Results 141 to 150 of about 719 (174)

Label-Free Multiplex Proteotyping of Microbial Isolates

Analytical Chemistry, 2023
To meet clinical diagnostic needs and for general microbiological screening, it is essential to be able to accurately and rapidly identify any microorganisms from complex microbiota. To gain insight into the individual components of microbiota, culturomics has been proposed as a means to systematically test hundreds of possible cultivation conditions ...
Chabas, Madisson, Pible, Olivier, Armengaud, Jean, Alpha-Bazin, Béatrice   +3 more
openaire   +4 more sources

Differentiation of Campylobacter fetus subspecies by proteotyping [PDF]

European Journal of Microbiology and Immunology, 2019
Campylobacter fetus is a causative agent of intestinal illness and, occasionally, severe systemic infections and meningitis. C. fetus currently comprises three subspecies: C. fetus subspecies fetus (Cff), C. fetus subspecies venerealis (Cfv), and C. fetus subspecies testudinum (Cft).
Matthias Emele, Helmut Hotzel, Uwe Groß
exaly   +6 more sources

Variant mapping using mass spectrometry–based proteotyping as a diagnostic tool in von Willebrand disease [PDF]

Journal of Thrombosis and Haemostasis
Background: von Willebrand disease (VWD) is the most common inherited bleeding disorder, characterized by either partial or complete von Willebrand factor (VWF) deficiency or by the occurrence of VWF proteoforms of altered functionality.
Ferdows Atiq, Ruben Bierings, Maartje van den Biggelaar   +2 more
exaly   +7 more sources

Proteotyping pluripotency with mass spectrometry

Expert Review of Proteomics, 2019
Pluripotency emerges transiently during embryogenesis in two main forms with different developmental potential, termed naïve and primed states. Importantly, these pluripotent states can be recapitulated in vitro under specific culture conditions, representing a unique model to study the regulatory principles of development and cellular plasticity ...
Cristina, Sayago, Ana, Martinez-Val, Javier, Munoz   +2 more
openaire   +2 more sources

Computational prediction of proteotypic peptides

Expert Review of Proteomics, 2007
Evaluation of: Mallick P, Schirle M, Chen SS et al. Computational prediction of proteotypic peptides for quantitative proteomics. Nat. Biotechnol. 25(1), 125–131 (2007).Mass spectrometry, the driving analytical force behind proteomics, is primarily used to identify and quantify as many proteins in a complex biological mixture as possible.
Josip, Blonder, Timothy D, Veenstra
openaire   +2 more sources

Scoring proteomes with proteotypic peptide probes

Nature Reviews Molecular Cell Biology, 2005
Technologies for genome-wide analyses typically undergo a transition from a discovery phase to a scoring phase. In the discovery phase, the genomic universe is explored and all pertinent data are noted. In the scoring phase, relevant entities are screened to reveal groups of genes that are associated with specific biological processes or conditions. In
Bernhard, Kuster, Markus, Schirle, Parag, Mallick, Ruedi, Aebersold   +3 more
openaire   +2 more sources

Proteotypic Peptides and Their Applications

2016
Recent advances in mass spectrometry based proteomic techniques and publicly available large proteomic repositories are being exploited to characterize the proteome of multiple organisms. While humongous amount of proteomic data is being acquired and analyzed, many biological questions still remain unanswered.
Shivakumar, Keerthikumar, Suresh, Mathivanan   +1 more
openaire   +2 more sources

Alpha-1-Antitrypsin (A1AT) Proteotyping by LC-MS/MS

2023
The diagnosis of alpha-1-antitrypsin (A1AT) deficiency is established by quantitation of protein concentration in serum (immunoassay) followed by determination of specific allelic variants by phenotyping (isoelectric focusing (IEF) gel electrophoresis) and/or allele-specific genotyping.
Jennifer, Kemp, Paula M, Ladwig, Melissa R, Snyder   +2 more
openaire   +2 more sources

Proteotype Co-evolution and Diversity in Mammals

2022
AbstractEvolutionary profiling has been largely limited to the nucleotide level. Using consistent proteomic methods, we quantified proteomic and phosphoproteomic layers in fibroblasts from 11 common mammalian species, with transcriptomic variability as reference.
Qian Ba, Yuanyuan Hei, Anasuya Dighe, Wenxue Li, Jamie Maziarz, Irene Pak, Shisheng Wang, Günter P. Wagner, Yansheng Liu   +8 more
openaire   +1 more source

Computational prediction of proteotypic peptides for quantitative proteomics

Nature Biotechnology, 2006
Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified for any given protein present in a ...
Parag, Mallick, Markus, Schirle, Sharon S, Chen, Mark R, Flory, Hookeun, Lee, Daniel, Martin, Jeffrey, Ranish, Brian, Raught, Robert, Schmitt, Thilo, Werner, Bernhard, Kuster, Ruedi, Aebersold   +11 more
openaire   +2 more sources