Results 141 to 150 of about 287,910 (304)

Effect of protein aggregates on foaming properties of beta-lactoglobulin

, 2008
International audienceOur paper aims at determining the respective part of protein aggregates and non-aggregated proteins in the foam formation and stability of P-lactoglobulin.
Novales, Bruno, Rullier, Benedicte, Axelos M.A.V., Monique   +2 more
core   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

IMPACT OF THERMAL TREATMENT ON THE PROPERTIES OF THE PROTEIC FOAMS

Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2011
The food foams are "whipped" products that have recently experienced significant growth in the food industry. They are appreciated by consumers for their creamy texture, taste and visual aspect.
Alain Riaublanc, Catherine Loisel, Gholamreza Djelveh, Christophe Vial, Irina Nicorescu, Jack Legrand   +5 more
doaj  

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Model for Ub^ext affects on toxic protein aggregates.

, 2013
We propose that enhanced protein aggregate toxicity in Ubext-expressing cells is due to the inability of misfolded amyloidogenic proteins to be properly sequestered.
Elizabeth M. H. Tank (375559), Heather L. True (375560)   +1 more
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Metabolic phases with increasing the concentration of protein aggregates.

, 2013
a)–d) Exchange flux as a function of the protein aggregates concentration. e) and f) Relative volume fraction occupied by mitochondria e) and cytosolic enzymes f).
Alexei Vazquez (198204)
core   +1 more source

“Proteinjury”: a universal pathological mechanism mediated by cerebrospinal fluid in neurodegeneration and trauma

Frontiers in Cell and Developmental Biology
Cerebrospinal fluid (CSF) is a vital body fluid that supports the normal physiological functions of the brain and spinal cord. However, pathological conditions associated with injuries and neurodegenerative diseases lead to the accumulation of peptides ...
Vladimir F. Lazarev, Bashar A. Alhasan, Irina V. Guzhova, Boris A. Margulis   +3 more
doaj   +1 more source