Results 41 to 50 of about 30,129 (222)

Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking [PDF]

, 2019
P2X receptor subunits (P2X1 to P2X7) assemble to form trimeric homomers or heteromers. Here, we describe the use of protein cross-linking to study the composition of P2X receptor complexes. This simple protocol is useful for determining the stoichiometry of P2X heteromeric receptors as well as for assessing the effect of point mutation, truncation, or ...
Vincent, Compan, François, Rassendren
openaire   +2 more sources

Pathogen infection and cholesterol deficiency activate the C. elegans p38 immune pathway through a TIR-1/SARM1 phase transition

eLife, 2022
Intracellular signaling regulators can be concentrated into membrane-free, higher ordered protein assemblies to initiate protective responses during stress — a process known as phase transition. Here, we show that a phase transition of the Caenorhabditis
Nicholas D Peterson, Janneke D Icso, J Elizabeth Salisbury, Tomás Rodríguez, Paul R Thompson, Read Pukkila-Worley   +5 more
doaj   +1 more source

TRIM5α and TRIMCyp form apparent hexamers and their multimeric state is not affected by exposure to restriction-sensitive viruses or by treatment with pharmacological inhibitors

Retrovirology, 2009
Proteins of the TRIM5 family, such as TRIM5α and the related TRIMCyp, are cytoplasmic factors that can inhibit incoming retroviruses. This type of restriction requires a direct interaction between TRIM5 proteins and capsid proteins that are part of ...
Berthoux Lionel, Bérubé Julie, Nepveu-Traversy Marie-Édith   +2 more
doaj   +1 more source

Cochlin, intraocular pressure regulation and mechanosensing. [PDF]

PLoS ONE, 2012
Fluid shear modulates many biological properties. How shear mechanosensing occurs in the extracellular matrix (ECM) and is transduced into cytoskeletal change remains unknown. Cochlin is an ECM protein of unknown function.
Manik Goel, Adam E Sienkiewicz, Renata Picciani, Jianhua Wang, Richard K Lee, Sanjoy K Bhattacharya   +5 more
doaj   +1 more source

Rapid Assembly of a Multimeric Membrane Protein Pore [PDF]

Biophysical Journal, 2011
We have observed the assembly of the staphylococcal pore-forming toxin α-hemolysin using single-molecule fluorescence imaging. Surprisingly, assembly from the monomer to the complete heptamer is extremely rapid, occurring in 
Thompson, James~R., Cronin, Bríd, Bayley, Hagan, Wallace, Mark Ian   +3 more
openaire   +3 more sources

TIA-1 Self-Multimerization, Phase Separation, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+

Cell Reports, 2018
Summary: Stress granules are non-membranous structures that transiently form in the cytoplasm during cellular stress, where they promote translational repression of non-essential RNAs and modulate cell signaling by sequestering key signal transduction ...
Joseph B. Rayman, Kevin A. Karl, Eric R. Kandel   +2 more
doaj   +1 more source

HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors

eLife, 2019
Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN.
Pratibha C Koneru, Ashwanth C Francis, Nanjie Deng, Stephanie V Rebensburg, Ashley C Hoyte, Jared Lindenberger, Daniel Adu-Ampratwum, Ross C Larue, Michael F Wempe, Alan N Engelman, Dmitry Lyumkis, James R Fuchs, Ronald M Levy, Gregory B Melikyan, Mamuka Kvaratskhelia   +14 more
doaj   +1 more source

Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase. [PDF]

PLoS Biology, 2016
The allosteric inhibitors of integrase (termed ALLINIs) interfere with HIV replication by binding to the viral-encoded integrase (IN) protein. Surprisingly, ALLINIs interfere not with DNA integration but with viral particle assembly late during HIV ...
Kushol Gupta, Vesa Turkki, Scott Sherrill-Mix, Young Hwang, Grant Eilers, Louis Taylor, Charlene McDanal, Ping Wang, David Temelkoff, Robert T Nolte, Emile Velthuisen, Jerry Jeffrey, Gregory D Van Duyne, Frederic D Bushman   +13 more
doaj   +1 more source

Structural basis for the multimerization of nonstructural protein nsp9 from SARS-CoV-2

Molecular Biomedicine, 2020
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of a potentially fatal disease named coronavirus disease 2019 (COVID-19), has raised significant public health concerns globally.
Changhui Zhang, Yiping Chen, Li Li, Yan Yang, Jun He, Cheng Chen, Dan Su   +6 more
doaj   +1 more source

Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coli

Journal of Genetic Engineering and Biotechnology, 2021
Background Difficult to express peptides are usually produced by co-expression with fusion partners. In this case, a significant mass part of the recombinant product falls on the subsequently removed fusion partner.
Murad Mollaev, Artur Zabolotskii, Neonila Gorokhovets, Elena Nikolskaya, Maria Sokol, Andrey Tsedilin, Mariia Mollaeva, Margarita Chirkina, Timofey Kuvaev, Anna Pshenichnikova, Nikita Yabbarov   +10 more
doaj   +1 more source