Results 61 to 70 of about 434,956 (254)

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

PEPpip: A Vision-Inspired Explainable Deep Learning Framework for Sequence-Based Protein–Protein Interaction Prediction and Interaction Map Estimation

IEEE Access
Predicting protein–protein interaction (PPI) patterns from primary sequences constitutes a complex pattern recognition task that demands advanced and explainable deep learning frameworks, as PPIs underpin critical cellular and pathological ...
Shubhrangshu Ghosh, Pralay Mitra
doaj   +1 more source

Prediction of C. elegans longevity genes by human and worm longevity networks. [PDF]

PLoS ONE, 2012
Intricate and interconnected pathways modulate longevity, but screens to identify the components of these pathways have not been saturating. Because biological processes are often executed by protein complexes and fine-tuned by regulatory factors, the ...
Robi Tacutu, David E Shore, Arie Budovsky, João Pedro de Magalhães, Gary Ruvkun, Vadim E Fraifeld, Sean P Curran   +6 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Mapping Dysfunctional Protein-Protein Interactions in Disease

Journal of Visualized Experiments
Protein-protein interaction (PPI) networks are dynamically remodeled in disease, yet most systems biology approaches focus on changes in protein abundance, overlooking critical interaction-level dysfunction. Here, we present a robust, chemoproteomic method-dysfunctional Protein-Protein Interactome (dfPPI)-that enables high-throughput, systematic ...
Rodina, Anna, Erdjument-Bromage, Hediye, Monetti, Mara, Li, Zhuoning, Chakrabarty, Souparna, Wang, Shujuan, Digwal, Chander S., Tuffery, Laura, Panchal, Palak, Sharma, Sahil, Roychowdhury, Tanaya, Neubert, Thomas A., Chiosis, Gabriela   +12 more
openaire   +2 more sources

New Molecular Aspects of Cardiac Arrest; Promoting Cardiopulmonary Resuscitation Approaches

Archives of Academic Emergency Medicine, 2018
Introduction: Cardiopulmonary resuscitation (CPR) is a method to improve survival of patients with cardiac arrest. This study aimed to identify the key genes affected five minutes after cardiac arrest, hoping to elevate the efficacy of CPR.
Mona Zamanian-Azodi, Mostafa Rezaei-Tavirani, Mohammad Rostami-Nejad, Fatemeh Tajik-Rostami   +3 more
doaj   +3 more sources

Differential network biology

Molecular Systems Biology, 2012
Protein and genetic interaction maps can reveal the overall physical and functional landscape of a biological system. To date, these interaction maps have typically been generated under a single condition, even though biological systems undergo ...
Trey Ideker, Nevan J Krogan
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source