Results 51 to 60 of about 3,904 (190)
Viroids: Survivors from the RNA World? [PDF]
, 2014 Because RNA can be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before DNA and proteins, which eventually assumed these roles and relegated RNA to intermediate functions.
Flores, Ricardo +3 more
core +1 more source
An acidic patch in the unstructured N‐terminus modulates LSD1 activity
Protein Science, Volume 34, Issue 12, December 2025.Abstract Lysine‐specific demethylase 1 (LSD1) plays a crucial role in chromatin organization and gene regulation by removing methyl groups from histone and non‐histone substrates. While its catalytic core is well characterized, the functional contributions of its intrinsically disordered N‐terminal region remain less understood.
Franziska Dukatz +3 more
wiley +1 more source
Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans [PDF]
, 2007 BACKGROUND: Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans genome after extremely high doses ...
Blasius, Melanie +3 more
core +4 more sources
Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems
ChemSystemsChem, Volume 7, Issue 6, November 2025.This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis +4 more
wiley +1 more source
A CRISPRi Gene Regulation System for Bifidobacteria
Microbial Biotechnology, Volume 18, Issue 11, November 2025.We report the development of a single plasmid‐based CRISPR interference system based on a catalytically dead Cas9 from Streptococcus thermophilus for targeted gene repression in bifidobacteria. This system is capable of repressing genes in a diverse range of bifidobacteria enabling functional genomics studies in non‐model strains.
Lisa Friess +2 more
wiley +1 more source
The 2'-5' RNA Ligase of Escherichia coli: Purification, Cloning, and Genomic Disruption [PDF]
, 1996 An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2-5 phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33,
Abelson, John N., Arn, Eric A.
core
Structure–function analysis of the kinase-CPD domain of yeast tRNA ligase (Trl1) and requirements for complementation of tRNA splicing by a plant Trl1 homolog [PDF]
, 2006 Trl1 is an essential 827 amino acid enzyme that executes the end-healing and end-sealing steps of tRNA splicing in Saccharomyces cerevisiae.
Beier, Hildburg +4 more
core +2 more sources
Molecular Ecology Resources, Volume 25, Issue 8, November 2025.ABSTRACT Ancient DNA (aDNA) analysis of archaeological dental calculus has provided a wealth of insights into ancient health, demography and lifestyles. However, the workflow for ancient metagenomics is still evolving, raising concerns about reproducibility.
Sterling L. Wright +8 more
wiley +1 more source
Journal of Integrative Plant Biology, Volume 67, Issue 11, Page 2982-2998, November 2025.The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee +10 more
wiley +1 more source
A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi
Molecular Microbiology, Volume 124, Issue 5, Page 449-461, November 2025.We developed a resource set and protocols that allow researchers to quickly alter or silence genes in Group B Streptococcus. Using flexible plasmids and a drug‐inducible Cas12a system, the approach enables efficient generation of insertions, deletions, or knockdowns. These tools are publicly available.
G. H. Hillebrand +5 more
wiley +1 more source