Results 101 to 110 of about 3,268,890 (286)

Theory and simulation of short-range models of globular protein solutions

, 2004
We report theoretical and simulation studies of phase coexistence in model globular protein solutions, based on short-range, central, pair potential representations of the interaction among macro-particles.
Allahyarov E, Barker J A, Blum L, Broide M, C Caccamo, Caccamo C, Caccamo C, Caccamo C, Caccamo C, Chandler D, Costa D, D Costa, Derjaguin B V, Eaton W A, Farnum M, G Pellicane, Giaquinta P V, Girifalco L F, Hansen J-P, Høye J S, Janin J, Kuehner D E, Lennard-Jones J E, McPherson A, Panagiotopoulos A Z, Pedersen T G, Pellicane G, Pellicane G, Piazza R, Pini D, Poon W C K, Poon W C K, Rosenfeld Y, Tessier P M, Velasco E, Verwey E J W, Waisman E, Warren P B   +37 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Interaction of divalent cations with protein PARK9 [PDF]

, 2013
Metals have been shown to play a role in the genesis and development of many neurodegenerative diseases. Park9 encoded protein can protect cells from manganese poisoning, an environmental risk factor for a Parkinson’s disease- like syndrome.
Juliano, Claudia Clelia Assunta, Medici, Serenella, Peana, Massimiliano Francesco, Remelli, Maurizio, Solinas, Costantino, Zoroddu, Maria Antonietta   +5 more
core  

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space

eLife
Fibroblast growth factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2)-dependent formation of ...
Fabio Lolicato, Julia P Steringer, Roberto Saleppico, Daniel Beyer, Jaime Fernandez-Sobaberas, Sebastian Unger, Steffen Klein, Petra Riegerová, Sabine Wegehingel, Hans-Michael Müller, Xiao J Schmitt, Shreyas Kaptan, Christian Freund, Martin Hof, Radek Šachl, Petr Chlanda, Ilpo Vattulainen, Walter Nickel   +17 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Towards the prediction of protein interaction partners using physical docking

Molecular Systems Biology, 2011
Deciphering the whole network of protein interactions for a given proteome (‘interactome’) is the goal of many experimental and computational efforts in Systems Biology.
Mark Nicholas Wass, Gloria Fuentes, Carles Pons, Florencio Pazos, Alfonso Valencia   +4 more
doaj   +1 more source

Predicting protein-protein interactions as a one-class classification problem [PDF]

, 2006
Protein-protein interactions represent a key step in understanding proteins functions. This is due to the fact that proteins usually work in context of other proteins and rarely function alone.
Alashwal, Hany, Deris, Safaai, Othman, Razib M.   +2 more
core  

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source