, 2022 筑波大学University of Tsukuba博士（人間生物学）Doctor of Philosophy in Human Biology2022doctoral ...
Liu, Yancheng +2 more
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Contribution of DNA repair and cell cycle checkpoint arrest to the maintenance of genomic stability [PDF]
, 2006 DNA damage response mechanisms encompass pathways of DNA repair, cell cycle checkpoint arrest and apoptosis. Together, these mechanisms function to maintain genomic stability in the face of exogenous and endogenous DNA damage.
Ahnesorg +41 more
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Purification and Properties of a Polynucleotide Ligase from Human Cell Cultures [PDF]
European Journal of Biochemistry, 1971 A polynucleotide ligase has been detected and purified over 300‐fold from human cells cultured in vitro. The enzymic activity is dependent on ATP and Mg2+. The pH curve is bimodal with two optima at pH 7.5 and 8.1. The purified enzyme requires for its activity a heat‐resistant protein factor present in the crude extract.
S, Spadari, G, Ciarrocchi, A, Falaschi
openaire +2 more sources
Phaeocystis globosa Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair [PDF]
, 2011 Phaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER).
Fernández-García, J.L. +3 more
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Efficient assembly of very short oligonucleotides using T4 DNA Ligase [PDF]
, 2010 Background In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short ...
Holt Robert A +2 more
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Some observations on the cofactor requirement for partially purified DNA ligase from Escherichia coli [PDF]
, 1972 Farrando Sicilia, Jordi; Fuente Fuente ...
Richardson +10 more
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5‐Methylcytosine Analysis of miRNAs in Minimal Change Disease
Biotechnology and Applied Biochemistry, EarlyView.ABSTRACT Minimal change disease (MCD) is a glomerular disorder, which is the most common cause of nephrotic syndrome in children. Additionally, the prevalence of MCD in adults has been increasing in recent years. During protein synthesis, noncoding RNAs can be regulated through a variety of modifications, which helps preserve biological diversity and ...
Huiyi Zeng +14 more
wiley +1 more source
Evolved DNAzymes and Stable Activation Chemistry Enable High‐Efficiency DNA Ligation
Chemistry – A European Journal, EarlyView.In vitro selection of pre‐structured DNA libraries and chemical screening of alternative activation groups yielded trans‐acting DNAzymes with faster ligation rates and enhanced DNA substrate stability. The combination of C3S1 DNAzymes and phosphobenzimidazole‐activated DNA substrates provides improved DNA ligation performance, offering promise for ...
Connor Nurmi +3 more
wiley +1 more source
The role of the mammalian DNA end-processing enzyme polynucleotide kinase 3'-phosphatase in spinocerebellar ataxia Type 3 pathogenesis [PDF]
, 2015 DNA strand-breaks (SBs) with non-ligatable ends are generated by ionizing radiation, oxidative stress, various chemotherapeutic agents, and also as base excision repair (BER) intermediates.
A Das +78 more
core +4 more sources
The FEBS Journal, EarlyView.Redox environment modulates in vitro aggregation of Ataxin‐3, the protein implicated in spinocerebellar ataxia type 3. Reducing conditions stabilize native monomers and prevent aggregation, whereas oxidative conditions promote the formation of non‐native conformers and disulfide‐linked oligomers within the Josephin domain (JD).
Martyna Podlasiak +10 more
wiley +1 more source