Results 51 to 60 of about 88,763 (155)

Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery

Molecular Ecology Resources, Volume 25, Issue 8, November 2025.
ABSTRACT Ancient DNA (aDNA) analysis of archaeological dental calculus has provided a wealth of insights into ancient health, demography and lifestyles. However, the workflow for ancient metagenomics is still evolving, raising concerns about reproducibility.
Sterling L. Wright, Muslih Abdul‐Aziz, Grace N. Blaha, Christine K. Ta, Abigail Gancz, Iyunoluwa J. Ademola‐Popoola, Anna Szécsényi‐Nagy, Paul C. Sereno, Laura S. Weyrich   +8 more
wiley   +1 more source

Structure-Function Analysis of T4 RNA Ligase 2*

Journal of Biological Chemistry, 2003
Bacteriophage T4 RNA ligase 2 (Rnl2) exemplifies a polynucleotide ligase family that includes the trypanosome RNA-editing ligases and putative RNA ligases encoded by eukaryotic viruses and archaea.
Shenmin Yin, C. Ho, S. Shuman
semanticscholar   +1 more source

The bZIP3‐AS1 complex promotes CONSTANS‐induced FLOWERING LOCUS T activation in a daylength‐dependent manner

Journal of Integrative Plant Biology, Volume 67, Issue 11, Page 2982-2998, November 2025.
The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee, Sun Ho Kim, Junsang Park, So Hee Yoon, Sung Won Cho, Nayoung Lee, Shogo Ito, Takato Imaizumi, Jong Chan Hong, Woo Sik Chung, Young Hun Song   +10 more
wiley   +1 more source

A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi

Molecular Microbiology, Volume 124, Issue 5, Page 449-461, November 2025.
We developed a resource set and protocols that allow researchers to quickly alter or silence genes in Group B Streptococcus. Using flexible plasmids and a drug‐inducible Cas12a system, the approach enables efficient generation of insertions, deletions, or knockdowns. These tools are publicly available.
G. H. Hillebrand, S. C. Carlin, E. J. Giacobe, H. A. Stephenson, J. Collins, T. A. Hooven   +5 more
wiley   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, Volume 19, Issue 10, Page 2833-2859, October 2025.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

Polynucleotide Ligase Activity in Adenovirus-Infected Human Embryonic Kidney Cell Cultures

Experimental Biology and Medicine, 1970
SummaryCell-free extracts prepared from human embryonic kidney (HEK) cultures after infection with adenovirus 2 or 12 had a decreased ability to repair single-strand breaks in the HEK cell DNA substrate. The apparent loss of polynucleotide ligase activity found in infected cells may be related to the adenovirus-induced inhibition of HEK cell DNA ...
openaire   +2 more sources

Deinococcus radiodurans HD-Pnk, a Nucleic Acid End-Healing Enzyme, Abets Resistance to Killing by Ionizing Radiation and Mitomycin C

Journal of Bacteriology, 2018
End healing is a process whereby nucleic acid breaks with “dirty” 3′-PO4 or 2′,3′-cyclic-PO4 and 5′-OH ends are converted to 3′-OH and 5′-PO4 termini that are amenable to downstream repair reactions.
B. J. Schmier, S. Shuman
semanticscholar   +1 more source

Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity.

Journal of Biological Chemistry, 1986
An RNA ligase that catalyzes the formation of a 2'-phosphomonoester-3',5'-phosphodiester bond in the presence of ATP and Mg2+ was purified approximately 6000-fold from raw wheat germ. A 5'-hydroxyl polynucleotide kinase activity copurified with RNA ligase through all chromatographic steps.
L, Pick, J, Hurwitz
openaire   +2 more sources

Polynucleotide ligase from myeloid and lymphoid tissues. [PDF]

Proceedings of the National Academy of Sciences, 1968
T, Lindahl, G M, Edelman
openaire   +2 more sources

A polynucleotide ligase from polyoma virus-infected mouse cells [PDF]

Biochemical Journal, 1968
P, Beard, J D, Pitts
openaire   +2 more sources