Results 31 to 40 of about 16,842 (191)

Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs

Bio-Protocol, 2018
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases.
Christian Beren, Katherine Liu, Lisa Dreesens, Charles Knobler, William Gelbart   +4 more
doaj   +1 more source

Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase

Journal of Biological Chemistry, 1975
The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y, Chou, M F, Singer, P, McPhie
openaire   +2 more sources

Lack of PNPase activity in Enterococcus faecalis 14 increases the stability of EntDD14 bacteriocin transcripts

Scientific Reports, 2023
A mutant deficient in polynucleotide phosphorylase (PNPase) activity was previously constructed in Enterococcus faecalis 14; a strain producing a leaderless two-peptide enterocin DD14 (EntDD14).
Rabia Ladjouzi, Anca Lucau-Danila, Paloma López, Djamel Drider   +3 more
doaj   +1 more source

Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties [PDF]

, 1982
Polynucleotide phosphorylase from cells of the cyanobacterium Synechococcus sp. has been purified 1400-fold by an improved procedure. The enzyme purified to homogeneity and lacking nuclease, phosphatase and protease contaminations reveals a single band ...
Nolden, W.-T., Richter, G.
core   +1 more source

Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase [PDF]

Nucleic Acids Research, 1976
It is already known that modification of E. coli polynucleotide phosphorylase by endogenous proteolysis induces drastic changes in both phosphorolysis and polymerisation reactions. The structural parameters of the proteolysed polynucleotide phosphorylase are described.
A, Guissani, C, Portier
openaire   +2 more sources

Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica

, 2002
For many pathogens, the ability to regulate their replication in host cells is a key element in establishing persistency. Here, we identified a single point mutation in the gene for polynucleotide phosphorylase (PNPase) as a factor affecting bacterial ...
Sofia Eriksson, Sacha Lucchini, Mikael Rhen, Arthur Thompson, Mark O. Clements, Normark, S.J., Eriksson, S., Lucchini, S., Hinton, J.C.D., Jay C. D. Hinton, Rhen, M., Staffan Normark, Clements, M.O., Thompson, A.   +13 more
core   +1 more source

RNase E biomolecular condensates stimulate PNPase activity

Scientific Reports, 2023
Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins, Dylan T. Tomares, Vidhyadhar Nandana, Jared M. Schrader, W. Seth Childers   +4 more
doaj   +1 more source

Genome wide association studies for acid phosphatase activity at varying phosphorous levels in Brassica juncea L

Frontiers in Plant Science, 2022
Acid phosphatases (Apases) are an important group of enzymes that hydrolyze soil and plant phosphoesters and anhydrides to release Pi (inorganic phosphate) for plant acquisition. Their activity is strongly correlated to the phosphorus use efficiency (PUE)
Priyanka Upadhyay, Mehak Gupta, Simarjeet Kaur Sra, Rakesh Sharda, Sanjula Sharma, Virender K. Sardana, Javed Akhatar, Gurpreet Kaur   +7 more
doaj   +1 more source

Opposing Contributions of Polynucleotide Phosphorylase and the Membrane Protein NlpI to Biofilm Formation by Salmonella enterica Serovar Typhimurium

, 2011
CsgD and cyclic-3′,5′-di-guanylate are key regulators of biofilm formation in Salmonella enterica serovar Typhimurium.
Vodnala, SK, Anwar, N,, Mikael Rhen, Romling, U, Ute Römling, Syed Fazle Rouf, Abdul Kader, Kader, A, Rhen, M,, Naeem Anwar, Rouf, SF, Irfan Ahmad, Ahmad, I,, Suman Kumar Vodnala   +13 more
core   +1 more source

Resveratrol Attenuates Exercise-Induced Adaptive Responses in Rats Selectively Bred for Low Running Performance

Dose-Response, 2014
Low capacity runner (LCR) rats have been developed by divergent artificial selection for treadmill endurance capacity to explore an aerobic biology-disease connection.
Nikolett Hart, Linda Sarga, Zsolt Csende, Lauren G. Koch, Steven L. Britton, Kelvin J.A. Davies, Zsolt Radak   +6 more
doaj   +1 more source