Results 21 to 30 of about 18,030 (201)

How to make a primordial germ cell [PDF]

Development, 2014
Primordial germ cells (PGCs) are the precursors of sperm and eggs, which generate a new organism that is capable of creating endless new generations through germ cells. PGCs are specified during early mammalian postimplantation development, and are uniquely programmed for transmission of genetic and epigenetic information to subsequent generations.
Erna, Magnúsdóttir, M Azim, Surani
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Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors

Cell Reports, 2019
Summary: In vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation of this model is the quality of the first progenitors called primordial germ cells (PGCs), which in vivo are specified during the ...
Di Chen, Na Sun, Lei Hou, Rachel Kim, Jared Faith, Marianna Aslanyan, Yu Tao, Yi Zheng, Jianping Fu, Wanlu Liu, Manolis Kellis, Amander Clark   +11 more
doaj   +1 more source

The Neonatal and Adult Human Testis Defined at the Single-Cell Level

Cell Reports, 2019
Summary: Spermatogenesis has been intensely studied in rodents but remains poorly understood in humans. Here, we used single-cell RNA sequencing to analyze human testes.
Abhishek Sohni, Kun Tan, Hye-Won Song, Dana Burow, Dirk G. de Rooij, Louise Laurent, Tung-Chin Hsieh, Raja Rabah, Saher Sue Hammoud, Elena Vicini, Miles F. Wilkinson   +10 more
doaj   +1 more source

Tracing the transitions from pluripotency to germ cell fate with CRISPR screening

Nature Communications, 2018
Primordial Germ Cell-Like Cells (PGCLCs) are an in vitro model for primordial germ cell development. Here, the authors couple a novel compound reporter with CRISPR screening to identify key genes for exit from pluripotency and acquisition of PGCLC fate ...
Jamie A. Hackett, Yun Huang, Ufuk Günesdogan, Kristjan A. Gretarsson, Toshihiro Kobayashi, M. Azim Surani   +5 more
doaj   +1 more source

Identification of Primordial Germ Cells: Cytological, Histological and Immunohistochemical Aspects

Brazilian Archives of Biology and Technology, 2015
Primordial germ cells (PGCs) constitute an embryonic cell type that migrate to gonadal precursors and form the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis.
Nazan Deniz Yön, Cansu Akbulut
doaj   +1 more source

Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus

Animals, 2021
It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population.
Dragos Scarlet, Stephan Handschuh, Ursula Reichart, Giorgia Podico, Robyn E. Ellerbrock, Sebastián Demyda-Peyrás, Igor F. Canisso, Ingrid Walter, Christine Aurich   +8 more
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Induction of pluripotency in primordial germ cells.

Histology and histopathology, 2011
Primordial germ cells (PGCs) are the founder cells of all gametes. PGCs differentiate from pluripotent epiblasts cells by mesodermal induction signals during gastrulation. Although PGCs are unipotent cells that eventually differentiate into only sperm or oocytes, they dedifferentitate to pluripotent stem cells known as embryonic germ cells (EGCs) in ...
Kimura, Tohru, Nakano, Toru
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A role for Lin28 in primordial germ-cell development and germ-cell malignancy [PDF]

Nature, 2009
The rarity and inaccessibility of the earliest primordial germ cells (PGCs) in the mouse embryo thwart efforts to investigate molecular mechanisms of germ-cell specification. stella (also called Dppa3) marks the rare founder population of the germ lineage.
West, Jason A., Viswanathan, Srinivas R., Yabuuchi, Akiko, Cunniff, Kerianne, Takeuchi, Ayumu, Park, In-Hyun, Sero, Julia E., Zhu, Hao, Perez-Atayde, Antonio, Frazier, A. Lindsay, Surani, M. Azim, Daley, George Q.   +11 more
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Heterogeneity of primordial germ cells

, 2019
Primordial germ cells (PGCs) must complete a complex and dynamic developmental program during embryogenesis to establish the germline. This process is highly conserved and involves a diverse array of tasks required of PGCs, including migration, survival, sex differentiation, and extensive epigenetic reprogramming.
Nguyen, Daniel H, Jaszczak, Rebecca G, Laird, Diana J   +2 more
openaire   +5 more sources

Transcriptional quiescence in primordial germ cells

Critical Reviews in Biochemistry and Molecular Biology, 2018
In most animal species, newly formed primordial germ cells (PGCs) acquire the special characteristics that distinguish them from the surrounding somatic cells. Proper fate specification of the PGCs is coupled with transcriptional quiescence, whether they are segregated by determinative or inductive mechanisms. Inappropriate differentiation of PGCs into
Lyubov A. Lebedeva, Konstantin V. Yakovlev, Eugene N. Kozlov, Paul Schedl, Girish Deshpande, Yulii V. Shidlovskii   +5 more
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