Results 21 to 30 of about 461,354 (348)

Characterization of the E.coli proteome and its modifications during growth and ethanol stress

Frontiers in Microbiology, 2015
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational
Boumediene eSoufi, Karsten eKrug, Andreas eHarst, Boris eMacek   +3 more
doaj   +1 more source

Prion-like Domains in Spike Protein of SARS-CoV-2 Differ across Its Variants and Enable Changes in Affinity to ACE2

Microorganisms, 2022
Currently, the world is struggling with the coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
George Tetz, Victor Tetz
doaj   +1 more source

Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ *

Molecular & Cellular Proteomics, 2014
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination
J. Cox, Marco Y. Hein, Christian A. Luber, Igor Paron, Nagarjuna Nagaraj, M. Mann   +5 more
semanticscholar   +1 more source

Recent advances in CRISPR-based genome editing technology and its applications in cardiovascular research

Military Medical Research, 2023
The rapid development of genome editing technology has brought major breakthroughs in the fields of life science and medicine. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing toolbox has been ...
Zhen-Hua Li, Jun Wang, Jing-Ping Xu, Jian Wang, Xiao Yang   +4 more
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Sampling the proteome by emerging single-molecule and mass-spectrometry methods [PDF]

Nat Methods 20, 339--346 (2023), 2022
Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules. This larger number of molecules and the associated larger dynamic range have major implications in the development of proteomics technologies. We examine these implications for both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and single-molecule counting ...
arxiv   +1 more source

RNA sequencing and transcriptome arrays analyses show opposing results for alternative splicing in patient derived samples

BMC Genomics, 2017
Background RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms.
Petr V. Nazarov, Arnaud Muller, Tony Kaoma, Nathalie Nicot, Cristina Maximo, Philippe Birembaut, Nhan L. Tran, Gunnar Dittmar, Laurent Vallar   +8 more
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MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations

Nucleic Acids Res., 2020
The mammalian mitochondrial proteome is under dual genomic control, with 99% of proteins encoded by the nuclear genome and 13 originating from the mitochondrial DNA (mtDNA).
S. Rath, Rohit Sharma, Rahul Gupta, Tslil Ast, Connie Chan, Timothy J. Durham, Russell P. Goodman, Z. Grabarek, Mary E. Haas, Wendy W K Hung, Pallavi R. Joshi, Alexis A. Jourdain, S. H. Kim, Anna V. Kotrys, Stephanie S. Lam, J. McCoy, Joshua D. Meisel, Maria Miranda, Apekshya Panda, Anupam Patgiri, Robert S Rogers, Shayan Sadre, Hardik Shah, Owen S. Skinner, Tsz-Leung To, M. Walker, Hong Wang, P. Ward, Jordan Wengrod, Chen-Ching Yuan, S. Calvo, V. Mootha   +31 more
semanticscholar   +1 more source

Intricate Crosstalk Between Lipopolysaccharide, Phospholipid and Fatty Acid Metabolism in Escherichia coli Modulates Proteolysis of LpxC

Frontiers in Microbiology, 2019
Lipopolysaccharides (LPS) in the outer membrane of Gram-negative bacteria provide the first line of defense against antibiotics and other harmful compounds. LPS biosynthesis critically depends on LpxC catalyzing the first committed enzyme in this process.
Nikolas Thomanek, Jan Arends, Claudia Lindemann, Katalin Barkovits, Helmut E. Meyer, Helmut E. Meyer, Katrin Marcus, Franz Narberhaus   +7 more
doaj   +1 more source

How to use 2D gel electrophoresis in plant proteomics [PDF]

Methods in Molecular Biology -Clifton then Totowa- 1072 (2014) 43-50, 2013
Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv   +1 more source

Oxidized/deamidated-ceruloplasmin dysregulates choroid plexus epithelial cells functionality and barrier properties via RGD-recognizing integrin binding

Neurobiology of Disease, 2021
Choroid plexus epithelial cells (CPEpiCs) determine the composition of cerebrospinal fluid (CSF) and constitute the blood-CSF barrier (BCSFB), functions that are altered in neurodegenerative diseases.
Alan Zanardi, Marco Barbariga, Antonio Conti, Franco Vegliani, Flavio Curnis, Massimo Alessio   +5 more
doaj