Results 41 to 50 of about 76,419 (179)

Autosomal Recessive Cerebellar Ataxias: Translating Genes to Therapies

Annals of Neurology, Volume 98, Issue 3, Page 448-470, September 2025.
[Color figure can be viewed at www.annalsofneurology.org] Autosomal recessive cerebellar ataxias are disabling neurodegenerative genetic conditions affecting balance and coordination. Advancements in genomic testing have improved diagnosis, leading to a new focus on the development of targeted precision therapeutics addressing cellular, biochemical ...
Brent L. Fogel, Thomas Klopstock, David R. Lynch, Francesca Maltecca, Mayank Verma, Berge A. Minassian, Frances M. Platt, Débora Farina Gonçalves, Hélène Puccio, Andreas Roos, Matthis Synofzik   +10 more
wiley   +1 more source

Structure-Function Analysis of T4 RNA Ligase 2*

Journal of Biological Chemistry, 2003
Bacteriophage T4 RNA ligase 2 (Rnl2) exemplifies a polynucleotide ligase family that includes the trypanosome RNA-editing ligases and putative RNA ligases encoded by eukaryotic viruses and archaea.
Shenmin Yin, C. Ho, S. Shuman
semanticscholar   +1 more source

CRISPR/Cas9‐Mediated Gene Knockout in Cereal Crops

Current Protocols, Volume 5, Issue 9, September 2025.
Abstract High‐precision genome editing tools, such as programmable nucleases, are poised to transform crop breeding and significantly impact fundamental plant research. Among these tools, the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR‐associated 9) system is a programmable, RNA‐guided nuclease that introduces ...
Dibyajyoti Pramanik, Kan Wang, Keunsub Lee   +2 more
wiley   +1 more source

Deinococcus radiodurans HD-Pnk, a Nucleic Acid End-Healing Enzyme, Abets Resistance to Killing by Ionizing Radiation and Mitomycin C

Journal of Bacteriology, 2018
End healing is a process whereby nucleic acid breaks with “dirty” 3′-PO4 or 2′,3′-cyclic-PO4 and 5′-OH ends are converted to 3′-OH and 5′-PO4 termini that are amenable to downstream repair reactions.
B. J. Schmier, S. Shuman
semanticscholar   +1 more source

Pharmacological Targeting of DHHC9‐Mediated STRN4 Palmitoylation to Suppress YAP‐Driven Cancer Metastasis

Journal of Cellular and Molecular Medicine, Volume 29, Issue 17, September 2025.
ABSTRACT Protein S‐palmitoylation, a dynamic and reversible post‐translational modification involving the attachment of palmitate to cysteine residues, is a key regulator of protein functionality and cellular signalling. Dysregulation of this modification has emerged as a critical driver of cancer progression.
Yang Tian, Wei Li, Qing Zhai, Ying Yu, Jiaxin Yuan, Yan Ma, Jingjing Yang, Mingyue Li, Wenwen Chang, Wenjing Li, Keke Huang, Chongran Sun, Chen Zeng, Yingdi Sun, Jiabao Gu, Huilin Zhang, Dameng Li, Yanan Yu, Lu Hu, Peng Zhang, Bo Ma, Junnian Zheng, Pan Li, Feng Guo, Yang Sun   +24 more
wiley   +1 more source

A Two‐Component Pseudo‐Icosahedral Protein Nanocompartment with Variable Shell Composition and Irregular Tiling

Advanced Science, Volume 12, Issue 32, August 28, 2025.
The structure and assembly principles of a two‐component encapsulin nanocompartment shell are reported. The two‐component protein compartment self‐assembles from two distinct HK97‐fold shell proteins into a pseudo‐icosahedral protein shell with variable composition and irregular tiling.
Cassandra A. Dutcher, Michael P. Andreas, Tobias W. Giessen   +2 more
wiley   +1 more source

Photodynamic Therapy and Dietary Antioxidants: A Dual Strategy for Genome Stability and DNA Damage Repair

Cancer Medicine, Volume 14, Issue 15, August 2025.
ABSTRACT Background Photodynamic therapy (PDT) is an emerging cancer treatment that relies on photosensitizers (PS) activated by specific light wavelengths to produce reactive oxygen species (ROS), effectively targeting malignant cells. However, ROS can also harm surrounding healthy tissues, necessitating strategies to reduce unintended DNA damage ...
J. P. Jose Merlin, Sheeja S. Rajan, Heidi Abrahamse   +2 more
wiley   +1 more source

Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, Volume 5, Issue 4, August 2025.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang, Lianqing Wang, Chunbo Dong, Jialong Liu, Guoxin Cui, Shan Gao, Zhida Liu   +6 more
wiley   +1 more source

RNA 3′-Phosphate Cyclase (RtcA) Catalyzes Ligase-like Adenylylation of DNA and RNA 5′-Monophosphate Ends*

Journal of Biological Chemistry, 2010
RNA 3′-phosphate cyclase (Rtc) enzymes are a widely distributed family that catalyze the synthesis of RNA 2′,3′-cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of Rtc with ATP to form a covalent ...
A. Chakravarty, S. Shuman
semanticscholar   +1 more source

Structure-Function Analysis of the Phosphoesterase Component of the Nucleic Acid End-Healing Enzyme Runella slithyformis HD-Pnk

Journal of Bacteriology, 2019
5′-end healing and 3′-end healing are key steps in nucleic acid break repair in which 5′-OH ends are phosphorylated by a polynucleotide kinase, and 3′-PO4 or 2′,3′-cyclic-PO4 ends are hydrolyzed by a phosphoesterase to generate 5′-PO4 and 3′-OH termini ...
Annum Munir, S. Shuman
semanticscholar   +1 more source