Results 31 to 40 of about 6,340 (193)
vAL‐1, a novel polysaccharide lyase encoded by chlorovirus CVK2 [PDF]
FEBS Letters, 2004 Cell wall materials isolated from Chlorella cells were degraded by the polysaccharide‐degrading enzyme vAL‐1 encoded by chlorovirus CVK2. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analyses of the degradation products (oligosaccharides) revealed major oligosaccharides contain unsaturated GlcA at the reducing terminus,
Ichiro Sugimoto +4 more
openaire +3 more sources
Exploring molecular determinants of polysaccharide lyase family 6–1 enzyme activity [PDF]
Glycobiology, 2021 Abstract The polysaccharide lyase family 6 (PL6) represents one of the 41 polysaccharide lyase families classified in the CAZy database with the vast majority of its members being alginate lyases grouped into three subfamilies, PL6_1–3. To decipher the mode of recognition and action of the enzymes belonging to subfamily PL6_1, we solved ...
Violot, Sébastien +9 more
openaire +4 more sources
Structural and mechanistic classification of uronic acid-containing polysaccharide lyases [PDF]
Glycobiology, 2010 Polysaccharide lyases (PLs) have been assigned to 21 families based on their sequences, with ~ 50 singletons awaiting further classification. For 19 of these families, the structure of at least one protein is known. In this review, we have analyzed the available structural information and show that presently known PL families belong to six general ...
Garron, Marie-Line, Cygler, Miroslaw
openaire +3 more sources
ACS Central Science, 2022 Dynamics is an essential process to drive an enzyme to perform a function. When a protein sequence encodes for its three-dimensional structure and hence its function, it essentially defines the intrinsic dynamics of the molecule. The static X-ray crystal structure was thought to shed little insight into the molecule's dynamics until the recently ...
Prerana Dash, Rudresh Acharya
openaire +2 more sources
Molecular Plant-Microbe Interactions, 2006 Two pectate lyase genes (Bx-pel-1 and Bx-pel-2) were cloned from the pine wood nematode, Bursaphelenchus xy-lophilus. The deduced amino acid sequences of these pectate lyases are most similar to polysaccharide lyase family 3 proteins.
Taisei Kikuchi +3 more
doaj +1 more source
A Novel Structural Fold in Polysaccharide Lyases [PDF]
Journal of Biological Chemistry, 2007 Rhamnogalacturonan (RG) lyase produced by plant pathogenic and saprophytic microbes plays an important role in degrading plant cell walls. An extracellular RG lyase YesW from saprophytic Bacillus subtilis is a member of polysaccharide lyase family 11 and cleaves glycoside bonds in polygalacturonan as well as RG type-I through a beta-elimination ...
Akihito Ochiai +6 more
openalex +5 more sources
Proceedings of the National Academy of SciencesAcidic glycans are essential for the biology of multicellular eukaryotes. To utilize them, microbial life including symbionts and pathogens has evolved polysaccharide lyases (PL) that cleave their 1,4 glycosidic linkages via a β-elimination mechanism. PL family 33 (PL33) enzymes have the unusual ability to target a diverse range of glycosaminoglycans ...
Mélanie Loiodice +18 more
openalex +6 more sources
Alginate Lyases: Sources, Mechanism of Activity and Potencial Application
Squalen, 2013 Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological ...
Subaryono Subaryono +3 more
doaj +1 more source
An engineered polysaccharide lyase to combat harmful algal blooms
Biochemical Engineering Journal, 2018 Abstract A growing global population and industrialization have come at the cost of induced climate change and pollution of natural resources, resulting in formation of toxic algal blooms in fresh water sources. In the US alone, these blooms cost an estimated $1.5 billion dollars each year to remediate.
Evan L. Eckersley, Bryan W. Berger
openalex +4 more sources
Marine Drugs, 2019 Alginate lyases degrade alginate into oligosaccharides, of which the biological activities have vital roles in various fields. Some alginate lyases contain one or more carbohydrate-binding modules (CBMs), which assist the function of the catalytic ...
Zhelun Zhang +5 more
doaj +1 more source