Results 131 to 140 of about 507 (153)

The use of proteotypic peptide libraries for protein identification

Rapid Communications in Mass Spectrometry, 2005
Abstract This paper describes an algorithm to apply proteotypic peptide sequence libraries to protein identifications performed using tandem mass spectrometry (MS/MS). Proteotypic peptides are those peptides in a protein sequence that are most likely to be confidently observed by current MS‐based proteomics ...
Robertson, Craig, John P, Cortens, Ronald C, Beavis   +2 more
openaire   +2 more sources

Proteotype Co-evolution and Diversity in Mammals

2022
Abstract Evolutionary profiling has been largely limited to the nucleotide level. Using consistent proteomic methods, we quantified proteomic and phosphoproteomic layers in fibroblasts from 11 common mammalian species, with transcriptomic variability as reference.
Qian Ba, Yuanyuan Hei, Anasuya Dighe, Wenxue Li, Jamie Maziarz, Irene Pak, Shisheng Wang, Günter P. Wagner, Yansheng Liu   +8 more
openaire   +1 more source

Computational prediction of proteotypic peptides for quantitative proteomics

Nature Biotechnology, 2006
Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified for any given protein present in a ...
Parag, Mallick, Markus, Schirle, Sharon S, Chen, Mark R, Flory, Hookeun, Lee, Daniel, Martin, Jeffrey, Ranish, Brian, Raught, Robert, Schmitt, Thilo, Werner, Bernhard, Kuster, Ruedi, Aebersold   +11 more
openaire   +2 more sources

Proteotyping of Mammary Tissue from Transgenic and Gene Knockout Mice with Immunohistochemical Markers: a Tool To Define Developmental Lesions

Journal of Histochemistry and Cytochemistry, 2003
Through the use of transgenic and gene knockout mice, several studies have identified specific genes required for the functional development of mammary epithelium.
Jonathan M Shillingford, Keiko Miyoshi, Gertraud W Robinson   +2 more
exaly   +2 more sources

LineageFilter: Improved Proteotyping of Complex Samples Using Metaproteomics and Machine Learning

Journal of Proteome Research
International audienceMetaproteomics is a powerful tool to characterize how microbiota function by analyzing their proteic content by tandem mass spectrometry.
Jean Armengaud, Lucia Grenga, Olivier Pible   +2 more
exaly   +2 more sources

Alpha-1-Antitrypsin (A1AT) Proteotyping by LC-MS/MS

2023
The diagnosis of alpha-1-antitrypsin (A1AT) deficiency is established by quantitation of protein concentration in serum (immunoassay) followed by determination of specific allelic variants by phenotyping (isoelectric focusing (IEF) gel electrophoresis) and/or allele-specific genotyping.
Jennifer, Kemp, Paula M, Ladwig, Melissa R, Snyder   +2 more
openaire   +2 more sources

Proteotyping of the Parainfluenza Virus with High-Resolution Mass Spectrometry

Analytical Chemistry, 2013
Parainfluenza viruses (PIVs) are one of the most common causes of respiratory tract infections in children and can be life-threatening when the airway becomes obstructed. Infection results in a spectrum of respiratory disease symptoms that makes diagnosis difficult.
An P, Nguyen, Kevin M, Downard
openaire   +2 more sources

Flash MS/MS proteotyping allows identifying microbial isolates in 36 s of mass spectrometry signal

Proteomics
International audienceAbstract Rapid identification of microorganisms is essential for medical diagnostics, sanitary controls, and food safety. High‐throughput analytical platforms currently rely on whole‐cell MALDI‐TOF mass spectrometry to process ...
Jean-Charles Gaillard, Beatrice Alpha-Bazin, Jean Armengaud   +2 more
exaly   +2 more sources

Elucidation of host-virus surfaceome interactions using spatial proteotyping

2021
The cellular surfaceome and its residing extracellularly exposed proteins are involved in a multitude of molecular signaling processes across the viral infection cycle. Successful viral propagation, including viral entry, immune evasion, virion release and viral spread rely on dynamic molecular interactions with the surfaceome.
Fabian, Wendt, Emanuela S, Milani, Bernd, Wollscheid   +2 more
openaire   +2 more sources

Proteotyping Gene Dosage Effects in Genetic Diseases

The FASEB Journal, 2017
Introduction and Objectives Gene dosage imbalance is a general working hypothesis for studying the genetic diseases, such as human syndromes, because the DNA copy number variations (CNVs) are causal for many genetic diseases.
Yansheng Liu, Ruedi Aebersold
openaire   +1 more source