Results 51 to 60 of about 292,859 (260)

Illuminating spatial and temporal organization of protein interaction networks by mass spectrometry-based proteomics

Frontiers in Genetics, 2015
Protein-protein interactions are at the core of all cellular functions and dynamic alterations in protein interactions regulate cellular signaling. In the last decade, mass spectrometry-based proteomics has delivered unprecedented insights into human ...
Jiwen eYang, Sebastian Alexander Wagner, Petra eBeli   +2 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

A Protein-Protein Interaction Map of Trypanosome ∼20S Editosomes [PDF]

Journal of Biological Chemistry, 2010
Mitochondrial mRNA editing in trypanosomatid parasites involves several multiprotein assemblies, including three very similar complexes that contain the key enzymatic editing activities and sediment at ~20S on glycerol gradients. These ~20S editosomes have a common set of 12 proteins, including enzymes for uridylyl (U) removal and addition, 2 RNA ...
Achim, Schnaufer, Meiting, Wu, Young-jun, Park, Tadashi, Nakai, Junpeng, Deng, Rose, Proff, Wim G J, Hol, Kenneth D, Stuart   +7 more
openaire   +2 more sources

Genetic interaction mapping of Aurora protein kinases in mouse oocytes

Frontiers in Cell and Developmental Biology
The Aurora Kinases (AURKs) are a family of serine-threonine protein kinases critical for cell division. Somatic cells express only AURKA and AURKB. However, mammalian germ cells and some cancer cells express all three isoforms.
Cecilia S. Blengini, Cecilia S. Blengini, Karen Schindler, Karen Schindler   +3 more
doaj   +1 more source

From nonspecific DNA-protein encounter complexes to the prediction of DNA-protein interactions. [PDF]

PLoS Computational Biology, 2009
DNA-protein interactions are involved in many essential biological activities. Because there is no simple mapping code between DNA base pairs and protein amino acids, the prediction of DNA-protein interactions is a challenging problem. Here, we present a
Mu Gao, Jeffrey Skolnick
doaj   +1 more source

Structural and Evolutionary Insights Into the Binding of Host Receptors by the Rabies Virus Glycoprotein

Frontiers in Cellular and Infection Microbiology, 2021
Rabies represents a typical model for spillover of zoonotic viral diseases among multiple hosts. Understanding the success of rabies virus (RV) in switching hosts requires the analysis of viral evolution and host interactions.
Manar E. Khalifa, Manar E. Khalifa, Leonie Unterholzner, Muhammad Munir   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Simple Ligand–Receptor Interaction Descriptor (SILIRID) for alignment-free binding site comparison

Computational and Structural Biotechnology Journal, 2014
We describe SILIRID (Simple Ligand–Receptor Interaction Descriptor), a novel fixed size descriptor characterizing protein–ligand interactions. SILIRID can be obtained from the binary interaction fingerprints (IFPs) by summing up the bits corresponding to
Vladimir Chupakhin, Gilles Marcou, Helena Gaspar, Alexandre Varnek   +3 more
doaj   +1 more source

Landscape mapping of functional proteins in insulin signal transduction and insulin resistance: a network-based protein-protein interaction analysis. [PDF]

PLoS ONE, 2011
The type 2 diabetes has increased rapidly in recent years throughout the world. The insulin signal transduction mechanism gets disrupted sometimes and it's known as insulin-resistance. It is one of the primary causes associated with type-2 diabetes.
Chiranjib Chakraborty, Sanjiban S Roy, Minna J Hsu, Govindasamy Agoramoorthy   +3 more
doaj   +1 more source