Results 51 to 60 of about 158,711 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Pathogenic and Apathogenic Strains of Lymphocytic Choriomeningitis Virus Have Distinct Entry and Innate Immune Activation Pathways

Viruses
Lymphocytic choriomeningitis virus (LCMV) and Lassa virus (LASV) share many genetic and biological features including subtle differences between pathogenic and apathogenic strains.
Dylan M. Johnson, Nittaya Khakhum, Min Wang, Nikole L. Warner, Jenny D. Jokinen, Jason E. Comer, Igor S. Lukashevich   +6 more
doaj   +1 more source

Modeling the Combined Effect of pH, Protein Content, and Mexican Oregano Essential Oil Against Food Spoilage Molds

Frontiers in Sustainable Food Systems, 2020
Hurdle technologies are among the novel food processing techniques for food preservation, where there is a combination of mild antimicrobial factors, which can interact on the inhibition of microbial spoilage.
Anahí Levario-Gómez, Raúl Ávila-Sosa, Néstor Gutiérrez-Méndez, Aurelio López-Malo, Guadalupe Virginia Nevárez-Moorillón   +4 more
doaj   +1 more source

Cardinal Parameters Model in Food Microbiological Monitoring: A Review

Shipin gongye ke-ji, 2022
Outbreaks of foodborne diseases prompted researchers to look for faster and more effective ways to monitor microorganisms in food. Predictive microbial models quantify the impact of intrinsic and extrinsic factors on microbial behavior in food for food ...
Yuting SHEN, Shaowen LI, Xianrong MENG
doaj   +1 more source

Identifying which septic patients have increased mortality risk using severity scores:a cohort study [PDF]

, 2014
Background: Early aggressive therapy can reduce the mortality associated with severe sepsis but this relies on prompt recognition, which is hindered by variation among published severity criteria. Our aim was to test the performance of different severity
Davey, Peter G, Evans, Josie Mm, Guthrie, Bruce, Marwick, Charis A, McLeod, Shaun R, Pringle, Jan Ec   +5 more
core   +4 more sources

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study. [PDF]

, 2019
Antimicrobial resistance (AMR) poses a threat to public health. Clinical microbiology laboratories typically rely on culturing bacteria for antimicrobial-susceptibility testing (AST).
Aller, SD, Bruchmann, S, Clark, T, Coello Pelegrin, A, Cormican, M, Diez Benavente, E, Doyle, RM, Ellington, MJ, Harris, KA, Huggett, JF, McGrath, E, Moran-Gilad, J, Motro, Y, O'Sullivan, DM, Phelan, J, Phuong Thuy Nguyen, T, Shaw, LP, Stabler, RA, van Belkum, A, van Dorp, L, Woodford, N   +20 more
core   +4 more sources

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Quality of Tenebrio molitor Powders: Effects of Four Processes on Microbiological Quality and Physicochemical Factors

Foods, 2023
Tenebrio molitor, the first edible insect approved as a novel food in the EU, is a promising candidate for alternative protein sources, implementing circular and sustainable production systems. This study aims to determine the microbiological quality and
Xin Yan, Sophie Laurent, Isabelle Hue, Sylvie Cabon, Joelle Grua-Priol, Vanessa Jury, Michel Federighi, Geraldine Boué   +7 more
doaj   +1 more source

In Situ 3D Bioprinting: Impact of Cross‐Linking on the Adhesive Properties of Hydrogels

Advanced Functional Materials, EarlyView.
In situ 3D bioprinting enables the direct deposition of cell‐laden, adhesive biomaterials for on‐site tissue regeneration. This review provides a comprehensive overview of how cross‐linking influences the bioadhesive properties of hydrogels used in 3D bioprinting, highlighting cross‐linking triggers, bioadhesion mechanisms, polymer interpenetration ...
Odile Romero Fernandez, Mulazim Hussain Asim, Shumaila Arshad, Gergely Kali, Andreas Bernkop‐Schnürch   +4 more
wiley   +1 more source

Omnibus Modeling of Listeria monocytogenes Growth Rates at Low Temperatures

Foods, 2021
Listeria monocytogenes is a pathogen of considerable public health importance with a high case fatality. L. monocytogenes can grow at refrigeration temperatures and is of particular concern for ready-to-eat foods that require refrigeration.
Vincenzo Pennone, Ursula Gonzales-Barron, Kevin Hunt, Vasco Cadavez, Olivia McAuliffe, Francis Butler   +5 more
doaj   +1 more source