Results 111 to 120 of about 583,434 (343)

Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for Composition Improvements

, 2011
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions.
I. Baianu, Valentin Prisecaru, I. C. Baianu, Valentin Prisecaru   +3 more
core   +1 more source

Lab-in-a-trench platform for real-time monitoring of cell surface protein expression [PDF]

, 2010
In this work we for the first time demonstrate real-time monitoring of the expression of membrane proteins in native, live cells, free of hydrodynamic stress at single cell resolution. This micro-optofluidic mechanism is uniquely enabled by the intricate
Dimov, Ivan Krastev, Jens Ducree, Dimov, Ivan K., Ducrée, Jens, Ivan K. Dimov, Kijanka, Gregor, Gregor Kijanka   +6 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the proteins

, 2005
The combination of complete genome sequence information with expression data enables us to characterize the relationship between a protein's evolutionary origin or functional category and its expression pattern.
Thornton, Janet M, Bhattacharyya, Sumit, Massingham, Tim, Freeman, Tom C, Freilich, Shiri, Ponsting, Hannes, Lyons, Paul A   +6 more
core   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

A bead-based western for high-throughput cellular signal transduction analyses

Nature Communications, 2016
Dissecting cellular signalling requires the analysis of large numbers of proteins. Here the authors describe DigiWest, a high-throughput protein detection method that combines the concept of western and widely-used bead array systems that allows rapid ...
Fridolin Treindl, Benjamin Ruprecht, Yvonne Beiter, Silke Schultz, Anette Döttinger, Annette Staebler, Thomas O. Joos, Simon Kling, Oliver Poetz, Tanja Fehm, Hans Neubauer, Bernhard Kuster, Markus F. Templin   +12 more
doaj   +1 more source

Fluid Biomarkers of Disease Burden and Cognitive Dysfunction in Progressive Supranuclear Palsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Identifying objective biomarkers for progressive supranuclear palsy (PSP) is crucial to improving diagnosis and establishing clinical trial and treatment endpoints. This study evaluated fluid biomarkers in PSP versus controls and their associations with regional 18F‐PI‐2620 tau‐PET, clinical, and cognitive outcomes.
Roxane Dilcher, Charles B. Malpas, Stuart J. McDonald, William T. O’Brien, Craig Despott, Kelly L. Bertram, Matthew P. Pase, Lukas Frontzkowski, Meng Law, Terence J. O’Brien, Lucy Vivash   +10 more
wiley   +1 more source

From research to clinical application multi-parameter testing: Marker panels for the early detection of complex diseases [PDF]

Journal of Medical Biochemistry, 2009
Multi Parameter Analysis can open novel diagnostic opportunities for the early diagnosis and screening of multimodal diseases like cancer. Single proteins have so far failed to describe such complex diseases.
Wild Norbert, Karl Johann, Risse Bernhard   +2 more
doaj  

Interaction modulation through arrays of clustered methyl-arginine protein modifications

Life Science Alliance, 2018
Extensively modifiable arrays of clustered arginines in a large set of human proteins function as regulatory protein interaction platforms. Quantitative immunoprecipitation assays defined two distinct cumulative binding mechanisms operating across an ...
Jonathan Woodsmith, Victoria Casado-Medrano, Nouhad Benlasfer, Rebecca L Eccles, Saskia Hutten, Christian L Heine, Verena Thormann, Claudia Abou-Ajram, Oliver Rocks, Dorothee Dormann, Ulrich Stelzl   +10 more
doaj   +1 more source

Functional and Structural Evidence of Neurofluid Circuit Aberrations in Huntington Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Disrupted neurofluid regulation may contribute to neurodegeneration in Huntington disease (HD). Because neurofluid pathways influence waste clearance, inflammation, and the distribution of central nervous system (CNS)–delivered therapeutics, understanding their dysfunction is increasingly important as targeted treatments emerge.
Kilian Hett, Abigail Dubois, Melanie Leguizamon, Alexander Song, Paula Trujillo, Colin D. McKnight, Ciaran M. Considine, Manus J. Donahue, Daniel O. Claassen   +8 more
wiley   +1 more source