Results 181 to 190 of about 11,689 (228)

A simple method for cloning leishmanial promastigotes

Zeitschrift f�r Parasitenkunde Parasitology Research, 1986
A method of cloning leishmanial promastigotes is described in which mid-exponential phase cultures are diluted to contain approximately 3 X 10(3) promastigotes per ml. Hanging-drop preparations made from 0.2-0.4 microliter volumes of the diluted culture seen to contain a single promastigote are picked-up in glass capillary tubes.
D A, Evans, V, Smith
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Toxicity of bilirubin to Leishmania tropica promastigotes

Experimental Parasitology, 1976
Abstract Treatment of Leishmania tropica promastigotes with bilirubin results in loss of viability and death of the cells. A concentration of 0.1 m M is sufficient to cause complete inhibition of growth. Bilirubin inhibits the uptake of 2-deoxy- d -glucose and l -methionine. It increases the efflux of intracellularly accumulated sugars (both free
M W, Simon, J M, Rusnak, A J, Mukkada
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Leishmania donovani: A Chemically Defined Medium Suitable for Cultivation and Cloning of Promastigotes and Transformation of Amastigotes to Promastigotes

The Journal of Protozoology, 1990
ABSTRACTA chemically defined medium using commercially available α‐MEM supplemented with HEPES, L‐glutamine, D‐glucose, folic acid, D‐biotin and adenine supports the luxuriant growth and propagation of Leishmania donovani promastigotes. A peak parasite population of about 7.0 × 107/ml at stationary phase and a population doubling time of 11.4 h for ...
K, Kar, K, Mukerji, K, Naskar, A, Bhattacharya, D K, Ghosh   +4 more
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Leishmania donovani: Hemolytic Activity of Promastigotes

Experimental Parasitology, 1994
A hemolytically active component was found to be present in Leishmania donovani promastigotes for the first time. It lysed human and rabbit erythrocytes to varying degrees. The optimal pH for the activity was found to be 5.8. The rate of hemolysis was dependent on both erythrocyte and parasite concentrations.
R, Chakravarty, M C, Sharma, A K, Gupta, N, Prakash, R, Saran   +4 more
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Characterization of sinefungin-resistantLeishmania donovani promastigotes

Parasitology Research, 1993
Promastigotes resistant to sinefungin (SF), a nucleoside antibiotic that is structurally related to S-adenosylmethionine (AdoMet), were obtained starting from two cloned strains of Leishmania donovani. The resistance was induced by increasing the drug pressure gradually until promastigotes capable of growing in the presence of concentrations 10,000 ...
M A, Phelouzat, F, Lawrence, M, Robert-Gero   +2 more
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Putrescine transport system in Leishmania infantum promastigotes

Molecular and Biochemical Parasitology, 1989
A transport system for putrescine in Leishmania infantum (= L. donovani infantum) promastigotes has been identified and characterized by measuring the uptake of radioactively labelled putrescine. Putrescine uptake was time- and temperature-dependent without any accumulation taking place at 0 degrees C. Uptake of putrescine was maximal at pH values near
R, Balaña-Fouce, D, Ordóñez, J M, Alunda   +2 more
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Neutral amino acid transport in Leishmania promastigotes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1983
Neutral amino acid transport was investigated in Leishmania promastigotes. Proline and alanine transport occur against their concentration gradient although there is a very rapid (40% at 30 min) conversion of proline to alanine. Uptake of these amino acids occurs by a sodium-independent route which is completely eliminated by addition of CCCP or KCN ...
P, Bonay, B E, Cohen
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Detection of lectin activity in Leishmania promastigotes and amastigotes

Acta Tropica, 1997
Cell lysates from 16 strains of eight Leishmania species were used to test haemagglutination activity (HA) against a variety of RBC. HA was detected using native or neuraminidase-treated rabbit RBC; it was found in promastigotes of all the Leishmania strains tested and in axenic amastigotes of L. mexicana.
Svobodová, M, Bates, P A, Volf, P
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Leishmania infantum: Infection of macrophages in vitro with promastigotes

International Journal for Parasitology, 1996
Leishmania infantum promastigotes in axenic culture exhibit limited infectivity for mouse peritoneal macrophages (M phi) in vitro using standard culture conditions (37 degrees C; 95% air/5% CO2) compared to Leishmania donovani promastigotes which induce notable infections.
S, Méndez, M, Nell, J M, Alunda
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Purine metabolism in Leishmania donovani amastigotes and promastigotes

Molecular and Biochemical Parasitology, 1983
Purine metabolism in Leishmania donovani amastigotes was found to be similar to that of promastigotes with the exception of adenosine metabolism. Adenosine kinase activity in amastigotes is approximately 50-fold greater than in promastigotes. Amastigotes deaminate adenosine to inosine through adenosine deaminase, an enzyme not present in promastigotes.
D L, Looker, R L, Berens, J J, Marr
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