis, some inhibitions could promote cancer therapies.
Pengcheng Liu, Zizhen Xu
wiley   +1 more source

Temperature dependence of circular DNA topological states [PDF]

PHYSICAL REVIEW E 79, 041926 (2009), 2009
Circular double stranded DNA has different topological states which are defined by their linking numbers. Equilibrium distribution of linking numbers can be obtained by closing a linear DNA into a circle by ligase. Using Monte Carlo simulation, we predict the temperature dependence of the linking number distribution of small circular DNAs.
arxiv   +1 more source

Defining short linear motif binding determinants by phage display‐based deep mutational scanning

Protein Science, Volume 34, Issue 6, June 2025.
Abstract Deep mutational scanning (DMS) has emerged as a powerful approach for evaluating the effects of mutations on binding or function. Here, we developed a DMS by phage display protocol to define the specificity determinants of short linear motifs (SLiMs) binding to peptide‐binding domains.
Caroline Benz, Lars Maassen, Leandro Simonetti, Filip Mihalic, Richard Lindqvist, Ifigenia Tsitsa, Aimiliani Konstantinou, Per Jemth, Anna K. Överby, Norman E. Davey, Ylva Ivarsson   +10 more
wiley   +1 more source

The m6A demethylase FTO promotes C/EBPβ‐LIP translation to perform oncogenic functions in breast cancer cells

The FEBS Journal, Volume 292, Issue 10, Page 2688-2709, May 2025.
N6‐methyladenosine is a key mRNA modification that regulates transcript turnover, translation, and other aspects of RNA fate. The m6A‐demethylase ‘eraser’ enzyme FTO reverses this modification. While FTO is known to promote oncogenic signaling in triple‐negative breast cancer, its wider role across the genome and specific downstream signaling has yet ...
Hidde R. Zuidhof, Christine Müller, Gertrud Kortman, René Wardenaar, Ekaterina Stepanova, Fabricio Loayza‐Puch, Cornelis F. Calkhoven   +6 more
wiley   +1 more source

Mg2+ ion effect on stability of double-stranded popynucleotide formed by polyriboinosinic and polyribocytidilic chains [PDF]

Biophysical Bulletin, 2, 68, 2005 (in russian), 2020
Mg2+ effect on the thermal stability of the double-stranded polynucleotide polyIpolyC (IC) under conditions close to physiological ones (0.1M Na+, pH7) was studied by the differential UV spectroscopy. The initial increase of the ion concentration ([Mg2+]) leads to the increase of the melting temperature (Tm) and to the decrease of the absorption ...
arxiv  

Complex Formation Between Polyelectrolytes and Ionic Surfactants [PDF]

, 1998
The interaction between polyelectrolyte and ionic surfactant is of great importance in different areas of chemistry and biology. In this paper we present a theory of polyelectrolyte ionic-surfactant solutions. The new theory successfully explains the cooperative transition observed experimentally, in which the condensed counterions are replaced by ...
arxiv   +1 more source

Randomness and optimality in enhanced DNA ligation with crowding effects [PDF]

Phys. Rev. Research 2, 013360 (2020), 2019
Enzymatic ligation is essential for the synthesis of long DNA. However, the number of ligated products exponentially decays as the DNA synthesis proceeds in a random manner. The controlling of ligation randomness is of importance to suppress exponential decay and demonstrate an efficient synthesis of long DNA. Here, we report the analysis of randomness
arxiv   +1 more source

Insights into the renal pathophysiology in Hermansky‐Pudlak syndrome‐1 from urinary extracellular vesicle proteomics and a new mouse model

FEBS Letters, Volume 599, Issue 7, Page 1055-1074, April 2025.
Hermansky‐Pudlak syndrome type 1 (HPS‐1) is a rare, autosomal recessive disorder with poorly understood renal involvement. Urinary extracellular vesicle (uEV) proteomics and a novel Hps1 mouse model reveal mitochondrial abnormalities and lipid accumulation in HPS‐1 kidney proximal tubule cells. Serum ApoA1 correlates with kidney function in our patient
Dawn M. Maynard, Bernadette R. Gochuico, Hadass Pri Chen, Christopher K. E. Bleck, Patricia M. Zerfas, Wendy J. Introne, William A. Gahl, May C. V. Malicdan   +7 more
wiley   +1 more source