Results 51 to 60 of about 2,001 (190)

mRNAs of plants and green algae lack the m7G cap‐1 structure

open access: yes
New Phytologist, Volume 246, Issue 2, Page 396-401, April 2025.
Chen Xiao   +7 more
wiley   +1 more source

A comparison and evaluation of strategies for bulk sequencing of mitogenomes from museum collections

open access: yesMethods in Ecology and Evolution, Volume 16, Issue 3, Page 530-545, March 2025.
Abstract Collecting mitochondrial genome sequences from museum collections has garnered increasing attention in recent years. Currently, several sequencing strategies have been proposed for this purpose, including direct DNA pooling, DNA labelling and mtDNA enrichment. It is important to understand the advantages and limitations of these methods before
Yao‐Wei Zhu   +4 more
wiley   +1 more source

Suppressed Protein Translation Caused by MSP‐8 Deficiency Determines Fungal Multidrug Resistance with Fitness Cost

open access: yesAdvanced Science, Volume 12, Issue 6, February 10, 2025.
This study identifies msp‐8 as a novel multidrug‐resistance gene encoding a helicase in fungi, and reveals a previously unrecognized resistance mechanism. Dysfunction of MSP‐8 disrupts translation homeostasis, leading to reductions in intracellular ketoconazolelevels, membrane‐bound amphotericin B contents, and reactive oxygen species.
Mi Zhou   +6 more
wiley   +1 more source

Nickel (II) Ions Interaction with Polynucleotides and DNA of Different GC Composition [PDF]

open access: yesarXiv, 2009
The goal of the work was to study the role of GC alternative dimmers in the binding of DNA with Ni (II) ions. The method of ultraviolet difference spectroscopy has been applied to investigate Ni (II) ions interactions with DNA extracted from Clostridium perfringens, Mice liver (C3HA line), Calf thymus, Salmon sperm, Herring sperm, E.coli, Micrococcus ...
arxiv  

The Mysterious World of Non‐Canonical Caps – What We Know and Why We Need New Sequencing Techniques

open access: yesChemBioChem, Volume 26, Issue 3, February 1, 2025.
This review describes sophisticated methodologies such as LC–MS that enabled the identification of 5’ non‐canonical RNA caps and also surveys so far developed sequencing methods, evaluating their advantages and disadvantages. It emphasizes the need for developing new, reliable sequencing techniques to identify RNA types with non‐canonical caps enabling
Flaminia Mancini, Hana Cahova
wiley   +1 more source

α‐N‐Methyltransferase regiospecificity is mediated by proximal, redundant enzyme–substrate interactions

open access: yesProtein Science, Volume 34, Issue 2, February 2025.
Abstract N‐Methylation of the peptide backbone confers pharmacologically beneficial characteristics to peptides that include greater membrane permeability and resistance to proteolytic degradation. The borosin family of ribosomally synthesized and post‐translationally modified peptides offer a post‐translational route to install amide backbone α‐N ...
Kathryn K. Crone   +3 more
wiley   +1 more source

The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum–Plasma Membrane Contact Sites and Interacts With AVRPM3b2/c2 Through Its LRR Domain

open access: yesMolecular Plant Pathology, Volume 26, Issue 2, February 2025.
PM3b co‐localises with S‐type but is excluded from V‐type endoplasmic reticulum–plasma membrane contact sites and is activated through a transient interaction with its corresponding powdery mildew avirulence effector, AVRPM3b2/c2. ABSTRACT Plant nucleotide‐binding leucine‐rich repeat (NLR) proteins are intracellular immune receptors that directly or ...
Jonatan Isaksson   +4 more
wiley   +1 more source

Enzymatic Joining of Polynucleotides

open access: yesJournal of Biological Chemistry, 1972
Abstract Incubation of superhelical closed circular DNA with Escherichia coli DNA ligase and adenosine 5'-monophosphate (AMP) yields two new circular forms: molecules with one single strand break and covalently closed circles that have lost superhelical turns.
I. R. Lehman   +2 more
openaire   +3 more sources

In VitroRepair of Single–strand Breaks in γ-irradiated DNA by Polynucleotide Ligase

open access: yesInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1972
SummaryThe ability of polynucleotide ligase to eliminate single-strand breaks in γ-irradiated DNA was studied. The irradiated DNA was first labelled at the 5′-end of the break with 32P-phosphate and treated in vitro with polynucleotide ligase. In phosphoester-bonds joined by the ligase, the incorporated 32P-phosphate is resistant to subsequent ...
Jacobs, A., Bopp, A., Hagen, U.
openaire   +4 more sources

Solvable models of neighbor-dependent nucleotide substitution processes [PDF]

open access: yesarXiv, 2005
We prove that a wide class of models of Markov neighbor-dependent substitution processes on the integer line is solvable. This class contains some models of nucleotide substitutions recently introduced and studied empirically by molecular biologists. We show that the polynucleotide frequencies at equilibrium solve explicit finite-size linear systems ...
arxiv  

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