Results 21 to 30 of about 538,471 (385)

Adaptations of Escherichia coli strains to oxidative stress are reflected in properties of their structural proteomes. [PDF]

, 2020
BACKGROUND:The reconstruction of metabolic networks and the three-dimensional coverage of protein structures have reached the genome-scale in the widely studied Escherichia coli K-12 MG1655 strain.
Catoiu, Edward, Fang, Xin, Heckmann, David, Mih, Nathan, Monk, Jonathan M, Palsson, Bernhard O, Yang, Laurence   +6 more
core   +2 more sources

Positional proteomics reveals differences in N-terminal proteoform stability [PDF]

, 2016
To understand the impact of alternative translation initiation on a proteome, we performed a proteome-wide study on protein turnover using positional proteomics and ribosome profiling to distinguish between N-terminal proteoforms of individual genes.
Brown JL, Daria Gawron, Elvis Ndah, Kris Gevaert, Petra Van Damme   +4 more
core   +2 more sources

Recent advances in CRISPR-based genome editing technology and its applications in cardiovascular research

Military Medical Research, 2023
The rapid development of genome editing technology has brought major breakthroughs in the fields of life science and medicine. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing toolbox has been ...
Zhen-Hua Li, Jun Wang, Jing-Ping Xu, Jian Wang, Xiao Yang   +4 more
doaj   +1 more source

Pharmacoproteomic characterisation of human colon and rectal cancer [PDF]

, 2017
Most molecular cancer therapies act on protein targets but data on the proteome status of patients and cellular models for proteome-guided pre-clinical drug sensitivity studies are only beginning to emerge.
Domingo, Enric, Feller, Stephan M, Frejno, Martin, Heinzlmeir, Stephanie, Jarzab, Anna, Jesinghaus, Moritz, Johnstone, Elaine, Kerr, David, Klaeger, Susan, Knapp, Stefan, Koch, Heiner, Kramer, Karl, Kuster, Bernhard, Meng, Chen, Ruprecht, Benjamin, Slotta-Huspenina, Julia, Weichert, Wilko, Wilhelm, Mathias, Zenezini Chiozzi, Riccardo, Zheng, Runsheng   +19 more
core   +1 more source

RNA sequencing and transcriptome arrays analyses show opposing results for alternative splicing in patient derived samples

BMC Genomics, 2017
Background RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms.
Petr V. Nazarov, Arnaud Muller, Tony Kaoma, Nathalie Nicot, Cristina Maximo, Philippe Birembaut, Nhan L. Tran, Gunnar Dittmar, Laurent Vallar   +8 more
doaj   +1 more source

DART-ID increases single-cell proteome coverage. [PDF]

, 2019
Analysis by liquid chromatography and tandem mass spectrometry (LC-MS/MS) can identify and quantify thousands of proteins in microgram-level samples, such as those comprised of thousands of cells.
Chen, Albert Tian, Franks, Alexander, Slavov, Nikolai   +2 more
core   +3 more sources

Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ *

Molecular & Cellular Proteomics, 2014
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination
J. Cox, Marco Y. Hein, Christian A. Luber, Igor Paron, Nagarjuna Nagaraj, M. Mann   +5 more
semanticscholar   +1 more source

Intricate Crosstalk Between Lipopolysaccharide, Phospholipid and Fatty Acid Metabolism in Escherichia coli Modulates Proteolysis of LpxC

Frontiers in Microbiology, 2019
Lipopolysaccharides (LPS) in the outer membrane of Gram-negative bacteria provide the first line of defense against antibiotics and other harmful compounds. LPS biosynthesis critically depends on LpxC catalyzing the first committed enzyme in this process.
Nikolas Thomanek, Jan Arends, Claudia Lindemann, Katalin Barkovits, Helmut E. Meyer, Helmut E. Meyer, Katrin Marcus, Franz Narberhaus   +7 more
doaj   +1 more source

Sex-partitioning of the Plasmodium falciparum stage V gametocyte proteome provides insight into falciparum-specific cell biology [PDF]

, 2014
One of the critical gaps in malaria transmission biology and surveillance is our lack of knowledge about Plasmodium falciparum gametocyte biology, especially sexual dimorphic development and how sex ratios that may influence transmission from the human ...
Dinglasan, R. R., Goldowitz, I., Graham, D. R., King, J. G., Marti, M., Mathias, D. K., Moss, E., Pastrana-Mena, R., Tao, D., Tripathi, A., Ubaida-Mohien, C.   +10 more
core   +1 more source

Organelle Proteomics [PDF]

Current Protocols in Protein Science, 2006
AbstractThis unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided.
Lescuyer, Pierre, Chevallet, Mireille, Luche, Sylvie, Rabilloud, Thierry   +3 more
openaire   +5 more sources