Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Contribution of DNA repair and cell cycle checkpoint arrest to the maintenance of genomic stability [PDF]
, 2006 DNA damage response mechanisms encompass pathways of DNA repair, cell cycle checkpoint arrest and apoptosis. Together, these mechanisms function to maintain genomic stability in the face of exogenous and endogenous DNA damage.
Ahnesorg +41 more
core +1 more source
Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures [PDF]
European Journal of Biochemistry, 1971 A polynucleotide ligase has been detected and purified over 300‐fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat‐resistant protein factor present in the crude extract.
S, Spadari, G, Ciarrocchi, A, Falaschi
openaire +2 more sources
Phaeocystis globosa Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair [PDF]
, 2011 Phaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER).
Fernández-García, J.L. +3 more
core +4 more sources
DNA DSB repair pathway choice: an orchestrated handover mechanism [PDF]
, 2014 DNA double strand breaks (DSBs) are potential lethal lesions but can also lead to chromosome rearrangements, a step promoting carcinogenesis. DNA non-homologous end-joining (NHEJ) is the major DSB rejoining process and occurs in all cell cycle stages ...
A Kakarougkas +5 more
core +1 more source
Some observations on the cofactor requirement for partially purified DNA ligase from Escherichia coli [PDF]
, 1972 Farrando Sicilia, Jordi; Fuente Fuente ...
Richardson +10 more
core +1 more source
Efficient assembly of very short oligonucleotides using T4 DNA Ligase [PDF]
, 2010 Background In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short ...
Holt Robert A +2 more
core +2 more sources
Nanozymes Integrated Biochips Toward Smart Detection System
Advanced Science, EarlyView.This review systematically outlines the integration of nanozymes, biochips, and artificial intelligence (AI) for intelligent biosensing. It details how their convergence enhances signal amplification, enables portable detection, and improves data interpretation.
Dongyu Chen +10 more
wiley +1 more source
5‐Methylcytosine Analysis of miRNAs in Minimal Change Disease
Biotechnology and Applied Biochemistry, EarlyView.ABSTRACT Minimal change disease (MCD) is a glomerular disorder, which is the most common cause of nephrotic syndrome in children. Additionally, the prevalence of MCD in adults has been increasing in recent years. During protein synthesis, noncoding RNAs can be regulated through a variety of modifications, which helps preserve biological diversity and ...
Huiyi Zeng +14 more
wiley +1 more source
The role of the mammalian DNA end-processing enzyme polynucleotide kinase 3'-phosphatase in spinocerebellar ataxia Type 3 pathogenesis [PDF]
, 2015 DNA strand-breaks (SBs) with non-ligatable ends are generated by ionizing radiation, oxidative stress, various chemotherapeutic agents, and also as base excision repair (BER) intermediates.
A Das +78 more
core +4 more sources