Results 41 to 50 of about 88,763 (155)
Journal of Basic Microbiology, Volume 66, Issue 1, January 2026.ABSTRACT Mycobacteriophage Mcgavigan could be a promising candidate for use as a preventative agent against infections with Mycobacterium avium subsp. paratuberculosis. Bioinformatic analysis of the Mcgavigan genome revealed the presence of an operon containing a “Bxb1‐like” repressor.
Natali Shafer +2 more
wiley +1 more source
RtcB Is the RNA Ligase Component of an Escherichia coli RNA Repair Operon*
Journal of Biological Chemistry, 2011 RNA 2′,3′-cyclic phosphate ends play important roles in RNA metabolism as substrates for RNA ligases during tRNA restriction-repair and tRNA splicing.
N. Tanaka, S. Shuman
semanticscholar +1 more source
HLA‐Knockout: Enabling Allele‐Specific Knockout of HLA Class I Genes for Immunogenic Engineering
HLA, Volume 107, Issue 1, January 2026.ABSTRACT The interaction between T‐cell receptors (TCRs) and antigenic peptides presented by HLA molecules is fundamental to adaptive immunity. However, the extreme polymorphism of HLA genes poses major challenges for transplantation, antigen discovery, immunotherapy and studies of allele‐specific function.
Connor Mattivi +4 more
wiley +1 more source
A high-throughput assay for the comprehensive profiling of DNA ligase fidelity
Nucleic Acids Research, 2015 DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols.
G. Lohman +7 more
semanticscholar +1 more source
Nucleic Acids Research, 2020 Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3′-OH RNA strands in the context of 3′-OH/5′-PO4 nicked duplexes.
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar +1 more source
Journal of Extracellular Vesicles, Volume 14, Issue 12, December 2025.ABSTRACT Metastasis is the leading cause of death related to breast cancer. Premetastatic niches (PMNs), which are remodelled by the primary tumours in distant organs, are essential for the colonisation of disseminated cancer cells. The vascular niche is among the most pivotal PMNs in breast cancer lung metastasis, and the underlying mechanism remains ...
Xu Li +8 more
wiley +1 more source
An acidic patch in the unstructured N‐terminus modulates LSD1 activity
Protein Science, Volume 34, Issue 12, December 2025.Abstract Lysine‐specific demethylase 1 (LSD1) plays a crucial role in chromatin organization and gene regulation by removing methyl groups from histone and non‐histone substrates. While its catalytic core is well characterized, the functional contributions of its intrinsically disordered N‐terminal region remain less understood.
Franziska Dukatz +3 more
wiley +1 more source
Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems
ChemSystemsChem, Volume 7, Issue 6, November 2025.This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis +4 more
wiley +1 more source
Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase
Proceedings of the National Academy of Sciences of the United States of America, 2015 Significance Polynucleotide ligases are an ancient superfamily of nucleic acid repair enzymes that join 3′-OH and 5′-PO4 DNA or RNA ends. Ligases react with ATP or NAD+ to form a covalent enzyme–adenylate intermediate in which AMP is linked via a P–N ...
M. Unciuleac, Y. Goldgur, S. Shuman
semanticscholar +1 more source
A CRISPRi Gene Regulation System for Bifidobacteria
Microbial Biotechnology, Volume 18, Issue 11, November 2025.We report the development of a single plasmid‐based CRISPR interference system based on a catalytically dead Cas9 from Streptococcus thermophilus for targeted gene repression in bifidobacteria. This system is capable of repressing genes in a diverse range of bifidobacteria enabling functional genomics studies in non‐model strains.
Lisa Friess +2 more
wiley +1 more source