Structural transitions during large ribosomal subunit maturation analyzed by tethered nuclease structure probing in S. cerevisiae. [PDF]
PLoS ONE, 2017 Yeast large ribosomal subunit (LSU) precursors are subject to substantial changes in protein composition during their maturation due to coordinated transient interactions with a large number of ribosome biogenesis factors and due to the assembly of ...
Gisela Pöll +8 more
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SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs [PDF]
, 1990 SSB-1, the yeast single-strand RNA-binding protein, is demonstrated to be a yeast nucleolar-specific, silver-binding protein. In double-label immunofluorescence microscopy experiments antibodies to two other nucleolar proteins, RNA Pol I 190-kD and ...
Abelson, John +3 more
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Regulation of rRNA Processing: A Role for a Unique GTPase [PDF]
Molecular Cell, 2005 Recent findings by Karbstein et al. (2005 [this issue of Molecular Cell]) reveal that association of the preribosomal biosynthesis factors U3 snoRNA and Rcl1p is controlled by the GTPase Bms1p, suggesting that regulatory events are involved in the formation of ribosome biogenesis complexes.
Dutca, Laura M., Culver, Gloria M.
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Cell Reports, 2015 The 5S ribonucleoprotein particle (RNP) complex, consisting of RPL11, RPL5, and 5S rRNA, is implicated in p53 regulation under ribotoxic stress. Here, we show that the 5S RNP contributes to p53 activation and promotes cellular senescence in response to ...
Kazuho Nishimura +9 more
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A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast. [PDF]
PLoS ONE, 2011 Ribogenesis is a multistep error-prone process that is actively monitored by quality control mechanisms. How ribosomal RNA synthesis, pre-rRNA processing and nucleolar surveillance are integrated is unclear.
Nathalie Leporé, Denis L J Lafontaine
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Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA [PDF]
, 2012 Ribosomal RNA undergoes various modifications to optimize ribosomal structure and expand the topological potential of RNA. The most common nucleotide modifications in ribosomal RNA (rRNA) are pseudouridylations and 2'-O methylations (Nm), performed by H ...
Entian, Karl-Dieter +5 more
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Rok1p Is a Putative RNA Helicase Required for rRNA Processing [PDF]
Molecular and Cellular Biology, 1997 The synthesis of ribosomes involves many small nucleolar ribonucleoprotein particles (snoRNPs) as transacting factors. Yeast strains lacking the snoRNA, snR10, are viable but are impaired in growth and delayed in the early pre-rRNA cleavages at sites A0, A1, and A2, which lead to the synthesis of 18S rRNA.
Venema, J +4 more
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Processing human urine and ureteral stents for 16S rRNA amplicon sequencing
STAR Protocols, 2021 Summary: Ureteral stents are commonly used medical devices that harbor a unique and patient-specific microbial community. This protocol describes an optimized procedure for high-quality DNA extraction from both urine and ureteral stent samples for the ...
Kait F. Al, Jeremy P. Burton
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Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2016 Pan Zhu, Yuqiu Wang, Nanxun Qin
exaly +2 more sources
A Quantitative Sequencing Framework for Absolute Abundance Measurements of Mucosal and Lumenal Microbial Communities [PDF]
, 2020 A fundamental goal in microbiome studies is determining which microbes affect host physiology. Standard methods for determining changes in microbial taxa measure relative, rather than absolute abundances.
Barlow, Jacob T. +2 more
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