Results 21 to 30 of about 205,179 (309)
In silico Proteome Cleavage Reveals Iterative Digestion Strategy for High Sequence Coverage [PDF]
ISRN Computational Biology 2014, 2014 In the post-genome era, biologists have sought to measure the complete complement of proteins, termed proteomics. Currently, the most effective method to measure the proteome is with shotgun, or bottom-up, proteomics, in which the proteome is digested into peptides that are identified followed by protein inference.
arxiv +1 more source
Data in Brief, 2022 In this article, we present a data dependent acquisition (DDA) dataset which was generated as a reference and ground truth quantitative dataset. While initially used to compare samples measured with DDA and data independent acquisition (DIA) (Barkovits ...
Julian Uszkoreit +6 more
doaj
Proteomic overview of hepatocellular carcinoma cell lines and generation of the spectral library
Scientific Data, 2022 Measurement(s) Proteome of hepatocellular carcinoma cell lines Technology Type(s) Liquid chromatography-tandem mass spectrometry Sample Characteristic - Organism Homo ...
Mingchao Wang +6 more
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Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv +1 more source
proteomics technologies: Probing the proteome [PDF]
Nature, 2003 The completion of the human genome sequence, coupled with analytical techniques such as mass spectrometry, has fuelled interest in proteomics. Diane Gershon reports.
openaire +3 more sources
Cell Death and Disease, 2023 BRISC (BRCC3 isopeptidase complex) is a deubiquitinating enzyme that has been linked with inflammatory processes, but its role in liver diseases and the underlying mechanism are unknown. Here, we investigated the pathophysiological role of BRISC in acute
Wen Zhang +20 more
doaj +1 more source
Is a gene-centric human proteome project the best way for proteomics to serve biology? [PDF]
Proteomics (2010) epub ahead of print, 2010 With the recent developments in proteomic technologies, a complete human proteome project (HPP) appears feasible for the first time. However, there is still debate as to how it should be designed and what it should encompass. In "proteomics speak", the debate revolves around the central question as to whether a gene-centric or a protein-centric ...
arxiv +1 more source
Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation [PDF]
Genome Biology, 2018, 2018 Cellular heterogeneity is important to biological processes, including cancer and development. However, proteome heterogeneity is largely unexplored because of the limitations of existing methods for quantifying protein levels in single cells. To alleviate these limitations, we developed Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS), and ...
arxiv +1 more source
High-affinity SOAT1 ligands remodeled cholesterol metabolism program to inhibit tumor growth
BMC Medicine, 2022 Background Although cholesterol metabolism is a common pathway for the development of antitumor drugs, there are no specific targets and drugs for clinical use.
Zhihua Wang +10 more
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Progress in the definition of a reference human mitochondrial proteome [PDF]
Proteomics 3 (02/2003) 157-67, 2006 Owing to the complexity of higher eukaryotic cells, a complete proteome is likely to be very difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized tools to study specifically the biology and "physiology" of the target organelle. Within this frame,
arxiv +1 more source