Results 61 to 70 of about 31,021 (196)

Trypanosoma brucei Acyl-Protein Thioesterase-like (TbAPT-L) Is a Lipase with Esterase Activity for Short and Medium-Chain Fatty Acids but Has No Depalmitoylation Activity

Pathogens, 2022
Dynamic post-translational modifications allow the rapid, specific, and tunable regulation of protein functions in eukaryotic cells. S-acylation is the only reversible lipid modification of proteins, in which a fatty acid, usually palmitate, is ...
Robert W. B. Brown, Aabha I. Sharma, Miguel Rey Villanueva, Xiaomo Li, Ouma Onguka, Leeor Zilbermintz, Helen Nguyen, Ben A. Falk, Cheryl L. Olson, Joann M. Taylor, Conrad L. Epting, Rahul S. Kathayat, Neri Amara, Bryan C. Dickinson, Matthew Bogyo, David M. Engman   +15 more
doaj   +1 more source

A Chemically Defined Four‐Component Self‐Adjuvanting Tn Vaccine Activating Mincle, FcγR, and CD206 for Enhanced Antitumor Immunity

Advanced Science, EarlyView.
A fully synthetic four‐component vaccine has been developed combining Tn antigen, vizantin (Mincle agonist), rhamnose (antibody recruitment), and mannose (CD206 targeting). By synergistically activating Mincle, FcγR, and CD206 pathways, it elicits strong IgG switching and cytotoxic T‐cells, significantly inhibiting tumors in mice.
Wenbo Ming, Renyu Zhang, Xiaohui Li, Guiqi Li, Yu Niu, Deying Yang, Xiang Luo, Jun Liao, Zhongqiu Liu, Guochao Liao   +9 more
wiley   +1 more source

Human Sialidase Neu3 is S-Acylated and Behaves Like an Integral Membrane Protein [PDF]

Scientific Reports, 2017
AbstractMembrane-bound sialidase Neu3 is involved in the catabolism of glycoconjugates, and plays crucial roles in numerous biological processes. Since the mechanism of its association with membranes is still not completely understood, the aim of this work was to provide further information regarding this aspect.
Macarena Rodríguez‐Walker, José L. Daniotti   +1 more
openaire   +4 more sources

Chemoselective Metabolomics via a Modular Reactivity‐Encoding Platform

Advanced Science, EarlyView.
Conventional LC–MS underrepresents numerous metabolites due to heterogeneous ionization efficiencies and matrix interference. Here, we introduce a modular reactivity‐encoding platform (MREP) comprising four alkyne‐tagged probes that selectively derivatize key functional groups, followed by unified click‐chemistry capture to eliminate matrix components ...
Xin Tao, Cang‐Man Zhang, Ru‐Jie Yang, Min Ren, Zhuo‐Ling Zhong, Kang‐Hui Wang, Rui‐Xue Hu, Jia‐Yue Liu, Jun‐Yi Zhou, Hou‐Kai Li, Jian‐Bo Wan   +10 more
wiley   +1 more source

Pseudo-enzymatic S-acylation of a myristoylated Yes protein tyrosine kinase peptide in vitro may reflect non-enzymatic S-acylation in vivo [PDF]

Biochemical Journal, 1998
Covalent attachment of a variety of lipid groups to proteins is now recognized as a major group of post-translational modifications. S-acylation of proteins at cysteine residues is the only modification considered dynamic and thus has the potential for regulating protein function and/or localization.
M C, Bañó, C S, Jackson, A I, Magee
openaire   +2 more sources

Salicylic acid attenuates brassinosteroid signaling via protein de‐S‐acylation

The EMBO Journal, 2023
Brassinosteroids (BRs) are important plant hormones involved in many aspects of development. Here, we show that BRASSINOSTEROID SIGNALING KINASEs (BSKs), key components of the BR pathway, are precisely controlled via de-S-acylation mediated by the defense hormone salicylic acid (SA).
Xiaoshi Liu, Zian Chen, Liting Huang, Youwei Ouyang, Zhiying Wang, Shuang Wu, Weixian Ye, Boya Yu, Yihang Zhang, Chengwei Yang, Jianbin Lai   +10 more
openaire   +2 more sources

S‐acylation of Ca²⁺ transport proteins in cancer

Chronic Diseases and Translational Medicine
AbstractAlterations in cellular calcium (Ca2+) signals have been causally associated with the development and progression of human cancers. Cellular Ca2+ signals are generated by channels, pumps, and exchangers that move Ca2+ ions across membranes and are decoded by effector proteins in the cytosol or in organelles. S‐acylation, the reversible addition
Kouba, Sana, Demaurex, Nicolas
openaire   +3 more sources

Protocol to identify S-acylated proteins in hippocampal neurons using ω-alkynyl fatty acid analogs and click chemistry

STAR Protocols
Summary: S-acylation, commonly palmitoylation, is the addition of fatty acids to cysteines to regulate protein localization and function. S-acylation detection has been hampered by limited sensitivity and selectivity in low-protein, costly samples like ...
Charlotte A. Townsend, Andrey A. Petropavlovskiy, Jordan A. Kogut, Alysha M. Church, Shaun S. Sanders   +4 more
doaj   +1 more source

Protein acylation in inflammatory diseases: from mechanisms to therapeutic strategies

Cell Communication and Signaling
Protein acylation, a critical subset of post-translational modifications (PTMs), serves as a dynamic regulatory mechanism linking cellular metabolism, epigenetic regulation, and inflammatory responses.
Jiayi Ding, Huiyi Wang, Zhengkun Yang, Xiaoxuan Wang, Zhengguo Cao   +4 more
doaj   +1 more source

The ankyrin repeats and DHHC S-acyl transferase domain of AKR1 act independently to regulate switching from vegetative to mating states in yeast. [PDF]

PLoS ONE, 2011
Signal transduction from G-protein coupled receptors to MAPK cascades through heterotrimeric G-proteins has been described for many eukaryotic systems.
Piers A Hemsley, Claire S Grierson
doaj   +1 more source