Rational Design of an Activatable Reporter for Quantitative Imaging of RNA Aberrant Splicing In Vivo
Molecular Therapy: Methods & Clinical Development, 2020 Pre-mRNA splicing, the process of removing introns from pre-mRNA and the arrangement of exons to produce mature transcripts, is a crucial step in the expression of most eukaryote genes. However, the splicing kinetics remain poorly characterized in living
Jinrong Xie +5 more
doaj
Interaction of hnRNPA1/A2 and DAZAP1 with an Alu-derived intronic splicing enhancer regulates ATM aberrant splicing. [PDF]
PLoS ONE, 2011 We have previously identified an Alu-derived Intronic Splicing enhancer (ISE) in the Ataxia Teleangectasia Mutated gene (ATM) that facilitates intron pre-mRNA processing and leads to the inclusion of a cryptic exon in the final mRNA transcript.
Tibor Pastor, Franco Pagani
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Targeting a Pre-mRNA Structure with Bipartite Antisense Molecules Modulates Tau Alternative Splicing [PDF]
, 2012 Approximately 15% of human genetic diseases are estimated to involve dysregulation of alternative pre-mRNA splicing. Antisense molecules designed to alter these and other splicing events typically target continuous linear sequences of the message.
Liu, Yang +3 more
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Pre‐mRNA splicing modulations in senescence [PDF]
Aging Cell, 2002 SummaryAging and associated diseases involve multilevel changes in the complex phenomenon of alternative splicing. Here, we review the potential genomic and environmental origins of such changes and discuss the research implications of these findings.
Eran Meshorer, Hermona Soreq
openaire +3 more sources
PLoS ONE, 2020 Pre-mRNA splicing is critical for achieving required amounts of a transcript at a given time and for regulating production of encoded protein. A given pre-mRNA may be spliced in many ways, or not at all, giving rise to multiple gene products.
Hsin-Hao T Hsiao +5 more
doaj +1 more source
Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells [PDF]
, 1990 SF2, an activity necessary for 5' splice site cleavage and lariat formation during pre-mRNA splicing in vitro, has been purified to near homogeneity from HeLa cells. The purest fraction contains only two related polypeptides of 33 kD.
Conway, G. C. +2 more
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Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast.
Cold Spring Harbor Perspectives in Biology, 2019 SUMMARYNoncoding introns are removed from nuclear precursor messenger RNA (pre-mRNA) in a two-step phosphoryl transfer reaction by the spliceosome, a dynamic multimegadalton enzyme.
C. Plaschka, A. Newman, K. Nagai
semanticscholar +1 more source
Journal of Pharmacological Sciences, 2016 The two-pore domain K+ channel K2P5.1 has been implicated in the pathogenesis of autoimmune diseases. We investigated the changes in K2P5.1 activity caused by a defect in normal pre-mRNA splicing in concanavalin A-activated mouse splenic CD4+ T cells ...
Kazutaka Tagishi +6 more
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Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m7 guanosine cap [PDF]
, 2001 In eukaryotes the majority of mRNAs have an m7G cap that is added cotranscriptionally and that plays an important role in many aspects of mRNA metabolism.
Afonina E. +50 more
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A View of Pre-mRNA Splicing from RNase R Resistant RNAs
International Journal of Molecular Sciences, 2014 During pre-mRNA splicing, exons in the primary transcript are precisely connected to generate an mRNA. Intron lariat RNAs are formed as by-products of this process.
H. Suzuki, T. Tsukahara
semanticscholar +1 more source