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Eosinophil chemotactic activity in Leishmania amazonensis promastigotes

Parasitology Research, 1996
Tissue eosinophilia was observed in the subcutaneous tissue of mice shortly after their inoculation not only with living but also with lysed promastigotes of Leishmania amazonensis. Intraperitoneal inoculation of lysed promastigotes from five different Leishmania species (L. donovani, L. chagasi, L. tropica, L. amazonensis, and L. braziliensis) induced
S, Saito   +5 more
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Putrescine transport system in Leishmania infantum promastigotes

Molecular and Biochemical Parasitology, 1989
A transport system for putrescine in Leishmania infantum (= L. donovani infantum) promastigotes has been identified and characterized by measuring the uptake of radioactively labelled putrescine. Putrescine uptake was time- and temperature-dependent without any accumulation taking place at 0 degrees C. Uptake of putrescine was maximal at pH values near
R, Balaña-Fouce   +2 more
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Neutral amino acid transport in Leishmania promastigotes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1983
Neutral amino acid transport was investigated in Leishmania promastigotes. Proline and alanine transport occur against their concentration gradient although there is a very rapid (40% at 30 min) conversion of proline to alanine. Uptake of these amino acids occurs by a sodium-independent route which is completely eliminated by addition of CCCP or KCN ...
P, Bonay, B E, Cohen
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Leishmania infantum: Infection of macrophages in vitro with promastigotes

International Journal for Parasitology, 1996
Leishmania infantum promastigotes in axenic culture exhibit limited infectivity for mouse peritoneal macrophages (M phi) in vitro using standard culture conditions (37 degrees C; 95% air/5% CO2) compared to Leishmania donovani promastigotes which induce notable infections.
S, Méndez, M, Nell, J M, Alunda
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Electrophoretic analysis of the polypeptides of Leishmania amastigotes and promastigotes

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1986
The polypeptides of Leishmania mexicana mexicana (M379), L. m. amazonensis (LV78), L. major (LV39) and L. d. donovani (LV39) amastigotes and cultured promastigotes have been analysed by SDS-polyacrylamide gel electrophoresis. The polypeptide banding patterns of the promastigotes of the four species were quite similar, but distinct differences were ...
M F, Pupkis, G H, Coombs
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Cross-reacting surface antigens on Leishmania promastigotes

International Journal for Parasitology, 1983
Abstract Three strains of Leishmania donovani from America, India and Kenya and a strain of Leishmania mexicana were grown in culture as promastigotes. The parasites werw surface labelled by lactoperoxide-catalysed iodination and the surface proteins analysed by polyacrylamide gel electrophoresis and autoradiography.
Ramasamy, R, Kar, SK, Jamnadas, H
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Monoclonal antibodies to Leishmania mexicana promastigote antigens II. Cellular localization of antigens in promastigotes and infected macrophages

Journal of Cell Science, 1991
ABSTRACT In the accompanying paper by IIg et al., it was shown that Leishmania mexicana promastigotes covalently modify a secreted acid phosphatase and other proteins by carbohydrate epitopes characteristic for lipophosphoglycan (LPG). In this study, the reaction of the anti-LPG monoclonal antibodies (mAbs AP3 and L7.25) and of mAb L3.13,
Stierhof, Y.   +5 more
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Arginine Catabolism by Leishmania donovani Promastigotes

The Journal of Protozoology, 1992
ABSTRACT Leishmania donovani promastigotes were grown to late log phase, washed and resuspended in iso‐osmotic buffer containing L‐arginine, and the rate of urea formation was then measured under various conditions. Addition of glucose or mannose activated urea formation, whereas 2‐deoxyglucose inhibited and 6‐deoxyglucose had no effect.
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