Results 21 to 30 of about 5,918,106 (339)

Irreversible denaturation of maltodextrin glucosidase studied by differential scanning calorimetry, circular dichroism, and turbidity measurements. [PDF]

open access: yesPLoS ONE, 2014
Thermal denaturation of Escherichia coli maltodextrin glucosidase was studied by differential scanning calorimetry, circular dichroism (230 nm), and UV-absorption measurements (340 nm), which were respectively used to monitor heat absorption ...
Megha Goyal   +2 more
doaj   +1 more source

Highly anomalous energetics of protein cold denaturation linked to folding-unfolding kinetics. [PDF]

open access: yesPLoS ONE, 2011
Despite several careful experimental analyses, it is not yet clear whether protein cold-denaturation is just a "mirror image" of heat denaturation or whether it shows unique structural and energetic features. Here we report that, for a well-characterized
M Luisa Romero-Romero   +3 more
doaj   +1 more source

Protein Unfolding: Denaturant vs. Force [PDF]

open access: yesBiomedicines, 2021
While protein refolding has been studied for over 50 years since the pioneering work of Christian Anfinsen, there have been a limited number of studies correlating results between chemical, thermal, and mechanical unfolding. The limited knowledge of the relationship between these processes makes it challenging to compare results between studies if ...
Kelly, Colleen, Gage, Matthew J.
openaire   +3 more sources

Native protein denaturation using urea

open access: yesBioTechniques, 2017
Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar   +2 more
doaj   +1 more source

Non-Thermal Processing of a Protein Functional Beverage Using Pulsed Electric Fields: Escherichia coli Inactivation and Effect on Proteins

open access: yesBeverages, 2022
The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field
Mona Alzahrani   +2 more
doaj   +1 more source

Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]

open access: yesPLoS ONE, 2013
UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva   +10 more
doaj   +1 more source

A DSC study of zinc binding to bovine serum albumin (BSA) [PDF]

open access: yesJournal of the Serbian Chemical Society, 2007
The thermal denaturation of bovine serum albumin (BSA) is a kinetically and thermodynamically controlled process. The effects of zinc binding to bovine serum albumin (BSA), followed by differential scanning calorimetry (DSC), were investigated in this ...
SANJA OSTOJIC   +3 more
doaj   +3 more sources

POTENSI ANTIINFLAMASI JUS BUAH BELIMBING (Averrhoa carambola L.) TERHADAP DENATURASI PROTEIN IN VITRO

open access: yesBerkala Kedokteran, 2015
Starfruit (Averrhoa carambola L.) is an Asian tree that has been used in traditional medicine. Flavonoids, saponins, and tannins is thought to have antiinflammatory in inhibiting protein denaturation.
Farizka Erianti   +2 more
doaj   +1 more source

Effects of Whey Protein Denaturation and Whey Protein/Casein Ratio on the Stability and Whipping Properties of Recombined Cream [PDF]

open access: yesShipin Kexue, 2023
In this study, the effects of the denaturation degree of whey protein (WP) and whey protein/casein (WP/CN, m/m) ratio in milk powder on the emulsion stability and whipping properties of recombined cream were investigated.
LI Yue, XIE Yufei, LI Yang, LI Yan, ZHANG Liebing, YAN Jianguo
doaj   +1 more source

Effects of Wooden Breast Syndrome in Broiler Chicken on Sarcoplasmic, Myofibrillar, and Connective Tissue Proteins and Their Association with Muscle Fiber Area

open access: yesFoods, 2023
This study was conducted on chicken pectoralis major muscle with different wooden breast severity in combination with different sampling locations to investigate the effects of wooden breast syndrome on protein traits and total myofiber area, and their ...
Binbin Li   +3 more
doaj   +1 more source

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