Results 21 to 30 of about 5,918,106 (339)
Irreversible denaturation of maltodextrin glucosidase studied by differential scanning calorimetry, circular dichroism, and turbidity measurements. [PDF]
Thermal denaturation of Escherichia coli maltodextrin glucosidase was studied by differential scanning calorimetry, circular dichroism (230 nm), and UV-absorption measurements (340 nm), which were respectively used to monitor heat absorption ...
Megha Goyal +2 more
doaj +1 more source
Highly anomalous energetics of protein cold denaturation linked to folding-unfolding kinetics. [PDF]
Despite several careful experimental analyses, it is not yet clear whether protein cold-denaturation is just a "mirror image" of heat denaturation or whether it shows unique structural and energetic features. Here we report that, for a well-characterized
M Luisa Romero-Romero +3 more
doaj +1 more source
Protein Unfolding: Denaturant vs. Force [PDF]
While protein refolding has been studied for over 50 years since the pioneering work of Christian Anfinsen, there have been a limited number of studies correlating results between chemical, thermal, and mechanical unfolding. The limited knowledge of the relationship between these processes makes it challenging to compare results between studies if ...
Kelly, Colleen, Gage, Matthew J.
openaire +3 more sources
Native protein denaturation using urea
Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar +2 more
doaj +1 more source
The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field
Mona Alzahrani +2 more
doaj +1 more source
Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]
UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva +10 more
doaj +1 more source
A DSC study of zinc binding to bovine serum albumin (BSA) [PDF]
The thermal denaturation of bovine serum albumin (BSA) is a kinetically and thermodynamically controlled process. The effects of zinc binding to bovine serum albumin (BSA), followed by differential scanning calorimetry (DSC), were investigated in this ...
SANJA OSTOJIC +3 more
doaj +3 more sources
Starfruit (Averrhoa carambola L.) is an Asian tree that has been used in traditional medicine. Flavonoids, saponins, and tannins is thought to have antiinflammatory in inhibiting protein denaturation.
Farizka Erianti +2 more
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Effects of Whey Protein Denaturation and Whey Protein/Casein Ratio on the Stability and Whipping Properties of Recombined Cream [PDF]
In this study, the effects of the denaturation degree of whey protein (WP) and whey protein/casein (WP/CN, m/m) ratio in milk powder on the emulsion stability and whipping properties of recombined cream were investigated.
LI Yue, XIE Yufei, LI Yang, LI Yan, ZHANG Liebing, YAN Jianguo
doaj +1 more source
This study was conducted on chicken pectoralis major muscle with different wooden breast severity in combination with different sampling locations to investigate the effects of wooden breast syndrome on protein traits and total myofiber area, and their ...
Binbin Li +3 more
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