Results 51 to 60 of about 1,452,849 (304)

Reverse Zymmogram Analysis for the Detection of Protease Inhibitor Activity

Bio-Protocol, 2013
This protocol describes a gel-based procedure to detect protease inhibitor activity. In this method gelatin is used as a substrate for proteolysis and is copolymerized within the polyacrylamide matrix. Protein extracts are fractioned by SDS-PAGE and then
Lilia Bernal, Florencia García-Campusano, Edgar Nájera, Felipe Cruz-García   +3 more
doaj   +1 more source

Gag-Protease Sequence Evolution Following Protease Inhibitor Monotherapy Treatment Failure in HIV-1 Viruses Circulating in East Africa [PDF]

, 2015
Around 2.5 million HIV-infected individuals failing first-line therapy qualify for boosted protease inhibitor (bPI)-based second-line therapy globally. Major resistance mutations are rarely present at treatment failure in patients receiving bPI and the ...
Kapaata, Anne, Spyer, M., Gupta, RK, Pillay, D., Spyer, M, Lyagoba, F., Goodall, Ruth L, Gupta, R.K., Gilks, C.F., Pillay, Deenan, DART Virology Group, Goodall, R.L., Thiltgen, G., Sutherland, Katherine A., Lyagoba, Fred, Dunn, David, Sutherland, K.A., McCormick, A.L., Gilks, CF, Gilks, Charles F, Kaleebu, Pontiano, Gupta, Ravindra K., Kapaata, A, Lyagoba, F, Kaleebu, P., Sutherland, Katherine A, McCormick, Adele, Pillay, D, Goodall, RL, Kaleebu, P, Thiltgen, G, Goodall, Ruth L., Kityo, C., Dunn, D., Dunn, D, Sutherland, KA, Gupta, Ravindra K, Gilks, Charles F., Kityo, C, Spyer, Moira, Kapaata, A., Thiltgen, Geant, DART Trial Team, Kityo, Cissy, McCormick, A   +44 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Potent, multi-target serine protease inhibition achieved by a simplified β-sheet motif.

PLoS ONE, 2019
Engagement of an extended β-sheet is a common substrate/inhibitor interaction at the active site of serine proteases and is an important feature of Laskowski mechanism inhibitors that present a substrate-like loop to a target protease.
Xingchen Chen, Blake T Riley, Simon J de Veer, David E Hoke, Jessica Van Haeften, Darren Leahy, Joakim E Swedberg, Maria Brattsand, Perry J Hartfield, Ashley M Buckle, Jonathan M Harris   +10 more
doaj   +1 more source

Matriptase Complexes and Prostasin Complexes with HAI-1 and HAI-2 in Human Milk: Significant Proteolysis in Lactation.

PLoS ONE, 2016
Significant proteolysis may occur during milk synthesis and secretion, as evidenced by the presence of protease-protease inhibitor complex containing the activated form of the type 2 transmembrane serine protease matriptase and the transmembrane Kunitz ...
Chih-Hsin Lai, Ying-Jung J Lai, Feng-Pai Chou, Hsiang-Hua D Chang, Chun-Che Tseng, Michael D Johnson, Jehng-Kang Wang, Chen-Yong Lin   +7 more
doaj   +1 more source

A cysteine protease inhibitor GC376 displays potent antiviral activity against coxsackievirus infection

Current Research in Microbial Sciences, 2023
Infection with coxsackievirus A10 (CV-A10) can cause hand-foot-mouth disease and is also associated with severe complications, including viral pneumonia, aseptic and viral meningitis.
Yongkang Chen, Xiaohong Li, Min Wang, Yuan Li, Jun Fan, Jingjing Yan, Shuye Zhang, Lu Lu, Peng Zou   +8 more
doaj   +1 more source

Fragment Molecular Orbital Based Interaction Analyses on COVID-19 Main Protease - Inhibitor N3 Complex (PDB ID:6LU7) [PDF]

, 2020
The worldwide spread of COVID-19 (new coronavirus found in Wuhan in 2019) is an emergent issue to be tackled. In fact, a great amount of works in various fields have been made in rather short period.
Koji, Okuwaki, Yuji, Mochizuki, Ryo, Hatada, Shigenori, Tanaka, Kaori, Fukuzawa, Yuto, Komeiji, Yoshio, Okiyama   +6 more
core   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Inibidor de protease

, 2013
Os inibidores de protease evitam a maturação das proteínas virais dentro das partículas de HIVEducação Superior::Ciências da Saúde ...
Howard Hughes Medical Institute (HHMI)
core   +2 more sources

Respiratory syncytial virus (RSV) entry is inhibited by serine protease inhibitor AEBSF when present during an early stage of infection

Virology Journal, 2017
Background Host proteases have been shown to play important roles in many viral activities such as entry, uncoating, viral protein production and disease induction.
Winke Van der Gucht, Annelies Leemans, Marjorie De Schryver, Annick Heykers, Guy Caljon, Louis Maes, Paul Cos, Peter L. Delputte   +7 more
doaj   +1 more source