Results 21 to 30 of about 5,813 (150)

DIAGNOSIS OF DENGUE VIRUS INFECTIONS IN ITALY FROM NOVEMBER 2015 TO NOVEMBER 2021: A NATIONAL REFERENCE LABORATORY SURVEILLANCE REPORT

International Journal of Infectious Diseases, 2023
Intro: Dengue virus (DENV) is a mosquito-borne human pathogen endemic in 129 countries. In Italy, presence of the competent vector increases the risk of autochthonous transmission from imported cases, and a surveillance national plan for Arboviruses (PNA
C. Merakou, C. Fortuna, A. Amendola, G. Marsili, C. Argentini, C. Fiorentini, E. Benedetti, F. Riccardo, M. Del Manso, A. Bella, M.G. Caporali, P. Pezzotti, G. Venturi   +12 more
doaj   +1 more source

Evaluation of a commercial ELISA as alternative to plaque reduction neutralization test to detect neutralizing antibodies against SARS-CoV-2

Scientific Reports, 2022
High-throughput detection of neutralizing antibodies against SARS-CoV-2 presents a valuable tool for vaccine trials or investigations of population immunity.
Natalie Hofmann, Marica Grossegesse, Markus Neumann, Lars Schaade, Andreas Nitsche   +4 more
doaj   +1 more source

Triple Burden in the Democratic Republic of Congo: Climate Change, Armed Conflict, and the Silent Spread of Arboviruses. A Narrative Review. [PDF]

Health Sci Rep
ABSTRACT Background The Democratic Republic of Congo (DRC) faces a “triple burden” of public health threats which includes climate change, protracted armed conflict, and the under‐recognized spread of arboviral diseases. Arboviruses, such as dengue, chikungunya, Zika, and yellow fever, are transmitted by Aedes aegypti and Aedes albopictus mosquitoes ...
Donnang S, Yokolo H, Makeda D, Ekouo J, Shams Z, Kaptso JAD, Kamgang JS, Waffo YV, Tague C.   +8 more
europepmc   +2 more sources

Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays

Microbiology Spectrum, 2021
The evaluation of humoral protective immunity against SARS-CoV-2 remains crucial in understanding both natural immunity and protective immunity conferred by the several vaccines implemented in the fight against COVID-19.
Emelissa J. Valcourt, Kathy Manguiat, Alyssia Robinson, Yi-Chan Lin, Kento T. Abe, Samira Mubareka, Altynay Shigayeva, Zoë Zhong, Roxie C. Girardin, Alan DuPuis, Anne Payne, Kathleen McDonough, Zhen Wang, Romain Gasser, Annemarie Laumaea, Mehdi Benlarbi, Jonathan Richard, Jérémie Prévost, Sai Priya Anand, Kristina Dimitrova, Clark Phillipson, David H. Evans, Allison McGeer, Anne-Claude Gingras, Chen Liang, Martin Petric, Inna Sekirov, Muhammad Morshed, Andrés Finzi, Michael Drebot, Heidi Wood   +30 more
doaj   +1 more source

Booster mRNA Vaccination Prevents Breakthrough Severe COVID-19 Infections. [PDF]

J Med Virol
ABSTRACT This study aimed to assess the humoral immune response following administration of a BNT162b2 mRNA COVID‐19 booster dose and to evaluate its association with the development of SARS‐CoV‐2 infection after vaccination. Between July 2021 and February 2022, serum samples were collected from 313 individuals 28 days after receiving a COVID‐19 ...
Dinç HÖ, Uysal HK, Can G, Budak B, Daşdemir FO, Keskin E, Aydoğan O, Balkan İİ, Karaali R, Saribaş S, Ergin S, Saltoğlu N, Kocazeybek B.   +12 more
europepmc   +2 more sources

Validation and Establishment of the SARS-CoV-2 Lentivirus Surrogate Neutralization Assay as a Prescreening Tool for the Plaque Reduction Neutralization Test

Microbiology Spectrum, 2023
Neutralization assays are important for understanding and quantifying neutralizing antibody responses toward severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
John Merluza, Johnny Ung, Kai Makowski, Alyssia Robinson, Kathy Manguiat, Nicole Mueller, Jonathan Audet, Julie Chih-Yu Chen, James E. Strong, Heidi Wood, Alexander Bello   +10 more
doaj   +1 more source

Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays. [PDF]

PLoS ONE, 2015
Currently, a number of assays measure Orthopoxvirus neutralization with serum from individuals, vaccinated against smallpox. In addition to the traditional plaque reduction neutralization test (PRNT), newer higher throughput assays are based on ...
Irina Gates, Victoria Olson, Scott Smith, Nishi Patel, Inger Damon, Kevin Karem   +5 more
doaj   +1 more source

Agreement between ELISA and plaque reduction neutralisation assay in Detection of respiratory syncytial virus specific antibodies in a birth Cohort from Kilifi, coastal Kenya. [version 1; peer review: 2 approved, 2 approved with reservations]

Wellcome Open Research, 2019
Background: Severe disease associated with respiratory syncytial virus (RSV) infection occurs predominantly among infants under 6 months of age. Vaccines for prevention are in clinical development.
Joyce U. Nyiro, Patience K. Kiyuka, Martin N. Mutunga, Charles J. Sande, Patrick K. Munywoki, J. Anthony G. Scott, D. James Nokes   +6 more
doaj   +1 more source

Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening

Pathogens, 2023
Flavivirus infections, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), present significant global public health challenges.
Kazumi Haga, Zhenying (Nancy) Chen, Misao Himeno, Ryuichi Majima, Meng Ling Moi   +4 more
doaj   +1 more source

Additional file 1 of Comparison of measles IgG enzyme immunoassays (EIA) versus plaque reduction neutralization test (PRNT) for measuring measles serostatus: a systematic review of head-to-head analyses of measles IgG EIA and PRNT

, 2023
Additional file 1: Supplemental Table 1. PRISMA statement for a systematic literature search checklist. Supplemental Table 2A. Studies evaluating EIA compared to PRNT. Supplemental Table 2B. Studies evaluating EIA compared to PRNT. Supplementary Table 3. Mediandiagnostic accuracy of EIA compared to PRNT by assay type and study quality.
Lutz, Chelsea S., Hasan, Alvira Z., Bolotin, Shelly, Crowcroft, Natasha S., Cutts, Felicity T., Joh, Eugene, Loisate, Stacie, Moss, William J., Osman, Selma, Hayford, Kyla   +9 more
openaire   +1 more source