GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry [PDF]
International Journal of Molecular Sciences, 2023 Profiling bacterial populations in mixed communities is a common task in microbiology. Sequencing of 16S small subunit ribosomal-RNA (16S rRNA) gene amplicons is a widely accepted and functional approach but relies on amplification primers and cannot quantify isotope incorporation.
Simon Klaes +2 more
exaly +5 more sources
Frontiers in Microbiology, 2019 Tandem mass spectrometry-based proteotyping allows characterizing microorganisms in terms of taxonomy and is becoming an important tool for investigating microbial diversity from several ecosystems.
Karim Hayoun +2 more
exaly +4 more sources
Proteotyping to establish gene origin within reassortant influenza viruses.
PLoS ONE, 2011 The application of a rapid and direct proteotyping approach with which to identify the gene origin of viral antigens in a reassortant influenza strain is demonstrated.
Ji-won Ha +2 more
doaj +5 more sources
Exploring Andean High-Altitude Lake Extremophiles through Advanced Proteotyping [PDF]
Journal of Proteome ResearchQuickly identifying and characterizing isolates from extreme environments is currently challenging while very important to explore the Earth′s biodiversity. As these isolates may, in principle, be distantly related to known species, techniques are needed
Katharina Runzheimer, Jean Armengaud
exaly +8 more sources
Differentiation of Campylobacter fetus subspecies by proteotyping [PDF]
European Journal of Microbiology and Immunology, 2019 Campylobacter fetus is a causative agent of intestinal illness and, occasionally, severe systemic infections and meningitis. C. fetus currently comprises three subspecies: C. fetus subspecies fetus (Cff), C. fetus subspecies venerealis (Cfv), and C. fetus subspecies testudinum (Cft).
Emele, Matthias F +6 more
openaire +7 more sources
Label-Free Multiplex Proteotyping of Microbial Isolates
Analytical Chemistry, 2023 To meet clinical diagnostic needs and for general microbiological screening, it is essential to be able to accurately and rapidly identify any microorganisms from complex microbiota. To gain insight into the individual components of microbiota, culturomics has been proposed as a means to systematically test hundreds of possible cultivation conditions ...
Chabas, Madisson +3 more
core +6 more sources
Mix24X, a Lab-Assembled Reference to Evaluate Interpretation Procedures for Tandem Mass Spectrometry Proteotyping of Complex Samples [PDF]
International Journal of Molecular Sciences, 2023 International audienceCorrect identification of the microorganisms present in a complex sample is a crucial issue. Proteotyping based on tandem mass spectrometry can help establish an inventory of organisms present in a sample.
Beatrice Alpha-Bazin +2 more
exaly +3 more sources
Frontiers in Microbiology, 2019 Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system.
Matthias F. Emele +16 more
doaj +2 more sources
Evaluation of two plasma‐based proteotyping assays against APOE ε4 genotyping in a memory clinic setting: The Gothenburg H70 Clinical Studies [PDF]
Alzheimer's and DementiaINTRODUCTION Apolipoprotein E (APOE) epsilon 4 allele status is associated with an increased risk of Alzheimer's disease and should be determined prior to initiation of anti-amyloid beta antibody treatment, because of increased risk of treatment-related ...
Anna Dittrich +2 more
exaly +3 more sources
Revealing taxonomy, activity, and substrate assimilation in mixed bacterial communities by GroEL-proteotyping-based stable isotope probing [PDF]
iScienceSummary: Protein-based stable isotope probing (protein-SIP) can link microbial taxa to substrate assimilation. Traditionally, protein-SIP requires a sample-specific metagenome-derived database for samples with unknown composition. Here, we describe GroEL-
Simon Klaes +4 more
doaj +2 more sources