Results 101 to 110 of about 287,910 (304)

Protein aggregation and immunogenicity of biotherapeutics

International Journal of Pharmaceutics, 2020
Recombinant proteins are the mainstay of biopharmaceuticals. A key challenge in the manufacturing and formulation of protein biologic products is the tendency for the active pharmaceutical ingredients to aggregate, resulting in irreversible drug loss, and an increase in immunogenicity risk.
Ngoc B, Pham, Wilson S, Meng
openaire   +3 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Kinetics of Formation and Asymmetrical Distribution of Hsp104-Bound Protein Aggregates in Yeast

, 2016
Budding yeast cells have a finite replicative life span; that is, a mother cell produces only a limited number of daughter cells before it slows division and dies. Despite the gradual aging of the mother cell, all daughters are born rejuvenated and enjoy
Matifas, Audrey, Paoletti, Camille, Quintin, Sophie, Charvin, Gilles   +3 more
core   +1 more source

Exploring the interplay between zinc‐induced protein dyshomeostasis and mitochondrial dysfunction using viscosity‐sensitive sensor

Smart Molecules
Mitochondria are crucial sites for protein quality control within cells. When mitochondrial stress is triggered by protein misfolding, it can accelerate abnormal protein aggregation, potentially inducing various diseases.
Xuan He, Jiaqi Li, Wenye He, Jia Zhai, Yu Wei, Xin Zhang, Baoxing Shen, He Huang   +7 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Protein Aggregates Are Recruited to Aggresome by Histone Deacetylase 6 via Unanchored Ubiquitin C Termini

, 2012
Background: Misfolded protein aggregates are recruited to the aggresome by a protein complex consisting of histone deacetylase 6 (HDAC6). Results: The ubiquitin-binding domain (ZnF-UBP) of HDAC6 binds to ubiquitin C termini generated by ataxin-3 ...
Qiu, Wei, Sirano Dhe-Paganon, Hui Ouyang, Dong, Aiping, MacKenzie, Farrell, Ravichandran, Mani, Arrowsmith, Cheryl H, R. Grace Zhai, Ali, Yousuf O, Zhai, R. Grace, Ouyang, Hui, Mani Ravichandran, Wei Qiu, Farrell MacKenzie, Cheryl H. Arrowsmith, Aiping Dong, Yousuf O. Ali, Dhe-Paganon, Sirano   +17 more
core   +1 more source

Mesoscale fractal whey protein particles derived from microscale linear-shaped protein assemblies (Part 2): Foaming properties and heat stability

Journal of Dairy Science
: This study investigates the functionality of mesoscale whey protein particles (WPP) derived from fiber- and ribbon-shaped whey protein assemblies produced via a liquid antisolvent precipitation-based method.
Hanyu Shi, Haotian Zheng
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

A method to rapidly create protein aggregates in living cells [PDF]

, 2016
The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors ...
Mizumoto, K, Kudo, T, Wandless, TJ, Chen, L-C, Miyazaki, Y, Dey, G, Perrino, J, Meyer, T   +7 more
core  

Lumenal peroxisomal protein aggregates are removed by concerted fission and autophagy events

, 2013
We demonstrated that in the yeast Hansenula polymorpha peroxisome fission and degradation are coupled processes that are important to remove intra-organellar protein aggregates.
Manivannan, Selvambigai; id_orcid, de Boer, Rinse, Manivannan, Selvambigai, Veenhuis, Marten, van der Klei, Ida J.; id_orcid, van der Klei, Ida J.   +5 more
core   +1 more source