Microbiology Spectrum, 2023 The production of enterovirus virus-like particles (VLPs) that lack the viral genome have great potential as vaccines for a number of diseases, such as poliomyelitis and hand, foot, and mouth disease.
Lee Sherry +7 more
doaj +1 more source
Enhancement of heterologous gene expression in Flammulina velutipes using polycistronic vectors containing a viral 2A cleavage sequence. [PDF]
PLoS ONE, 2013 Agrobacterium tumefaciens-mediated transformation for edible mushrooms has been previously established. However, the enhancement of heterologous protein production and the expression of multi-target genes remains a challenge.
Yu-Ju Lin +2 more
doaj +1 more source
Death receptor-based enrichment of Cas9-expressing cells [PDF]
, 2016 Background: The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requires a high DNA editing efficiency.
Aschenbrenner, S. +6 more
core +1 more source
Detection and molecular characterization of porcine enterovirus G15 and teschovirus from India
Pathogens and Disease, 2020 ABSTRACT Porcine enterovirus G (EV-G) and teschovirus (PTV) generally cause asymptomatic infections. Although both viruses have been reported from various countries, they are rarely detected from India. To detect these viruses in Western India, fecal samples (n = 26) of diarrheic piglets aged below three months from private pig farms ...
Pradeep Mahadev Sawant +3 more
openaire +2 more sources
BMC Veterinary Research, 2017 Background The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology.
N. B. Goecke +12 more
doaj +1 more source
Sapovirus translation requires an interaction between VPg and the cap binding protein eIF4E. [PDF]
, 2014 UNLABELLED: Sapoviruses of the Caliciviridae family of small RNA viruses are emerging pathogens that cause gastroenteritis in humans and animals. Molecular studies on human sapovirus have been hampered due to the lack of a cell culture system.
Chaudhry, Yasmin +4 more
core +2 more sources
SPLICEIT Fluorescent Sensor for Integrating Dopamine Release with Cellular Resolution
Angewandte Chemie, Volume 137, Issue 33, August 11, 2025.“Single‐chain protein‐based ligand indication through a chimerically‐engineered integrator tool” (SPLICEIT) is a G‐protein coupled receptor‐based sensor design that leaves a permanent fluorescent mark. We show dopamine detection in primary cultured neurons with a fourfold signal increase normalized to an mCherry (mCh) marker.
Steven M. Havens +7 more
wiley +2 more sources
Epidemiologic investigation of immune-mediated polyradiculoneuropathy among abattoir workers exposed to porcine brain [PDF]
, 2003 Background In October 2007, a cluster of patients experiencing a novel polyradiculoneuropathy was identified at a pork abattoir (Plant A). Patients worked in the primary carcass processing area (warm room); the majority processed severed heads (head ...
Holzbauer, Stacy M. +21 more
core +2 more sources
Non‑ambulatory pigs in two Brazilian growing‑finishing farms : a clinic, etiological and pathological perspective on 76 cases [PDF]
, 2022 Background: Non-ambulatory pigs, colloquially known as downers or downed pigs, are animals presented with limited to no mobility, usually as a result of pre-existing neurologic or musculoskeletal conditions.
Almeida, Bruno Albuquerque de +8 more
core +1 more source
Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
Frontiers in Microbiology, 2020 Eukaryotic microorganisms use monocistronic mRNAs to encode proteins. For synthetic biological approaches like metabolic engineering, precise co-expression of several proteins in space and time is advantageous.
Kira Müntjes +7 more
doaj +1 more source