Results 131 to 140 of about 7,503 (174)

Purine metabolism in Leishmania donovani amastigotes and promastigotes

Molecular and Biochemical Parasitology, 1983
Purine metabolism in Leishmania donovani amastigotes was found to be similar to that of promastigotes with the exception of adenosine metabolism. Adenosine kinase activity in amastigotes is approximately 50-fold greater than in promastigotes. Amastigotes deaminate adenosine to inosine through adenosine deaminase, an enzyme not present in promastigotes.
Randolph L Berens, J J Marr
exaly   +3 more sources

Leishmania tropica: Uptake of methionine by promastigotes

Experimental Parasitology, 1977
Abstract Promastigotes of Leishmania tropica transport methionine against a concentration gradient through a saturable system. This concentrative uptake requires metabolic energy and is sensitive to temperature and sulfhydryl reagents such as N -ethyl maleimide. Intracellular methionine exchanges with external methionine.
A J, Mukkada, M W, Simon
openaire   +2 more sources

Toxicity of bilirubin to Leishmania tropica promastigotes

Experimental Parasitology, 1976
Abstract Treatment of Leishmania tropica promastigotes with bilirubin results in loss of viability and death of the cells. A concentration of 0.1 m M is sufficient to cause complete inhibition of growth. Bilirubin inhibits the uptake of 2-deoxy- d -glucose and l -methionine. It increases the efflux of intracellularly accumulated sugars (both free
M W, Simon, J M, Rusnak, A J, Mukkada
openaire   +2 more sources

Leishmania mexicana: Energy metabolism of amastigotes and promastigotes

Experimental Parasitology, 1982
Abstract The utilisation of substrates by Leishmania mexicana amastigotes and promastigotes differed significantly. The rates of uptake and catabolism of nonesterified fatty acids were up to 10-fold higher with amastigotes. Almost all the available exogenous fatty acids were consumed during amastigote transformation and by stationary phase of ...
D T Hart, G H Coombs
exaly   +3 more sources

Ubiquinone biosynthesis in Leishmania major promastigotes

International Journal for Parasitology, 1995
Promastigotes of Leishmania major contain a ubiquinone which has a side chain made up of nine isoprene subunits (UQ9). Incorporation of radioactivity from [14C] acetate and [14C] mevalonate into ubiquinone as well as the identification of hydroxymethylglutaryl coenzyme A reductase (HMG CoA reductase), and mevalonate kinase indicate that the isoprenoid ...
G, Ranganathan, A J, Mukkada
openaire   +2 more sources

Leishmania donovani: A Chemically Defined Medium Suitable for Cultivation and Cloning of Promastigotes and Transformation of Amastigotes to Promastigotes

The Journal of Protozoology, 1990
ABSTRACTA chemically defined medium using commercially available α‐MEM supplemented with HEPES, L‐glutamine, D‐glucose, folic acid, D‐biotin and adenine supports the luxuriant growth and propagation of Leishmania donovani promastigotes. A peak parasite population of about 7.0 × 107/ml at stationary phase and a population doubling time of 11.4 h for ...
K, Kar, K, Mukerji, K, Naskar, A, Bhattacharya, D K, Ghosh   +4 more
openaire   +2 more sources

Leishmania donovani: Hemolytic Activity of Promastigotes

Experimental Parasitology, 1994
A hemolytically active component was found to be present in Leishmania donovani promastigotes for the first time. It lysed human and rabbit erythrocytes to varying degrees. The optimal pH for the activity was found to be 5.8. The rate of hemolysis was dependent on both erythrocyte and parasite concentrations.
R, Chakravarty, M C, Sharma, A K, Gupta, N, Prakash, R, Saran   +4 more
openaire   +2 more sources

Characterization of sinefungin-resistantLeishmania donovani promastigotes

Parasitology Research, 1993
Promastigotes resistant to sinefungin (SF), a nucleoside antibiotic that is structurally related to S-adenosylmethionine (AdoMet), were obtained starting from two cloned strains of Leishmania donovani. The resistance was induced by increasing the drug pressure gradually until promastigotes capable of growing in the presence of concentrations 10,000 ...
M A, Phelouzat, F, Lawrence, M, Robert-Gero   +2 more
openaire   +2 more sources

Characterization of the respiratory chain of Leishmania donovani promastigotes

Molecular and Biochemical Parasitology, 1995
Inhibition analysis of respiration of Leishmania donovani promastigotes in resting, starved and permeabilized cells in the presence of classical electron transfer complex inhibitors such as rotenone, thenoyltrifluoroacetone and antimycin demonstrated the absence of complex I component of the respiratory chain in this organism.
K R, Santhamma, A, Bhaduri
openaire   +2 more sources

Leishmania tarentolae: Streptomycin and chloramphenicol resistance of promastigotes

Experimental Parasitology, 1976
Abstract Stable mutant strains of Leishmania tarentolae promastigotes resistant to chloramphenicol (CAP) were isolated by replica-plating techniques. In addition, cell lines stress-adapted to streptomycin and to high culture temperature (33 C) were obtained.
K P, Wagner, S M, Krassner
openaire   +2 more sources