The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology [PDF]
, 2015 The interleukin enhancer binding factors ILF2 (NF45) and ILF3 (NF90/NF110) have been implicated in various cellular pathways, such as transcription, microRNA (miRNA) processing, DNA repair, and translation, in mammalian cells.
Badertscher, Lukas +5 more
core +1 more source
Biomolecules, 2018 In eukaryotes, 18S, 5.8S, and 28S rRNAs are transcribed as precursor molecules that undergo extensive modification and nucleolytic processing to form the mature rRNA species. Central in the process are the small nucleolar RNAs (snoRNAs).
Kasper L. Andersen, Henrik Nielsen
doaj +1 more source
Quantitative Detection of Syntrophic Fatty Acid-degrading Bacterial Communities in Methanogenic Environments [PDF]
, 2015 In methanogenic habitats, volatile fatty acids (VFA), such as propionate and butyrate, are major intermediates in organic matter degradation. VFA are further metabolized to H2, acetate and CO2 by syntrophic fatty acid-degrading bacteria (SFAB) in ...
Maki, James +2 more
core +2 more sources
A snoRNA modulates mRNA 3' end processing and regulates the expression of a subset of mRNAs. [PDF]
, 2017 mRNA 3' end processing is an essential step in gene expression. It is well established that canonical eukaryotic pre-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-acting proteins. However, it is unknown
Ding, Junjun +14 more
core +1 more source
Memorias do Instituto Oswaldo Cruz, 2012 The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family.
Mario Gustavo Mayer +3 more
doaj +1 more source
H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation
Nature CommunicationsRibosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 ...
Paulina Fischer +8 more
doaj +1 more source
Nuclear Surveillance and Degradation of Hypomodified Initiator tRNA\u3csup\u3eMet\u3c/sup\u3e in \u3cem\u3eS. cerevisiae\u3c/em\u3e [PDF]
, 2004 The tRNA m1A58 methyltransferase is composed of two subunits encoded by the essential genes TRM6 and TRM61 (formerly GCD10 and GCD14). The trm6-504 mutation results in a defective m1A methyltransferase (Mtase) and a temperature-sensitive growth phenotype
Anderson, James T. +5 more
core +1 more source
The Microbial Ecology of Bacterial Vaginosis: A Fine Scale Resolution Metagenomic Analysis [PDF]
, 2010 The vaginal microbiota play an important protective role in maintaining the health of women. Disruption of the mutualistic relationship that exists between bacterial communities in the vagina and their hosts can lead to bacterial vaginosis (BV), a ...
Jacques Ravel, Larry J. Forney
core +2 more sources
The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool [PDF]
, 2018 Background: Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion.
de Vrieze, Jo +3 more
core +2 more sources
Protocol to analyze tRNA and rRNA processing using biotin-labeled probes
STAR ProtocolsSummary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells.
Sseu-Pei Hwang +2 more
doaj +1 more source