Results 1 to 10 of about 390,280 (339)
A cut above: atypical proteolysis endows complement C3 with non-canonical immune activities. [PDF]
EMBO JWest EE, Kemper C.
europepmc +2 more sources
The ATP/Mg2+ Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome
Methods and Protocols, 2022 Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg2+, and DTT.
Alexey Morozov +3 more
doaj +1 more source
Scientific Reports, 2022 Bacillus subtilis is a commensal member of the human oral and gut microbiomes, which can become infectious to immunocompromised patients. It possesses a conjugative transposon, ICEBs1, which includes > 20 genes and can be passed by horizontal gene ...
Rosanna Caliandro +2 more
doaj +1 more source
Frozen fresh blood plasma preserves the functionality of native human α2-macroglobulin
Scientific Reports, 2023 Human α2-macroglobulin (hα2M) is a large homotetrameric protein involved in the broad inhibition of endopeptidases. Following cleavage within a bait region, hα2M undergoes stepwise transitions from its native, expanded, highly flexible, active ...
Soraia R. Mendes +2 more
doaj +1 more source
Molecular and in vivo studies of a glutamate-class prolyl-endopeptidase for coeliac disease therapy
Nature Communications, 2022 Celiac disease is characterized by intolerance to gluten, a cereal protein. Here, the authors show that neprosin, a glutamate peptidase from the pitcher plant, efficiently cleaves gluten components under physiological conditions in vitro and in the gut ...
Laura del Amo-Maestro +10 more
doaj +1 more source
Structural and evolutionary insights into astacin metallopeptidases
Frontiers in Molecular Biosciences, 2023 The astacins are a family of metallopeptidases (MPs) that has been extensively described from animals. They are multidomain extracellular proteins, which have a conserved core architecture encompassing a signal peptide for secretion, a prodomain or ...
F. Xavier Gomis-Rüth, Walter Stöcker
doaj +1 more source
De novo design of immunoglobulin-like domains
Nature Communications, 2022 The immunoglobulin domain framework of antibodies has been a long standing design challenge. Here, the authors describe design rules for tailoring these domains and show they can be accurately designed, de novo, with high stability and the ability to ...
Tamuka M. Chidyausiku +13 more
doaj +1 more source
Product inhibition in native-state proteolysis. [PDF]
PLoS ONE, 2014 The proteolysis kinetics of intact proteins by nonspecific proteases provides valuable information on transient partial unfolding of proteins under native conditions.
Joseph R Kasper +2 more
doaj +1 more source
Cells, 2021 The degradation of most intracellular proteins is a dynamic and tightly regulated process performed by proteasomes. To date, different forms of proteasomes have been identified.
Alexander Burov +14 more
doaj +1 more source