Results 11 to 20 of about 205,179 (309)
Curr Protoc Protein Sci Chapter 24 (2006) Unit 24.2, 2009 This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided.
Lescuyer, Pierre +3 more
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Journal of Education, Health and Sport, 2021 Proteomics is a branch of molecular biology that deals with the identification and quantification of proteins in living objects, as well as the analysis of protein functions and their interactions. Proteomics is studied by proteins that are expressed in a given cell, tissue or organism over a period of time (under certain conditions). It is
Dolomatov, S.I. +2 more
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Proteomes, 2020 Brucella spp. are Gram negative intracellular bacteria responsible for brucellosis, a worldwide distributed zoonosis. A prominent aspect of the Brucella life cycle is its ability to invade, survive and multiply within host cells. Comprehensive approaches, such as proteomics, have aided in unravelling the molecular mechanisms underlying Brucella ...
Ansgar Poetsch +2 more
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Nature Reviews Methods Primers, 2021 The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchrony. Dynamic movement of proteins between niches is essential for cellular processes such as signalling, growth ...
Josie A. Christopher +14 more
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Frontiers in Microbiology, 2019 Lipopolysaccharides (LPS) in the outer membrane of Gram-negative bacteria provide the first line of defense against antibiotics and other harmful compounds. LPS biosynthesis critically depends on LpxC catalyzing the first committed enzyme in this process.
Nikolas Thomanek +7 more
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How to use 2D gel electrophoresis in plant proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 1072 (2014)
43-50, 2013 Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv +1 more source
Neurobiology of Disease, 2021 Choroid plexus epithelial cells (CPEpiCs) determine the composition of cerebrospinal fluid (CSF) and constitute the blood-CSF barrier (BCSFB), functions that are altered in neurodegenerative diseases.
Alan Zanardi +5 more
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Proteomes: A New Proteomic Journal [PDF]
Proteomes, 2012 In the early years of proteomics, mass spectrometry served only as a technique in protein chemistry facilitating the characterization of purified proteins and mapping their posttranslational modifications (PTMs). A bit later this technique almost completely replaced Edman degradation and amino acid analysis.
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Predicting clinical outcome of neuroblastoma patients using an integrative network-based approach
Biology Direct, 2018 Background One of the main current challenges in computational biology is to make sense of the huge amounts of multidimensional experimental data that are being produced.
Léon-Charles Tranchevent +7 more
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BMC Molecular and Cell Biology, 2021 Background One striking feature of the large KRAB domain-containing zinc finger protein (KZFP) family is its rapid evolution, leading to hundreds of member genes with various origination time in a certain mammalian genome. However, a comprehensive genome-
Pan Shen +6 more
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