Results 1 to 10 of about 195,001 (261)

Oxidized/deamidated-ceruloplasmin dysregulates choroid plexus epithelial cells functionality and barrier properties via RGD-recognizing integrin binding

Neurobiology of Disease, 2021
Choroid plexus epithelial cells (CPEpiCs) determine the composition of cerebrospinal fluid (CSF) and constitute the blood-CSF barrier (BCSFB), functions that are altered in neurodegenerative diseases.
Alan Zanardi, Marco Barbariga, Antonio Conti, Franco Vegliani, Flavio Curnis, Massimo Alessio   +5 more
doaj   +1 more source

Dataset containing physiological amounts of spike-in proteins into murine C2C12 background as a ground truth quantitative LC-MS/MS reference

Data in Brief, 2022
In this article, we present a data dependent acquisition (DDA) dataset which was generated as a reference and ground truth quantitative dataset. While initially used to compare samples measured with DDA and data independent acquisition (DIA) (Barkovits ...
Julian Uszkoreit, Katalin Barkovits, Sandra Pacharra, Kathy Pfeiffer, Simone Steinbach, Katrin Marcus, Martin Eisenacher   +6 more
doaj   +1 more source

Quality Control—A Stepchild in Quantitative Proteomics: A Case Study for the Human CSF Proteome

Biomolecules, 2023
Proteomic studies using mass spectrometry (MS)-based quantification are a main approach to the discovery of new biomarkers. However, a number of analytical conditions in front and during MS data acquisition can affect the accuracy of the obtained outcome.
Svitlana Rozanova, Julian Uszkoreit, Karin Schork, Bettina Serschnitzki, Martin Eisenacher, Lars Tönges, Katalin Barkovits-Boeddinghaus, Katrin Marcus   +7 more
doaj   +1 more source

Intact Transition Epitope Mapping—Serological Inspection by Epitope EXtraction (ITEM—SIX)

Molecules, 2023
Precision medicine requests accurate serological inspections to precisely stratify patients for targeted treatment. Intact transition epitope mapping analysis proved surrogate seroconversion of a model organism’s serum when spiked with a monoclonal ...
Agatino Zammataro, Cornelia Koy, Manuela Ruß, Claudia Röwer, Michael O. Glocker   +4 more
doaj   +1 more source

Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization

Molecules, 2020
Electrospray mass spectrometry is applied to determine apparent binding energies and quasi equilibrium dissociation constants of immune complex dissociation reactions in the gas phase. Myoglobin, a natural protein-ligand complex, has been used to develop
Bright D. Danquah, Kwabena F. M. Opuni, Claudia Roewer, Cornelia Koy, Michael O. Glocker   +4 more
doaj   +1 more source

An Ultrafast N-Glycoproteome Analysis Method Using Thermoresponsive Magnetic Fluid-Immobilized Enzymes

Frontiers in Chemistry, 2021
N-Glycosylation is one of the most common and important post-translational modification methods, and it plays a vital role in controlling many biological processes.
Zhiya Fan, Tong Liu, Fei Zheng, Weijie Qin, Weijie Qin, Xiaohong Qian   +5 more
doaj   +1 more source

Tidyproteomics: an open-source R package and data object for quantitative proteomics post analysis and visualization

BMC Bioinformatics, 2023
Background The analysis of mass spectrometry-based quantitative proteomics data can be challenging given the variety of established analysis platforms, the differences in reporting formats, and a general lack of approachable standardized post-processing ...
Jeff Jones, Elliot J. MacKrell, Ting-Yu Wang, Brett Lomenick, Michael L. Roukes, Tsui-Fen Chou   +5 more
doaj   +1 more source

Characterization of the E.coli proteome and its modifications during growth and ethanol stress

Frontiers in Microbiology, 2015
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational
Boumediene eSoufi, Karsten eKrug, Andreas eHarst, Boris eMacek   +3 more
doaj   +1 more source

Prion-like Domains in Spike Protein of SARS-CoV-2 Differ across Its Variants and Enable Changes in Affinity to ACE2

Microorganisms, 2022
Currently, the world is struggling with the coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
George Tetz, Victor Tetz
doaj   +1 more source

RNA sequencing and transcriptome arrays analyses show opposing results for alternative splicing in patient derived samples

BMC Genomics, 2017
Background RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms.
Petr V. Nazarov, Arnaud Muller, Tony Kaoma, Nathalie Nicot, Cristina Maximo, Philippe Birembaut, Nhan L. Tran, Gunnar Dittmar, Laurent Vallar   +8 more
doaj   +1 more source