Results 121 to 130 of about 7,503 (174)

Lipids of Leishmania Promastigotes

The Journal of Parasitology, 1979
A chromatographic analysis of lipids of cultured promastigotes of Leishmania donovani, L. braziliensis, L. mexicana, L. tropica, L. enriettii, L. hertigi, L. adleri, and L. tarentolae showed that total lipids were 2--15% of dry wt, and neutral and polar lipids were 14--55% and 45--86% of total lipids.
D H, Beach, G G, Holz, G E, Anekwe
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Promastigote infectivity inLeishmania infantum

Parasitology Research, 1991
Different clones and subpopulations of clones of Leishmania infantum were analyzed for their infective potential in vitro. Infectivity for macrophage-like cells (P388D1) and the response to a challenge with normal human serum enabled a clear differentiation between infective and non/low-infective populations. The results were confirmed by determination
F, Grimm, R, Brun, L, Jenni
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Binding ofLeishmania promastigotes to macrophages

Zeitschrift f�r Parasitenkunde Parasitology Research, 1983
Leishmania tropica promastigotes are easily attached to and engulfed by C3H peritoneal macrophages in vitro at 37 degrees C. Different sugars at 0.3-0.5 M inhibited in vitro the attachment of L. tropica promastigotes to C3H peritoneal macrophages with lactose (Gal-beta [1 leads to 4]Glc) being the most efficient.
U, Zehavi, J, El-On, E, Pearlman, J C, Abrahams, C L, Greenblatt   +4 more
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Respiration of Leishmania mexicana amastigotes and promastigotes

Molecular and Biochemical Parasitology, 1981
Promastigotes of Leishmania mexicana mexicana recently derived from amastigotes by transformation in vitro respired at a rate (17 nmol O2/min per 10(8) parasites) 4-5 times higher than that of amastigotes, but when the difference in cell protein content between the two preparations was taken into account the rates were not significantly different (32 ...
D T Hart, K Vickerman, G H Coombs
exaly   +3 more sources

A simple method for cloning leishmanial promastigotes

Zeitschrift f�r Parasitenkunde Parasitology Research, 1986
A method of cloning leishmanial promastigotes is described in which mid-exponential phase cultures are diluted to contain approximately 3 X 10(3) promastigotes per ml. Hanging-drop preparations made from 0.2-0.4 microliter volumes of the diluted culture seen to contain a single promastigote are picked-up in glass capillary tubes.
D A, Evans, V, Smith
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Leishmania tarentolae: Proline anabolism in promastigotes

Experimental Parasitology, 1976
Abstract Using 14C-labeled substrates in conjunction with thin-layer Chromatographic radioautography, studies were done to determine the precursors for proline synthesis utilized by promastigotes of Leishmania tarentolae. The only substances in Trager's Defined Medium capable of acting as proline precursors under the conditions studied were l ...
Kenneth P. Wagner, Stuart M. Krassner
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Glucose transport in Leishmania donovani promastigotes

Molecular and Biochemical Parasitology, 1984
Mid-log phase Leishmania donovani promastigotes accumulated 2-deoxy-D-glucose (2-DOG) via a carrier mediated transport system, maintaining an apparent Km of 24.4 microM and a Vmax of 3.12 nmol mg-1 protein min-1. D-Glucose but not L-glucose competitively inhibited the 2-DOG transport with an apparent Ki of 18.7 microM.
Dan Zilberstein, Dennis M Dwyer
exaly   +3 more sources

Exploitation of the complement system by Leishmania promastigotes

Parasitology Today, 1996
Complement has long been acknowledged as an important component of host defense to extracellular pathogens. David Mosser and Andrew Brittingham here describe how Leishmania spp, intracellular pathogens of mononuclear phagocytes, exploit the opsonic and chemotactic effects of complement to initiate infection in the mammalian host.
A, Brittingham, D M, Mosser
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Heme metabolism in promastigotes of Leishmania donovani

Molecular and Cellular Biochemistry, 1997
Promastigotes of Leishmania donovani (Dd-8 strain) showed presence of important key enzymes of heme synthesizing (delta-aminolevulinic acid synthase and ferrochelatase) and degrading (heme oxygenase and biliverdin reductase) systems, classical leishmanicidal drugs viz allopurinol, amphotericin B, pentamidine and CDRI compound 93/202 inhibited the heme ...
P, Srivastava, G D, Sharma, K K, Kamboj, A K, Rastogi, V C, Pandey   +4 more
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Leishmania braziliensis: Localization of glycoproteins in promastigotes

Experimental Parasitology, 1986
Two species of glycoproteins from Leishmania braziliensis promastigotes of apparent molecular weights of 53,000 (glycoprotein 53) and 47,000 (glycoprotein 47) were localized. Four lectins with different sugar specificities bound to the blotting sheet to which the electrophoretically separated materials were transferred.
K, Nagakura, H, Tachibana, Y, Kaneda, T, Nakae   +3 more
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