Results 61 to 70 of about 543,220 (340)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Whole proteome mapping of compound-protein interactions

Current Research in Chemical Biology, 2022
Off-target binding is one of the primary causes of toxic side effects of drugs in clinical development, resulting in failures of clinical trials. While off-target drug binding is a known phenomenon, experimental identification of the undesired protein binders can be prohibitively expensive due to the large pool of possible biological targets.
Venkat R. Chirasani, Jian Wang, Congzhou Sha, Wesley Raup-Konsavage, Kent Vrana, Nikolay V. Dokholyan   +5 more
openaire   +2 more sources

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Genetic interaction mapping of Aurora protein kinases in mouse oocytes

Frontiers in Cell and Developmental Biology
The Aurora Kinases (AURKs) are a family of serine-threonine protein kinases critical for cell division. Somatic cells express only AURKA and AURKB. However, mammalian germ cells and some cancer cells express all three isoforms.
Cecilia S. Blengini, Cecilia S. Blengini, Karen Schindler, Karen Schindler   +3 more
doaj   +1 more source

Global pairwise RNA interaction landscapes reveal core features of protein recognition

Nature Communications, 2018
RNA–protein interactions often depend on the recognition of extended RNA elements but the identification of these motifs is challenging. Here, the authors present a global integrated approach to analyze RNA–protein binding landscapes, mapping extended ...
Qin Zhou, Nikesh Kunder, José Alberto De la Paz, Alexandra E. Lasley, Vandita D. Bhat, Faruck Morcos, Zachary T. Campbell   +6 more
doaj   +1 more source

Landscape mapping of functional proteins in insulin signal transduction and insulin resistance: a network-based protein-protein interaction analysis. [PDF]

PLoS ONE, 2011
The type 2 diabetes has increased rapidly in recent years throughout the world. The insulin signal transduction mechanism gets disrupted sometimes and it's known as insulin-resistance. It is one of the primary causes associated with type-2 diabetes.
Chiranjib Chakraborty, Sanjiban S Roy, Minna J Hsu, Govindasamy Agoramoorthy   +3 more
doaj   +1 more source

Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex

iScience, 2023
Summary: Bromodomain-containing protein 8 (BRD8) is a subunit of the NuA4/TIP60-histone acetyltransferase complex. Although BRD8 has been considered to act as a co-activator of the complex, its biological role remains to be elucidated. Here, we uncovered
Kiyoshi Yamaguchi, Saya Nakagawa, Akari Saku, Yumiko Isobe, Rui Yamaguchi, Paul Sheridan, Kiyoko Takane, Tsuneo Ikenoue, Chi Zhu, Masashi Miura, Yuya Okawara, Satoru Nagatoishi, Hiroko Kozuka-Hata, Masaaki Oyama, Susumu Aikou, Yuka Ahiko, Dai Shida, Kouhei Tsumoto, Satoru Miyano, Seiya Imoto, Yoichi Furukawa   +20 more
doaj   +1 more source

Role of solvent for globular proteins in solution

, 2005
The properties of the solvent affect the behavior of the solution. We propose a model that accounts for the contribution of the solvent free energy to the free energy of globular proteins in solution.
Andrey Shiryayev, Avadh Saxena, D. S. Rhen, Daniel L. Pagan, Frank H. S., James D. Gunton, Turab Lookman   +6 more
core   +1 more source

Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches' broom disease in cacao [PDF]

, 2009
In order to increase the efficiency of cacao tree resistance to witches¿ broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes.
Carels, Nicolas, Cascardo, Julio Cezar M., Gaiotto, Fernanda Amato, Gesteira, Abelmon, Gramacho, Karina Peres, Lima, Leila S., Lopes, Uilson Vanderlei, Micheli, Fabienne, Novais, R., Pires, José Luis, Zaidan, Humberto A.   +10 more
core   +1 more source

4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase in malaria parasites and enhances atovaquone efficacy

FEBS Letters, EarlyView.
Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer, Matheus Felipe Santos, Maurício Mazzine Filho, Gabriela Oliveira Castro, Agustín Hernández, Manoel Aparecido Peres, Alejandro Miguel Katzin, Marcell Crispim   +7 more
wiley   +1 more source