Results 21 to 30 of about 3,575,397 (313)
Expression of chicken lamin B2 in Escherichia coli : characterization of its structure, assembly, and molecular interactions [PDF]
, 1991 Chicken lamin B2, a nuclear member of the intermediate-type filament (IF) protein family, was expressed as a full-length protein in Escherichia coli.
Aebi, U. +11 more
core +1 more source
Structure of native glycolipoprotein filaments in honeybee royal jelly
Nature Communications, 2020 Female honeybee larvae develop into queens when they are fed exclusively with royal jelly (RJ) and major royal jelly protein 1 (MRJP1) is the main protein component of RJ. Here, the authors present the 3.5 Å cryo-EM structure of native RJ filament, where
Simone Mattei +5 more
doaj +1 more source
Studying protein-protein interactions using peptide arrays [PDF]
, 2010 Screening of arrays and libraries of compounds is well-established as a high-throughput method for detecting and analyzing interactions in both biological and chemical systems. Arrays and libraries can be composed from various types of molecules, ranging
Rito, T. +7 more
core +1 more source
Post-translational modification and regulation of human Spir protein [PDF]
, 2011 Spir proteins are the primodial members of the emerging group of actin nucleation factors, which initiate actin polymerization by binding monomeric actin to one or multiple Wiskott-Aldrich Syndrome protein (WASp) homology-2 domains.
Lakshmi, Sreeja
core +1 more source
Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase
Cell Reports, 2018 Summary: CTP synthase (CTPS) forms compartmentalized filaments in response to substrate availability and environmental nutrient status. However, the physiological role of filaments and mechanisms for filament assembly are not well understood.
Wei-Cheng Lin +22 more
doaj +1 more source
Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases
Nature Communications, 2023 Central to homologous recombination (HR) is the assembly of Rad51 recombinase on single-strand DNA (ssDNA), forming the Rad51-ssDNA filament. How the Rad51 filament is efficiently established and sustained remains partially understood. Here, we find that
Guangxue Liu +14 more
doaj +1 more source
The DinI Protein Stabilizes RecA Protein Filaments [PDF]
Journal of Biological Chemistry, 2004 When DinI is present at concentrations that are stoichiometric with those of RecA or somewhat greater, DinI has a substantial stabilizing effect on RecA filaments bound to DNA. Exchange of RecA between free and bound forms was almost entirely suppressed, and highly stable filaments were documented with several different experimental methods.
Shelley L, Lusetti +4 more
openaire +2 more sources
Heterologous protein production in filamentous fungi
Applied Microbiology and Biotechnology, 2023 Abstract Filamentous fungi are able to produce a wide range of valuable proteins and enzymes for many industrial applications. Recent advances in fungal genomics and experimental technologies are rapidly changing the approaches for the development and use of filamentous fungi as hosts for the production of both ...
Dujuan Liu +2 more
openaire +6 more sources
Interrogation of the protein-protein interactions between human BRCA2 BRC repeats and RAD51 reveals atomistic determinants of affinity [PDF]
, 2011 The breast cancer suppressor BRCA2 controls the recombinase RAD51 in the reactions that mediate homologous DNA recombination, an essential cellular process required for the error-free repair of DNA double-stranded breaks.
Rajendra, Eason +28 more
core +3 more sources
PLoS Biology, 2019 Type III protein-secretion machines are essential for the interactions of many pathogenic or symbiotic bacterial species with their respective eukaryotic hosts. The core component of these machines is the injectisome, a multiprotein complex that mediates
Emily Z Guo +8 more
doaj +1 more source