Results 41 to 50 of about 2,001 (190)

Binding of Carminomycin to synthetic polyribonucleotides poly(A) and poly(U): absorption and polarized fluorescence study [PDF]

Biophysical Bulletin 488(1)(2000)56-59.(in Ukrainian), 2020
Binding of anthracycline antibiotic carminomycin (CM) to synthetic polyribonucleotides poly(A) and poly(U) was studied in solution of low ionic strength in a wide phosphate-to-dye (P/D) range using absorption and polarized fluorescence spectroscopy. Two different modes of CM binding to the ss-polynucleotides have been identified.
arxiv  

Complexation of DNA with Cationic Surfactant [PDF]

, 1999
Transfection of an anionic polynucleotide through a negatively charged membrane is an important problem in genetic engineering. The direct association of cationic surfactant to DNA decreases the effective negative charge of the nucleic acid, allowing the DNA-surfactant complex to approach a negatively charged membrane.
arxiv   +1 more source

A Novel Protein NLRP12‐119aa that Prevents Rhabdovirus Replication by Disrupting the RNP Complex Formation

Advanced Science, Volume 12, Issue 12, March 27, 2025.
The NLRP12‐119aa protein exerts its antiviral effect by two distinct mechanisms. First, it competes with the N protein of rhabdoviruses for binding to the viral leRNA, thereby preventing the formation of the RNP complex. Second, NLRP12‐119aa directly interacts with the rhabdovirus N protein, destabilizing it and promoting its degradation, which ...
Weiwei Zheng, Xiangxiang Zhu, Tongtong Zhu, Qiang Luo, Yan Zhao, Tianjun Xu   +5 more
wiley   +1 more source

Replication of proto-RNAs sustained by ligase-helicase cycle in oligomer world [PDF]

arXiv, 2013
A mechanism of the replication of proto-RNAs in oligomer world is proposed. The replication is carried out by a minimum cycle which is sustained by a ligase and a helicase. We expect that such a cycle actually worked in the primordial soup and can be constructed in vitro.
arxiv  

Characterization of Two Novel PNKP Splice‐Site Variants in a Proband With Microcephaly, Intellectual Disability, and Multiple Malformations

American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, Volume 198, Issue 2, March 2025.
ABSTRACT Polynucleotide kinase phosphatase (PNKP), encoded by the PNKP gene, is a DNA processing enzyme involved in double‐strand break and single‐strand break repair pathways, which are essential for genome stability and for the correct development and maintenance of human nervous system.
Ugo Sorrentino, Elisa Baschiera, Maria Andrea Desbats, Orsetta Zuffardi, Leonardo Salviati, Matteo Cassina   +5 more
wiley   +1 more source

Efficient Multiplex Genome Editing of the Cyanobacterium Synechocystis sp. PCC6803 via CRISPR‐Cas12a

Biotechnology and Bioengineering, Volume 122, Issue 3, Page 736-743, March 2025.
The authors have developed an optimised CRISPR‐Cas12a system for efficient genetic engineering in the polyploid cyanobacterium Synechocystis sp. PCC6803. Key advancements include modifying the RSF1010 plasmid to improve conjugation efficiency, optimizing guide RNA design for precise chromosomal cleavage, and utilizing sacB‐based counter‐selection to ...
Wei Du, Luna L. Meister, Tobias van Grinsven, Filipe Branco dos Santos   +3 more
wiley   +1 more source

70 MW-level picosecond mid-infrared radiation generation by difference frequency generation in AgGaS2, BaGa4Se7, LiGaSe2, and LiGaS2 [PDF]

IEEE Photonics Journal ( Volume: 17, Issue: 2, April 2025)
Comparative study of nonlinear crystals for picosecond difference frequency generation in mid-IR is presented. Nonlinear crystals of AgGaS$_2$, BaGa$_4$Se$_7$, LiGaSe$_2$, and LiGaS$_2$ were studied. Samples of AgGaS$_2$, BaGa$_4$Se$_7$, LiGaSe$_2$, and LiGaS$_2$ were tested in thee sets having lengths of 2, 4, or 8 mm.
arxiv   +1 more source

Targeting YBX1‐m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma

Clinical and Translational Medicine, Volume 15, Issue 3, March 2025.
NOP2/Sun RNA methyltransferase 2 (NSUN2) promotes 5‐methylcytosine (m5C) modification of Ring Finger Protein 115 (RNF115) mRNA, which is subsequently bound by YBX1. Then, YBX1 interacts with Eukaryotic Translation Initiation Factor 4A1 (EIF4A1) to promote RNF115 mRNA looping and translation.
Ouwen Li, Ke An, Han Wang, Xianbin Li, Yueqin Wang, Lan Huang, Yue Du, Nuo Qin, Jiasheng Dong, Jingyao Wei, Ranran Sun, Yong Shi, Yanjia Guo, Xiangyi Sun, Ying Yang, Yun‐Gui Yang, Quancheng Kan, Xin Tian   +17 more
wiley   +1 more source

Lonidamine, a Novel Modulator for the BvgAS System of Bordetella Species

Microbiology and Immunology, Volume 69, Issue 3, Page 133-147, March 2025.
ABSTRACT The Gram‐negative bacteria Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory diseases in various mammals. They share the BvgAS two‐component system, which regulates the phenotypic conversion between the virulent Bvg+ and avirulent Bvg– phases.
Natsuko Ota, Takashi Nishida, Daron M. Standley, Aalaa Alrahman Sherif, Satoshi Iwano, Dendi Krisna Nugraha, Toshiya Ueno, Yasuhiko Horiguchi   +7 more
wiley   +1 more source

Viral Evolution and Adaptation as a Multivariate Branching Process [PDF]

, 2011
In the present work we analyze the problem of adaptation and evolution of RNA virus populations, by defining the basic stochastic model as a multivariate branching process in close relation with the branching process advanced by Demetrius, Schuster and Sigmund ("Polynucleotide evolution and branching processes", Bull. Math. Biol. 46 (1985) 239-262), in
arxiv   +1 more source