Results 61 to 70 of about 203,728 (290)
CDK11 Complexes Promote Pre-mRNA Splicing [PDF]
Journal of Biological Chemistry, 2003 The PITSLRE protein kinases, hereafter referred to as CDK11 because of their association with the cyclin L regulatory partner, belong to large molecular weight protein complexes that contain RNA polymerase II. These CDK11(p110) complexes have been reported to influence transcription as well as interact with the general pre-mRNA-splicing factor RNPS1 ...
Dongli, Hu +4 more
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Splicing-coupled 3' end formation requires a terminal splice acceptor site, but not intron excision [PDF]
, 2012 Splicing of human pre-mRNA is reciprocally coupled to 3′ end formation by terminal exon definition, which occurs co-transcriptionally. It is required for the final maturation of most human pre-mRNAs and is therefore important to understand.
Davidson, L., West, S.
core +3 more sources
Molecular Oncology, EarlyView.The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués +3 more
wiley +1 more source
Development of an in vitro pre-mRNA splicing assay using plant nuclear extract
Plant Methods, 2018 Background Pre-mRNA splicing is an essential post-transcriptional process in all eukaryotes. In vitro splicing systems using nuclear or cytoplasmic extracts from mammalian cells, yeast, and Drosophila have provided a wealth of mechanistic insights into ...
Mohammed Albaqami, Anireddy S. N. Reddy
doaj +1 more source
Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing. [PDF]
PLoS Genetics, 2014 The steroid hormone ecdysone coordinates insect growth and development, directing the major postembryonic transition of forms, metamorphosis. The steroid-deficient ecdysoneless1 (ecd1) strain of Drosophila melanogaster has long served to assess the ...
Ann-Katrin Claudius +4 more
doaj +1 more source
Multiple splicing factors are released from endogenous complexes during in vitro pre-mRNA splicing [PDF]
, 1989 Pre-mRNA splicing occurs in a macromolecular complex called the spliceosome. Efforts to isolate spliceosomes from in vitro splicing reactions have been hampered by the presence of endogenous complexes that copurify with de novo spliceosomes formed on ...
Conway, G. C. +3 more
core
Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells [PDF]
, 1990 SF2, an activity necessary for 5' splice site cleavage and lariat formation during pre-mRNA splicing in vitro, has been purified to near homogeneity from HeLa cells. The purest fraction contains only two related polypeptides of 33 kD.
Conway, G. C. +2 more
core +1 more source
Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer
Molecular Oncology, EarlyView.PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta +10 more
wiley +1 more source
Molecular Genetics & Genomic Medicine, 2023 Background Gitelman syndrome (GS) is a type of salt‐losing tubular disease, most of which is caused by SLC12A3 gene variants, and missense variants account for the majority.
Xiaomeng Shi +10 more
doaj +1 more source
α-Globin pre-mRNA splicing, revisited [PDF]
Blood, 2019 In this issue of Blood, Ji et al have identified a novel regulatory mechanism that ensures proper splicing of human α-globin pre-messenger RNA (pre-mRNA).
openaire +2 more sources