Results 11 to 20 of about 461,354 (348)

Is a gene-centric human proteome project the best way for proteomics to serve biology? [PDF]

Proteomics (2010) epub ahead of print, 2010
With the recent developments in proteomic technologies, a complete human proteome project (HPP) appears feasible for the first time. However, there is still debate as to how it should be designed and what it should encompass.
A gene-centric Human Proteome project, Addona, Aicher, Andersen, Anderson, Anderson, Anderson, Baker, Berglund, Burckstummer, Butkinaree, Charrier, Cheng, Christensen, Deutsch, Foster, Fusaro, Gavin, Golub, Graumann, Hochstrasser, Ivanov, John, Kuhn, Lange, Lasonder, Lescuyer, Lu, Mallick, Maltese, Mathivanan, Pearson, Picotti, Picotti, Rigaut, Rout, Sato, Seo, Service, Taylor, Trinkle-Mulcahy, Unwin, Venter, Wang, Washburn, Weinberg, Whiteaker, Willumsen, Yang   +48 more
core   +6 more sources

Organelle Proteomics [PDF]

Current Protocols in Protein Science, 2006
AbstractThis unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided.
Lescuyer, Pierre, Chevallet, Mireille, Luche, Sylvie, Rabilloud, Thierry   +3 more
openaire   +7 more sources

Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg [PDF]

PROTEOMICS 13, 20 (2013) 2942-3, 2013
While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC ...
Mayr, Manuel, Rabilloud, Thierry
core   +5 more sources

Highly accurate protein structure prediction for the human proteome

Nature, 2021
Protein structures can provide invaluable information, both for reasoning about biological processes and for enabling interventions such as structure-based drug development or targeted mutagenesis.
Kathryn Tunyasuvunakool, J. Adler, Zachary Wu, Tim Green, Michal Zielinski, Augustin Žídek, Alex Bridgland, A. Cowie, Clemens Meyer, Agata Laydon, S. Velankar, G. Kleywegt, A. Bateman, R. Evans, A. Pritzel, Michael Figurnov, O. Ronneberger, Russ Bates, Simon A A Kohl, Anna Potapenko, A. J. Ballard, Bernardino Romera-Paredes, Stanislav Nikolov, Rishub Jain, Ellen Clancy, D. Reiman, Stig Petersen, A. Senior, K. Kavukcuoglu, E. Birney, Pushmeet Kohli, J. Jumper, D. Hassabis   +32 more
semanticscholar   +1 more source

Accurate proteome-wide missense variant effect prediction with AlphaMissense

Science, 2023
The vast majority of missense variants observed in the human genome are of unknown clinical significance. We present AlphaMissense, an adaptation of AlphaFold fine-tuned on human and primate variant population frequency databases to predict missense ...
Jun Cheng, Guido Novati, Joshua Pan, Clare Bycroft, Akvilė Žemgulytė, Taylor Applebaum, A. Pritzel, L. H. Wong, Michal Zielinski, Tobias Sargeant, Rosalia G. Schneider, Andrew Senior, J. Jumper, D. Hassabis, Pushmeet Kohli, Žiga Avsec   +15 more
semanticscholar   +1 more source

Quality Control—A Stepchild in Quantitative Proteomics: A Case Study for the Human CSF Proteome

Biomolecules, 2023
Proteomic studies using mass spectrometry (MS)-based quantification are a main approach to the discovery of new biomarkers. However, a number of analytical conditions in front and during MS data acquisition can affect the accuracy of the obtained outcome.
Svitlana Rozanova, Julian Uszkoreit, Karin Schork, Bettina Serschnitzki, Martin Eisenacher, Lars Tönges, Katalin Barkovits-Boeddinghaus, Katrin Marcus   +7 more
doaj   +1 more source

Intact Transition Epitope Mapping—Serological Inspection by Epitope EXtraction (ITEM—SIX)

Molecules, 2023
Precision medicine requests accurate serological inspections to precisely stratify patients for targeted treatment. Intact transition epitope mapping analysis proved surrogate seroconversion of a model organism’s serum when spiked with a monoclonal ...
Agatino Zammataro, Cornelia Koy, Manuela Ruß, Claudia Röwer, Michael O. Glocker   +4 more
doaj   +1 more source

Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization

Molecules, 2020
Electrospray mass spectrometry is applied to determine apparent binding energies and quasi equilibrium dissociation constants of immune complex dissociation reactions in the gas phase. Myoglobin, a natural protein-ligand complex, has been used to develop
Bright D. Danquah, Kwabena F. M. Opuni, Claudia Roewer, Cornelia Koy, Michael O. Glocker   +4 more
doaj   +1 more source

Tidyproteomics: an open-source R package and data object for quantitative proteomics post analysis and visualization

BMC Bioinformatics, 2023
Background The analysis of mass spectrometry-based quantitative proteomics data can be challenging given the variety of established analysis platforms, the differences in reporting formats, and a general lack of approachable standardized post-processing ...
Jeff Jones, Elliot J. MacKrell, Ting-Yu Wang, Brett Lomenick, Michael L. Roukes, Tsui-Fen Chou   +5 more
doaj   +1 more source

An Ultrafast N-Glycoproteome Analysis Method Using Thermoresponsive Magnetic Fluid-Immobilized Enzymes

Frontiers in Chemistry, 2021
N-Glycosylation is one of the most common and important post-translational modification methods, and it plays a vital role in controlling many biological processes.
Zhiya Fan, Tong Liu, Fei Zheng, Weijie Qin, Weijie Qin, Xiaohong Qian   +5 more
doaj   +1 more source