Results 41 to 50 of about 328,780 (311)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

A mathematical model for targeting chemicals to tissues by exploiting complex degradation

Biology Direct, 2011
Background In many biological and therapeutic contexts, it is highly desirable to target a chemical specifically to a particular tissue where it exerts its biological effect. In this paper, we present a simple, generic, mathematical model that elucidates
Grodzinsky Alan J, Pivonka Peter, Smith David W, Zhang Lihai, Gardiner Bruce S   +4 more
doaj   +1 more source

A Targeted Computational Screen of the SWEETLEAD Database Reveals FDA-Approved Compounds with Anti-Dengue Viral Activity

mBio, 2020
Affordable and effective antiviral therapies are needed worldwide, especially against agents such as dengue virus that are endemic in underserved regions.
Jasmine Moshiri, David A. Constant, Bowen Liu, Roberto Mateo, Steven Kearnes, Paul Novick, Ritika Prasad, Claude Nagamine, Vijay Pande, Karla Kirkegaard   +9 more
doaj   +1 more source

Proteases, Protease-Activated Receptors, and Atherosclerosis [PDF]

Arteriosclerosis, Thrombosis, and Vascular Biology, 2018
Coagulation activation by the TF (tissue factor) pathway plays pivotal roles in triggering platelets and precipitating acute coronary syndromes. Although dual antiplatelet therapy is effective in secondary cardiovascular prevention, combining platelet antagonism with low-dose aspirin and the oral coagulation FXa antagonist rivaroxaban has a synergistic
openaire   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Secreted retropepsin-like enzymes are essential for stress tolerance and biofilm formation in Pseudomonas aeruginosa

mBio
Proteases regulate important biological functions. Here, we present the structural and functional characterization of three previously uncharacterized aspartic proteases in Pseudomonas aeruginosa. We show that these proteases have structural hallmarks of
Justin D. Lormand, Charles H. Savelle, Jennifer K. Teschler, Eva López, Richard H. Little, Jacob G. Malone, Fitnat H. Yildiz, María J. García-García, Holger Sondermann   +8 more
doaj   +1 more source

The enzymatic activity of various organs and tissues of Far-Eastern trepang as a system of indicators of maturation and quality of products

Vestnik MGTU, 2018
The activity, substrate-inhibitory specificity, temperature and pH dependence, seasonal changes in proteolytic and nucleolytic enzyme systems of the digestive tract, muscle and reproductive trepangs tissues have been considered.
Pivnenko T. N., Pozdnyakova Yu. M. , Kovalev N. N. , Mikheev E. V. , Esipenko R. V.   +4 more
doaj   +1 more source

The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes [PDF]

, 2013
The monomer of influenza haemagglutinin is synthesized as a single polypeptide precursor that during maturation is cleaved by proteases into two active subunits. Other studies have demonstrated that the human Transmembrane Protease Serine 2 (TMPRSS2) can
Ferrara, Francesca, Böttcher-Friebertshäuser, Eva, Temperton, Nigel J., Scott, Simon D., Corti, Davide, Cattoli, Giovanni, Molesti, Eleonora, Böttcher-Frieberthäuser, Eva   +7 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

piv does not impact Pseudomonas aeruginosa virulence in Galleria mellonella

Microbiology Spectrum
Pseudomonas aeruginosa is an opportunistic human pathogen that can also infect mammals, invertebrates, and plants. Protease IV (PIV) is a secreted protease shown to be important in mammalian cornea, lung, and wound models of infection.
Rachel E. Robinson, Joshua K. Robertson, Dina A. Moustafa, Joanna B. Goldberg   +3 more
doaj   +1 more source