Results 141 to 150 of about 7,972 (181)
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Studies on the in vitro synthesis of ppGpp and pppGpp
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975The effect of a number of inhibitors of protein synthesis on ppGpp and pppGpp synthesis in vitro has been examined. As expected from in vivo results, chloramphenicol is without effect on this reaction. Aurintricarboxylic acid and chlortetracycline, on the other hand rapidly and specifically inhibit ppGpp synthesis.
L, Beres, J, Lucas-Lenard
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Anomalous synthesis of ppGpp in growing cells
Cell, 1977In E. coli cells, accumulation of ppGpp is normally triggered by conditions that restrict the aminoacylation of tRNA or interfere with carbon/energy source metabolism; in both cases, the nucleotide's accumulation is associated with control of stable RNA synthesis and is generally believed to bring it about.
J, Gallant, L, Palmer, C C, Pao
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ppGpp: a global regulator in Escherichia coli
Trends in Microbiology, 2005The small nucleotide ppGpp acts as a global regulator of gene expression in bacteria. Proteomic analysis of cells lacking ppGpp has shown that this nucleotide might affect many more genes than previously anticipated. These findings and others suggest that ppGpp causes a redirection of transcription so that genes important for starvation survival and ...
Lisa U, Magnusson +2 more
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Effect of ppGpp on the accuracy of protein biosynthesis
Archives of Biochemistry and Biophysics, 1983The maintenance of accuracy in protein biosynthesis in amino acid-starved rel+ strains of Escherichia coli has been attributed to an effect of ppGpp on the accuracy of aa-tRNA selection by the ribosome. It has been determined that concentrations of ppGpp characteristic of those found in amino acid-starved cells have no effect on the rate of reaction of
D B, Dix +3 more
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ppGpp: magic beyond RNA polymerase
Nature Reviews Microbiology, 2012During stress, bacteria undergo extensive physiological transformations, many of which are coordinated by ppGpp. Although ppGpp is best known for enhancing cellular resilience by redirecting the RNA polymerase (RNAP) to certain genes, it also acts as a signal in many other cellular processes in bacteria. After a brief overview of ppGpp biosynthesis and
Zachary D, Dalebroux, Michele S, Swanson
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The magic dance of the alarmones (p)ppGpp
Molecular Microbiology, 2016SummaryThe alarmones (p)ppGpp are important second messengers that orchestrate pleiotropic adaptations of bacteria and plant chloroplasts in response to starvation and stress. Here, we review our structural and mechanistic knowledge on (p)ppGpp metabolism including their synthesis, degradation and interconversion by a highly diverse set of enzymes ...
Wieland, Steinchen, Gert, Bange
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Synthesis of ppGpp by mouse embryonic ribosomes
Cell, 1974Abstract The unusual nucleotide guanosine tetraphosphate (ppGpp) is synthesized in vitro by ribosomes isolated from mouse embryos, but is not formed by ribosomes isolated from adults. Analysis of specific organs shows a developmental change in detectable ppGpp-forming ability.
J D, Irr, M S, Kaulenas, B R, Unsworth
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Modulation of RNA polymerase specificity by ppGpp
Molecular and General Genetics MGG, 1976ppGpp alters the initiation specificity of RNA polymerase holoenzyme in vitro in a direction which mimics the stringent response in vivo. The transition temperature for opening rRNA promoters is increased by the nucleotide, that for opening phi80 promoters is unaffected.
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1980
Faced with a shortage of nutrient source, bacteria rapidly adjust their metabolism and adapt to a more frugal life style. Of the doubtless many overlapping and hierarchical controls required for such an adjustment, a simple mechanism mediated by the peculiar nucleotide guanosine 5′-diphosphate,3′-diphosphate (ppGpp) will be reviewed and its metabolism ...
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Faced with a shortage of nutrient source, bacteria rapidly adjust their metabolism and adapt to a more frugal life style. Of the doubtless many overlapping and hierarchical controls required for such an adjustment, a simple mechanism mediated by the peculiar nucleotide guanosine 5′-diphosphate,3′-diphosphate (ppGpp) will be reviewed and its metabolism ...
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A gene involved in the metabolic control of ppGpp synthesis
Molecular and General Genetics MGG, 1978A genetic locus has been identified which controls the basal synthesis of ppGpp in growing E. coli. Cells carrying a recessive allele of the relX gene have a very low concentration of ppGpp during balanced growth, and fail to accumulate ppGpp in response to carbon/energy source downshift.
C C, Pao, J, Gallant
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