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Towards a Quantitative Description of Proteolysis: Contribution of Demasking and Hydrolysis Steps to Proteolysis Kinetics of Milk Proteins [PDF]
FoodsThe hydrolysis of proteins by proteases (proteolysis) plays a significant role in biology and food science. Despite the importance of proteolysis, a universal quantitative model of this phenomenon has not yet been created.
Mikhail M. Vorob’ev
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The ATP/Mg2+ Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome
Methods and Protocols, 2022 Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg2+, and DTT.
Alexey Morozov +3 more
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Proteolysis-targeting chimeras (PROTACs) in cancer therapy
Molecular Cancer, 2022 Proteolysis-targeting chimeras (PROTACs) are engineered techniques for targeted protein degradation. A bifunctional PROTAC molecule with two covalently-linked ligands recruits target protein and E3 ubiquitin ligase together to trigger proteasomal ...
Xinyi Li +5 more
semanticscholar +1 more source
Scientific Reports, 2022 Bacillus subtilis is a commensal member of the human oral and gut microbiomes, which can become infectious to immunocompromised patients. It possesses a conjugative transposon, ICEBs1, which includes > 20 genes and can be passed by horizontal gene ...
Rosanna Caliandro +2 more
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Frozen fresh blood plasma preserves the functionality of native human α2-macroglobulin
Scientific Reports, 2023 Human α2-macroglobulin (hα2M) is a large homotetrameric protein involved in the broad inhibition of endopeptidases. Following cleavage within a bait region, hα2M undergoes stepwise transitions from its native, expanded, highly flexible, active ...
Soraia R. Mendes +2 more
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The Proteolysis of ECM in Intervertebral Disc Degeneration
International Journal of Molecular Sciences, 2022 Intervertebral disc (IVD) degeneration (IDD) is a pathological process that commonly occurs throughout the human life span and is a major cause of lower back pain. Better elucidation of the molecular mechanisms involved in disc degeneration could provide
Huaizhen Liang +5 more
semanticscholar +1 more source
Molecular and in vivo studies of a glutamate-class prolyl-endopeptidase for coeliac disease therapy
Nature Communications, 2022 Celiac disease is characterized by intolerance to gluten, a cereal protein. Here, the authors show that neprosin, a glutamate peptidase from the pitcher plant, efficiently cleaves gluten components under physiological conditions in vitro and in the gut ...
Laura del Amo-Maestro +10 more
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Structural and evolutionary insights into astacin metallopeptidases
Frontiers in Molecular Biosciences, 2023 The astacins are a family of metallopeptidases (MPs) that has been extensively described from animals. They are multidomain extracellular proteins, which have a conserved core architecture encompassing a signal peptide for secretion, a prodomain or ...
F. Xavier Gomis-Rüth, Walter Stöcker
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De novo design of immunoglobulin-like domains
Nature Communications, 2022 The immunoglobulin domain framework of antibodies has been a long standing design challenge. Here, the authors describe design rules for tailoring these domains and show they can be accurately designed, de novo, with high stability and the ability to ...
Tamuka M. Chidyausiku +13 more
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