Results 71 to 80 of about 1,139,517 (313)

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes [PDF]

, 2011
Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and
Delphine Cuchet-Lourenço, Roger D Everett, Boutell Chris, Roger D. Everett, Sykes Amanda, Murray Jill, Kyle Grant, Jill Murray, Cuchet-Lourenço Delphine, Chris Boutell, Sykes, A., Cuchet-Lourenço, D., Orr Anne, Grant, K., Everett, R.D., Grant Kyle, Boutell, C., Anne Orr, Amanda Sykes, Lukashchuk, V., Murray, J., Vera Lukashchuk, Everett Roger D., Lukashchuk Vera, Orr, A.   +24 more
core   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma

Journal of Experimental & Clinical Cancer Research, 2019
Background The first line therapy for patients with diffuse large B cell (DLBCL) is R-CHOP. About half of DLBCL patients are either refractory to, or will relapse, after the treatment.
Liling Jiang, Yuening Sun, Jinxiang Wang, Qingyan He, Xinmei Chen, Xiaoying Lan, Jinghong Chen, Q. Ping Dou, Xianping Shi, Jinbao Liu   +9 more
doaj   +1 more source

Site-selective protein modification by chemical design: towards multifunctional bioconjugates

, 2022
Proteins are considered as Nature’s most versatile and fascinating biomolecules due to their exceptional functional and structural properties. Molecular precision and defined geometry are the central hallmarks of proteins, which are considered as ...
Xu, Lujuan, Xu, L.
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Identification and functional characterization of the novel MAR-binding protein, SATB2 [PDF]

, 2004
The regulation of gene expression is governed in large part by transcription factors that bind to enhancers and promoters. The functions of transcription factors involve both the modulation of chromatin accessibility via the recruitment of histone ...
Dobreva, Gergana
core  

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Integrated machine learning identifies biomarkers for bilirubin-induced Alzheimer’s disease-like lesions in neonates and adults

Scientific Reports
Neurological impairments resulting from bilirubin encephalopathy represent a hallmark of bilirubin’s neurotoxic effects. Earlier research suggests that bilirubin may contribute to Alzheimer’s disease (AD) pathology by inducing neuronal necrosis and ...
Wenhao Bao, Han Chen, Zhuojun Zhang, Jiawen Wu, Jiaqian Lin, Daolin Tang, Jinbao Liu   +6 more
doaj   +1 more source