Results 41 to 50 of about 3,900 (192)

Evolved DNAzymes and Stable Activation Chemistry Enable High‐Efficiency DNA Ligation

Chemistry – A European Journal, EarlyView.
In vitro selection of pre‐structured DNA libraries and chemical screening of alternative activation groups yielded trans‐acting DNAzymes with faster ligation rates and enhanced DNA substrate stability. The combination of C3S1 DNAzymes and phosphobenzimidazole‐activated DNA substrates provides improved DNA ligation performance, offering promise for ...
Connor Nurmi, Gemma Mendonsa, Mengdi Bao, Yingfu Li   +3 more
wiley   +1 more source

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts [PDF]

, 2016
Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states.
A Basters, A Kriz, AB Rode, AJ Jakobi, B Geering, B Weiss, BM Olivera, C Bieniossek, C Bieniossek, C Romier, C Schaffitzel, CP Chou, CV Robinson, D Busso, D Sari, D Schelshorn, DG Gibson, DJ Pitera, DN Gopaul, F Guo, F Leinert, G Bartani, G Gosset, G Kellenberger, H Alper, H Ye, HO Smith, J Komar, J Quan, J Torella, JA Chemler, JH Park, K Ballmer-Hofer, KB Mullis, LF Estrozi, LR Jarboe, LS Vijayachandran, M Folcher, M Gellert, M Haffke, M Haffke, M Meselson, MCY Chang, ML Diebold, ML Gefter, MZ Li, N Zheng, NR Cozzarelli, O Loeffelholz Von, PE Nisson, PJA Cock, R Patnaik, R Vincentelli, RJ Schulze, S Ausländer, S Cunna, S Linn, S Trowitzsch, S Trowitzsch, SC Almo, SE Luria, SJ Berríos-Rivera, SK Lee, SY Lee, T Maniatis, TC Terwilliger, VC Bode, W Abdulrahman, W Weber, WW Metcalf, Y Nie, Y Zhang   +71 more
core   +3 more sources

The Double Face of Base Excision Repair: Preventing and Triggering Double‐Strand Breaks

BioEssays, Volume 48, Issue 1, January 2026.
Base excision repair is conserved from bacteria to man, robustly eliminating oxidized bases in DNA and reinserting the appropriate nucleotide to restore an intact double helix. Recent results describe conditions and mechanisms that misregulate long‐patch BER to generate double‐strand breaks, triggering chromosome fragmentation and cell death, altering ...
Susan M. Gasser
wiley   +1 more source

Sister chromatid telomere fusions, but not NHEJ-mediated inter-chromosomal telomere fusions, occur independently of DNA ligases 3 and 4 [PDF]

, 2016
Telomeres shorten with each cell division and can ultimately become substrates for non-homologous end-joining repair, leading to large-scale genomic rearrangements of the kind frequently observed in human cancers. We have characterised over 1400 telomere
Ashelford, Kevin E., Baird, Duncan M., Harvey, Adam, Hendrickson, Eric A., Liddiard, Kate, Ruis, Brian, Takasugi, Taylor   +6 more
core   +2 more sources

Mycobacteriophage Mcgavigan Uses Noncanonical Bxb1‐Like Repressor for Heterotypic Superinfection Immunity

Journal of Basic Microbiology, Volume 66, Issue 1, January 2026.
ABSTRACT Mycobacteriophage Mcgavigan could be a promising candidate for use as a preventative agent against infections with Mycobacterium avium subsp. paratuberculosis. Bioinformatic analysis of the Mcgavigan genome revealed the presence of an operon containing a “Bxb1‐like” repressor.
Natali Shafer, Bradley E. Dubrule, Jeroen De Buck   +2 more
wiley   +1 more source

Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins [PDF]

, 2005
Pre-tRNA splicing is an essential process in all eukaryotes. It requires the concerted action of an endonuclease to remove the intron and a ligase for joining the resulting tRNA halves as studied best in the yeast Saccharomyces cerevisiae.
Beier, Hildburg, Englert, Markus
core   +2 more sources

Breast Cancer‐Secreted DPP3 Promotes Lung Metastasis by Remodelling the Vascular Niche in Lung via the Rap1 Signalling Pathway

Journal of Extracellular Vesicles, Volume 14, Issue 12, December 2025.
ABSTRACT Metastasis is the leading cause of death related to breast cancer. Premetastatic niches (PMNs), which are remodelled by the primary tumours in distant organs, are essential for the colonisation of disseminated cancer cells. The vascular niche is among the most pivotal PMNs in breast cancer lung metastasis, and the underlying mechanism remains ...
Xu Li, Sheng Hu, Ziqi Yuan, Xiaoyu Fu, Xiaohui Zhang, Liu Liu, Chaoqun Wang, Wei Yan, Juanjuan Li   +8 more
wiley   +1 more source

tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay. [PDF]

, 2019
Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that catalyzes exon-exon ligation during tRNA biogenesis and the non-conventional splicing of HAC1 mRNA during the unfolded protein response (UPR).
Peschek, Jirka, Walter, Peter
core  

An acidic patch in the unstructured N‐terminus modulates LSD1 activity

Protein Science, Volume 34, Issue 12, December 2025.
Abstract Lysine‐specific demethylase 1 (LSD1) plays a crucial role in chromatin organization and gene regulation by removing methyl groups from histone and non‐histone substrates. While its catalytic core is well characterized, the functional contributions of its intrinsically disordered N‐terminal region remain less understood.
Franziska Dukatz, Hermann Timofeev, Philipp Schnee, Philipp Rathert   +3 more
wiley   +1 more source

Structure–function analysis of the kinase-CPD domain of yeast tRNA ligase (Trl1) and requirements for complementation of tRNA splicing by a plant Trl1 homolog [PDF]

, 2006
Trl1 is an essential 827 amino acid enzyme that executes the end-healing and end-sealing steps of tRNA splicing in Saccharomyces cerevisiae.
Beier, Hildburg, Englert, Markus, Schwer, Beate, Shuman, Stewart, Wang, Li Kai   +4 more
core   +2 more sources