Results 81 to 90 of about 287,910 (304)
First Glance at Myeloid Leukaemia Factor 2 in Cardiomyocytes
Journal of Cardiovascular Development and DiseaseUnderstanding the molecular mechanisms that maintain protein homeostasis in cardiomyocytes is fundamental for the development of causal therapies for heart failure. Chaperones, the ubiquitin–proteasome system and autophagy are major regulators of cardiac
Jakob Christoph Voran +7 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Automatic segmentation and 3D feature extraction of protein aggregates in Caenorhabditis elegans
In the last years, it has become increasingly clear that neurodegenerative diseases involve protein aggregation, a process often used as disease progression readout and to develop therapeutic strategies.
Rodrigues, Nuno F. +6 more
core +1 more source
The value of fluorescence and B22 techniques as complementary approaches in protein stability analyses [PDF]
, 2011 Recombinant proteins gain more and more importance in the pharmaceutical market. One major challenge is to assure the protein integrity during manufacturing, shipping and storage.
Printz, Miriam
core
Detection and characterization of protein aggregates by fluorescence microscopy
, 2007 Aggregation may compromise the stability as well as the biological activity of protein drugs. Detection of protein aggregates is needed in the process of protein characterization and during optimization of pharmaceutical formulations.
Arvinte, Tudor +2 more
core +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Adsorption layer formation in dispersions of protein aggregates
, 2020 The review discusses recent results on the adsorption of amyloid fibrils and protein microgels at liquid/fluid interfaces. The application of the shear and dilational surface rheology, atomic force microscopy and passive particle probe tracking allowed ...
Miller, R. +6 more
core +1 more source
HspX promotes the polar localization of mycobacterial protein aggregates. [PDF]
, 2019 Misfolding of translated proteins occurs in all domains of life. In most cells, misfolded proteins coalesce in discrete aggregates at distinct cellular locations.
Meng, Xianbin +6 more
core +1 more source