High‐Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates
Small, Volume 19, Issue 1, January 4, 2023., 2023 Positively charged zwitterionic coacervates are designed to purify extracellular vesicles in high yields. This system combines several advantages of different purification methods, including the scalability of precipitation, the gentle phase of aqueous two‐phase systems, and the programmability of chromatography. Abstract Programmable coacervates based
Carolina Paganini +8 more
wiley +1 more source
Biomedicines, 2020 Inflammatory bowel diseases (IBDs) are chronic conditions that can benefit from the combined treatment of adenosine receptor agonists and hyaluronic acid (HA), which, binding the CD44, has pro-survival effects.
Giovanni Pallio +9 more
doaj +1 more source
Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles
Nature Communications, 2022 Simple methods for attaching polynucleotides to gold nanoparticles are of interest for simplifying conjugation in a range of applications. Here, the authors report a microwave heating-based method for the fast, one-step attachment of a range of thiolated
Mengqi Huang +8 more
doaj +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Braeckmans, Kevin +2 more
core +2 more sources
Carbethoxylation of Polynucleotides [PDF]
European Journal of Biochemistry, 1971 Diethyl pyrocarbonate has been shown to react with poly(A), poly(C) and poly(G) but poly(U) does not react. Diethyl pyrocarbonate appears to carbethoxylate amino groups on the bases. Poly(A) can bind one carbethoxy group per base but the binding capacity of poly(G) and poly(C) under non‐denaturing conditions is 0.32 and 0.23, respectively.
openaire +3 more sources
Dry/Wet Cycling and the Thermodynamics and Kinetics of Prebiotic Polymer Synthesis
Life, 2016 The endoergic nature of protein and nucleic acid assembly in aqueous media presents two questions that are fundamental to the understanding of life’s origins: (i) how did the polymers arise in an aqueous prebiotic world; and (ii) once formed in some ...
David S. Ross, David Deamer
doaj +1 more source
A Raman spectroscopic study of the interaction between nucleotides and the DNA binding protein gp32 of bacteriophage T4 [PDF]
, 1987 Raman spectra of the bacteriophage T4 denaturing protein gp32, its complex with the polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA)8 and (dA)2, were recorded and interpreted.
Greve, J., Mul, F.F.M. de, Otto, C.
core +2 more sources
Dependence of the nature of the Holstein polaron motion in a polynucleotide chain subjected to a constant electric field on the initial polaron state and the parameters of the chain [PDF]
, 2021 In this work, we consider the motion of a polaron in a polynucleotide Holstein molecular chain in a constant electric field. It is shown that the character of the polaron motion in the chain depends not only on the chosen parameters of the chain, but also on the initial distribution of the charge along the chain.
arxiv +1 more source
Nucleic acid vibrational circular dichroism, absorption, and linear dichroism spectra. I. A DeVoe theory approach [PDF]
, 1997 Infrared (IR) vibrational circular dichroism (VCD), absorption, and linear dichroism (LD) spectra of four homopolyribonucleotides, poly(rA), poly(rG), poly(rC), and poly(rU), have been calculated, in the 1750–1550 cm-1 spectral region, using the DeVoe ...
Self, B.D., Moore, D.S.
core +1 more source
Nature Communications, 2023 Modern cells are complex chemical compartments tightly regulated by an underlying DNA-encoded program. Achieving a form of coupling between molecular content, chemical reactions, and chassis in synthetic compartments represents a key step to the assembly
Tommaso P. Fraccia, Nicolas Martin
doaj +1 more source