FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Evaluation of Protein Complexes in Muscular Atrophy Using Interaction Map Analysis
Journal of Mazandaran University of Medical Sciences, 2019 Background and purpose: Muscular atrophy is a condition derived from different diseases and aging. Molecular study of the disease condition can help in developing diagnostic methods and treatment approaches. In this study, protein interaction network was
Mostafa Rezaei Tavirani +2 more
doaj
Reverse PCA, a systematic approach for identifying genes important for the physical interaction between protein pairs. [PDF]
PLoS Genetics, 2013 Protein-protein interactions (PPIs) are of central importance for many areas of biological research. Several complementary high-throughput technologies have been developed to study PPIs.
Ifat Lev +5 more
doaj +1 more source
X-ray Crystallographic Characterization of the Co(II)-substituted Tris-bound Form of the Aminopeptidase from \u3cem\u3eAeromonas proteolytica\u3c/em\u3e [PDF]
, 2007 The X-ray crystal structure of the Co(II)-loaded form of the aminopeptidase from Aeromonas proteolytica ([CoCo(AAP)]) was solved to 2.2 Å resolution. [CoCo(AAP)] folds into an α/β globular domain with a twisted β-sheet hydrophobic core sandwiched between
Bennett, Brian +6 more
core +1 more source
Statistical mechanics of nucleosome ordering by chromatin structure-induced two-body interactions
, 2011 One-dimensional arrays of nucleosomes (DNA-bound histone octamers separated by stretches of linker DNA) fold into higher-order chromatin structures which ultimately make up eukaryotic chromosomes.
Chereji, Răzvan V. +3 more
core +1 more source
Structural Evidence for a Copper-Bound Carbonate Intermediate in the Peroxidase and Dismutase Activities of Superoxide Dismutase [PDF]
, 2012 Copper-zinc superoxide dismutase (SOD) is of fundamental importance to our understanding of oxidative damage. Its primary function is catalysing the dismutation of superoxide to O2 and H2O2. SOD also reacts with H2O2, leading to the formation of a strong
Luis Eduardo Soares Netto +4 more
core +3 more sources
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
FEBS Letters, EarlyView.The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source
Prediction of C. elegans longevity genes by human and worm longevity networks. [PDF]
PLoS ONE, 2012 Intricate and interconnected pathways modulate longevity, but screens to identify the components of these pathways have not been saturating. Because biological processes are often executed by protein complexes and fine-tuned by regulatory factors, the ...
Robi Tacutu +6 more
doaj +1 more source
Intracellular protein interaction mapping with FRET hybrids [PDF]
Proceedings of the National Academy of Sciences, 2006 A quantitative methodology was developed to identify protein interactions in a broad range of cell types by using FRET between fluorescent proteins. Genetic fusions of a target receptor to a FRET acceptor and a large library of candidate peptide ligands to a FRET donor enabled high-throughput optical screening for optimal ...
Xia, You +5 more
openaire +2 more sources
Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?
FEBS Letters, EarlyView.This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes +3 more
wiley +1 more source