Results 1 to 10 of about 136,829 (254)

Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing [PDF]

RNA Biology, 2023
The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the U3 Associated Proteins (UTPs) are a small subunit processome subcomplex that coordinate the first two ...
Mason A. McCool, Carson J. Bryant, Hannah Huang, Lisa M. Ogawa, Katherine I. Farley-Barnes, Samuel B. Sondalle, Laura Abriola, Yulia V. Surovtseva, Susan J. Baserga   +8 more
doaj   +2 more sources

Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processing. [PDF]

PLoS ONE, 2023
The biogenesis of ribosomes requires tightly controlled transcription and processing of pre-rRNA which comprises ribosomal RNAs forming the core of large and small ribosomal subunits.
Anastasiia Moraleva, Alexander Deryabin, Maria Kordyukova, Mikhail Polzikov, Kseniya Shishova, Kira Dobrochaeva, Yury Rubtsov, Maria Rubtsova, Olga Dontsova, Olga Zatsepina   +9 more
doaj   +2 more sources

Regulation of Pre-rRNA Processing in Plant: Mechanisms, Plasticity, and Developmental Implications [PDF]

Plants
Ribosome biogenesis is a fundamental process underlying plant growth, development, and environmental adaptation, and processing of precursor rRNA (pre-rRNA) represents one of its most critical regulatory steps.
Nier Chen, Shiyi Huang, Beixin Mo, Wei Xiong   +3 more
doaj   +2 more sources

Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing. [PDF]

RNA, 2022
In eukaryotes, the nucleolus is the site of ribosome biosynthesis, an essential process in all cells. While human ribosome assembly is largely evolutionarily conserved, many of the regulatory details underlying its control and function have not yet been well-defined.
McCool MA, Buhagiar AF, Bryant CJ, Ogawa LM, Abriola L, Surovtseva YV, Baserga SJ.   +6 more
europepmc   +3 more sources

RECQ5 mediates pre-rRNA processing in nucleolus. [PDF]

Nucleic Acids Res
Abstract RECQ5 is a member of the RECQ helicase family that maintains genomic stability. However, the molecular mechanism of RECQ5 in this biological process remains elusive. Here, we show that RECQ5 localizes in the dense fibrillar component of nucleolus and associates with several pre-rRNA processing factors. It recognizes pre-rRNA and
Ma Y, Yu X.
europepmc   +3 more sources

Protocol to analyze tRNA and rRNA processing using biotin-labeled probes [PDF]

STAR Protocols
Summary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells.
Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt   +2 more
doaj   +2 more sources

The nucleoid as a site of rRNA processing and ribosome assembly [PDF]

Frontiers in Plant Science, 2014
Protein biosynthesis is one of the key elements of gene expression in living cells. All proteins are synthesized on ribonucleoprotein complexes, the ribosomes. In bacteria and their derivatives—eukaryotic mitochondria and plastids—ribosomes consist of a small and a large subunit which together comprise more than 50 ribosomal proteins and usually two to
Alexandra-Viola eBohne
doaj   +3 more sources

Nop53p interacts with 5.8S rRNA co‐transcriptionally, and regulates processing of pre‐rRNA by the exosome [PDF]

FEBS Journal, 2008
In eukaryotes, pre‐rRNA processing depends on a large number of nonribosomal trans‐acting factors that form intriguingly organized complexes. One of the early stages of pre‐rRNA processing includes formation of the two intermediate complexes pre‐40S and pre‐60S, which then form the mature ribosome subunits. Each of these complexes contains specific pre‐
Glaucia Maria Machado-Santelli, Carla C Oliveira   +1 more
exaly   +3 more sources

Duration of the first steps of the human rRNA processing [PDF]

Nucleus, 2013
Processing of rRNA in mammalian cells includes a series of cleavages of the primary 47S transcript and results in producing three rRNAs: 18S, 28S and 5.8S. The sequence of the main processing events in human cells has been established, but little is yet known about the dynamics of this process, especially the dynamics of its early stages.
Evgeny Smirnov, Lubomír Kováčik, Otakar Raska   +2 more
exaly   +3 more sources

Coordinated Control of rRNA Processing by RNA Polymerase I. [PDF]

Trends Genet, 2019
Ribosomal RNA (rRNA) is co- and post-transcriptionally processed into active ribosomes. This process is dynamically regulated by direct covalent modifications of the polymerase that synthesizes the rRNA, RNA polymerase I (Pol I), and by interactions with cofactors that influence initiation, elongation, and termination activities of Pol I.
Scull CE, Schneider DA.
europepmc   +4 more sources