Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing [PDF]
RNA Biology, 2023 The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the U3 Associated Proteins (UTPs) are a small subunit processome subcomplex that coordinate the first two ...
Mason A. McCool +8 more
doaj +2 more sources
Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processing. [PDF]
PLoS ONE, 2023 The biogenesis of ribosomes requires tightly controlled transcription and processing of pre-rRNA which comprises ribosomal RNAs forming the core of large and small ribosomal subunits.
Anastasiia Moraleva +9 more
doaj +2 more sources
Regulation of Pre-rRNA Processing in Plant: Mechanisms, Plasticity, and Developmental Implications [PDF]
PlantsRibosome biogenesis is a fundamental process underlying plant growth, development, and environmental adaptation, and processing of precursor rRNA (pre-rRNA) represents one of its most critical regulatory steps.
Nier Chen +3 more
doaj +2 more sources
Protocol to analyze tRNA and rRNA processing using biotin-labeled probes [PDF]
STAR ProtocolsSummary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells.
Sseu-Pei Hwang +2 more
doaj +2 more sources
BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing [PDF]
iScience, 2020 Summary: The transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II.
Marlene Cervantes +5 more
doaj +2 more sources
Knock-Down of a Novel snoRNA in Tetrahymena Reveals a Dual Role in 5.8S rRNA Processing and Generation of a 26S rRNA Fragment [PDF]
Biomolecules, 2018 In eukaryotes, 18S, 5.8S, and 28S rRNAs are transcribed as precursor molecules that undergo extensive modification and nucleolytic processing to form the mature rRNA species. Central in the process are the small nucleolar RNAs (snoRNAs).
Kasper L. Andersen, Henrik Nielsen
doaj +2 more sources
SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing [PDF]
Nature Communications, 2016 SIRT7 is a protein deacetylase with important roles in rRNA synthesis, ribosome biogenesis and cell proliferation. Here the authors show a role of SIRT7 in rRNA maturation via deacetylation of U3-55k, a core component of the U3 snoRNP complex.
Sifan Chen +6 more
doaj +2 more sources
PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]
, 2012 As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Ameet J. Pinto +2 more
core +24 more sources
RNA Biology, 2023 Ribosomal RNA (rRNA) processing and maturation are fundamentally important for ribosome biogenesis, but the mechanisms in archaea, the third form of life, remains largely elusive.
Lei Qi +6 more
doaj +1 more source
RNA Biology, 2022 Transcription and processing of 45S rRNAs in the nucleolus are keystones of ribosome biogenesis. While these processes are severely impacted by stress conditions in multiple species, primarily upon heat exposure, we lack information about the molecular ...
T. Darriere +11 more
doaj +1 more source