Results 31 to 40 of about 251,250 (306)

Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

Nature Communications, 2016
Mapping protein-protein interaction using crosslinking and mass spectroscopy strategies is hampered by a high rate of false-positive results. Here, the authors develop a genetically encoded photo-affinity probe for accurate identification of protein ...
Yi Yang, Haiping Song, Dan He, Shuai Zhang, Shizhong Dai, Shixian Lin, Rong Meng, Chu Wang, Peng R. Chen   +8 more
doaj   +1 more source

Comparative mapping of host–pathogen protein–protein interactions [PDF]

Current Opinion in Microbiology, 2015
Pathogens usurp a variety of host pathways via protein-protein interactions to ensure efficient pathogen replication. Despite the existence of an impressive toolkit of systematic and unbiased approaches, we still lack a comprehensive list of these PPIs and an understanding of their functional implications.
Priya S. Shah, Jason A. Wojcechowskyj, Manon Eckhardt, Nevan J. Krogan   +3 more
openalex   +5 more sources

Determining protein complex connectivity using a probabilistic deletion network derived from quantitative proteomics. [PDF]

PLoS ONE, 2009
Protein complexes are key molecular machines executing a variety of essential cellular processes. Despite the availability of genome-wide protein-protein interaction studies, determining the connectivity between proteins within a complex remains a major ...
Mihaela E Sardiu, Joshua M Gilmore, Michael J Carrozza, Bing Li, Jerry L Workman, Laurence Florens, Michael P Washburn   +6 more
doaj   +1 more source

Conservation of binding properties in protein models

Computational and Structural Biotechnology Journal, 2021
We study the models submitted to round 12 of the Critical Assessment of protein Structure Prediction (CASP) experiment to assess how well the binding properties are conserved when the X-ray structures of the target proteins are replaced by their models ...
Megan Egbert, Kathryn A. Porter, Usman Ghani, Sergei Kotelnikov, Thu Nguyen, Ryota Ashizawa, Dima Kozakov, Sandor Vajda   +7 more
doaj   +1 more source

Mapping protein–protein interactions by mass spectrometry

Mass Spectrometry Reviews
AbstractProtein–protein interactions (PPIs) are essential for numerous biological activities, including signal transduction, transcription control, and metabolism. They play a pivotal role in the organization and function of the proteome, and their perturbation is associated with various diseases, such as cancer, neurodegeneration, and infectious ...
Xiaonan Liu, Lawrence Abad, Lopamudra Chatterjee, Ileana M. Cristea, Markku Varjosalo   +4 more
openaire   +3 more sources

Cost-Effective Mapping of Genetic Interactions in Mammalian Cells

Frontiers in Genetics, 2021
Comprehensive maps of genetic interactions in mammalian cells are daunting to construct because of the large number of potential interactions, ~ 2 × 108 for protein coding genes. We previously used co-inheritance of distant genes from published radiation
Arshad H. Khan, Desmond J. Smith
doaj   +1 more source

Differential network biology

Molecular Systems Biology, 2012
Protein and genetic interaction maps can reveal the overall physical and functional landscape of a biological system. To date, these interaction maps have typically been generated under a single condition, even though biological systems undergo ...
Trey Ideker, Nevan J Krogan
doaj   +1 more source

Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers

Nature Communications, 2016
The AAA+ ATPase p97 is an essential hexameric protein with multiple protein interaction partners and cellular functions. Here, the authors use interaction mapping to examine partner proteins of this large complex, and assess the effects of these proteins
Anup Arumughan, Yvette Roske, Carolin Barth, Laura Lleras Forero, Kenny Bravo-Rodriguez, Alexandra Redel, Simona Kostova, Erik McShane, Robert Opitz, Katja Faelber, Kirstin Rau, Thorsten Mielke, Oliver Daumke, Matthias Selbach, Elsa Sanchez-Garcia, Oliver Rocks, Daniela Panáková, Udo Heinemann, Erich E. Wanker   +18 more
doaj   +1 more source

Pooled‐matrix protein interaction screens using Barcode Fusion Genetics

Molecular Systems Biology, 2016
High‐throughput binary protein interaction mapping is continuing to extend our understanding of cellular function and disease mechanisms. However, we remain one or two orders of magnitude away from a complete interaction map for humans and other major ...
Nozomu Yachie, Evangelia Petsalaki, Joseph C Mellor, Jochen Weile, Yves Jacob, Marta Verby, Sedide B Ozturk, Siyang Li, Atina G Cote, Roberto Mosca, Jennifer J Knapp, Minjeong Ko, Analyn Yu, Marinella Gebbia, Nidhi Sahni, Song Yi, Tanya Tyagi, Dayag Sheykhkarimli, Jonathan F Roth, Cassandra Wong, Louai Musa, Jamie Snider, Yi‐Chun Liu, Haiyuan Yu, Pascal Braun, Igor Stagljar, Tong Hao, Michael A Calderwood, Laurence Pelletier, Patrick Aloy, David E Hill, Marc Vidal, Frederick P Roth   +32 more
doaj   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source