The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2. [PDF]
PLoS Pathogens, 2016 The origin recognition complex (ORC) coordinates a series of events that lead to initiation of DNA strand duplication. As a nuclear double stranded DNA plasmid, the papillomavirus (PV) genome resembles a mini-chromosome in infected cells. To initiate its
Marsha DeSmet +5 more
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The role of DDK and Treslin–MTBP in coordinating replication licensing and pre-initiation complex formation [PDF]
Open Biology, 2021 Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein.
Ilaria Volpi +3 more
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A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis [PDF]
Cell Reports, 2018 Summary: DNA replication in the embryo of Xenopus laevis changes dramatically at the mid-blastula transition (MBT), with Y RNA-independent random initiation switching to Y RNA-dependent initiation at specific origins.
Christo P. Christov +6 more
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Comparative analysis of pre‐replication complex proteins in transformed and normal cells [PDF]
Journal of Cellular Biochemistry, 2011 AbstractThis study examines the abundance of the major protein constituents of the pre‐replication complex (pre‐RC), both genome‐wide and in association with specific replication origins, namely the lamin B2, c‐myc, 20mer1, and 20mer2 origins. Several pre‐RC protein components, namely ORC1‐6, Cdc6, Cdt1, MCM4, MCM7, as well as additional replication ...
Domenic Di Paola +1 more
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Mcm3 Is Polyubiquitinated during Mitosis before Establishment of the Pre-replication Complex [PDF]
Journal of Biological Chemistry, 2002 To ensure fidelity in genome duplication, eukaryotes restrict DNA synthesis to once every cell division by a cascade of regulated steps. Central to this cascade is the periodic assembly of the hexameric MCM2-7 complex at replication origins. However, in Saccharomyces cerevisiae, only a fraction of each MCM protein is able to assemble into hexamers and ...
Irene H. Cheng +2 more
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Emerging Roles for Ciz1 in Cell Cycle Regulation and as a Driver of Tumorigenesis [PDF]
Biomolecules, 2016 Precise duplication of the genome is a prerequisite for the health and longevity of multicellular organisms. The temporal regulation of origin specification, replication licensing, and firing at replication origins is mediated by the cyclin-dependent ...
Tekle Pauzaite +3 more
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Electron Microscopic Studies of the Eukaryotic Pre-Replicative Mcm2-7 Complex [PDF]
Microscopy and Microanalysis, 2013 Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.
Fabienne Beuron +4 more
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Selective interactions at pre-replication complexes categorize baseline and dormant origins [PDF]
Nature CommunicationsDNA synthesis in metazoans initiates within a select group of replication origins (baseline origins), whereas other (dormant) origins do not initiate replication despite recruiting apparently indistinguishable pre-replication complexes.
Bhushan L. Thakur +8 more
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Chromatin-dependent pre-replication complex positioning and activation in mammals
, 2017 Chaque division cellulaire requiert une duplication précise du génome. Des dizaines de milliers de sites d’initiation de la réplication d’ADN (origines de réplication) sont impliqués dans la réplication complète du génome humain. L’activation des origines de réplication est régulée précisément et des études génomiques extensives ont démontré la ...
Nina Kirstein
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An essential role for Orc6 in DNA replication through maintenance of pre‐replicative complexes [PDF]
The EMBO Journal, 2006 The heterohexameric origin recognition complex (ORC) acts as a scaffold for the G(1) phase assembly of pre-replicative complexes (pre-RC). Only the Orc1-5 subunits appear to be required for origin binding in budding yeast, yet Orc6 is an essential protein for cell proliferation.
Jeffrey W Semple +8 more
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