Exploring Andean High-Altitude Lake Extremophiles through Advanced Proteotyping [PDF]
Journal of Proteome ResearchQuickly identifying and characterizing isolates from extreme environments is currently challenging while very important to explore the Earth′s biodiversity. As these isolates may, in principle, be distantly related to known species, techniques are needed
Katharina Runzheimer, Jean Armengaud
exaly +15 more sources
GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry [PDF]
International Journal of Molecular Sciences, 2023 Profiling bacterial populations in mixed communities is a common task in microbiology. Sequencing of 16S small subunit ribosomal-RNA (16S rRNA) gene amplicons is a widely accepted and functional approach but relies on amplification primers and cannot quantify isotope incorporation.
Simon Klaes +2 more
exaly +4 more sources
Revealing taxonomy, activity, and substrate assimilation in mixed bacterial communities by GroEL-proteotyping-based stable isotope probing [PDF]
iScienceSummary: Protein-based stable isotope probing (protein-SIP) can link microbial taxa to substrate assimilation. Traditionally, protein-SIP requires a sample-specific metagenome-derived database for samples with unknown composition. Here, we describe GroEL-
Simon Klaes +4 more
doaj +2 more sources
Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes [PDF]
Frontiers in Microbiology, 2019 Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system.
Matthias F. Emele +16 more
doaj +2 more sources
Photo‐Catalyzed Labeling of Amyloid Deposits in Human Tissue to Proteotype Amyloidosis Diseases
AggregateOver 40 amyloidogenic proteins have been identified to cause amyloidosis diseases in clinics. Tissue deposition of amyloid proteins entangled with interacting partners is a characteristic pathological hallmark of amyloidosis diseases.
Huan Feng +10 more
doaj +2 more sources
FluShuffle and FluResort: new algorithms to identify reassorted strains of the influenza virus by mass spectrometry [PDF]
BMC Bioinformatics, 2012 Background Influenza is one of the oldest and deadliest infectious diseases known to man. Reassorted strains of the virus pose the greatest risk to both human and animal health and have been associated with all pandemics of the past century, with the ...
Lun Aaron TL +2 more
doaj +3 more sources
Proteotyping of knockout mouse strains reveals sex- and strain-specific signatures in blood plasma [PDF]
npj Systems Biology and Applications, 2021 We proteotyped blood plasma from 30 mouse knockout strains and corresponding wild-type mice from the International Mouse Phenotyping Consortium. We used targeted proteomics with internal standards to quantify 375 proteins in 218 samples.
Yassene Mohammed +11 more
doaj +4 more sources
Proteotyping to establish gene origin within reassortant influenza viruses.
PLoS ONE, 2011 The application of a rapid and direct proteotyping approach with which to identify the gene origin of viral antigens in a reassortant influenza strain is demonstrated.
Ji-won Ha +2 more
doaj +4 more sources
The astounding exhaustiveness and speed of the Astral mass analyzer for highly complex samples is a quantum leap in the functional analysis of microbiomes [PDF]
MicrobiomeBackground By analyzing the proteins which are the workhorses of biological systems, metaproteomics allows us to list the taxa present in any microbiota, monitor their relative biomass, and characterize the functioning of complex biological systems ...
Thibaut Dumas +6 more
doaj +2 more sources
Induced pluripotent stem cell-derived hepatocytes reveal TCA cycle disruption and the potential basis for triheptanoin treatment for malate dehydrogenase 2 deficiency [PDF]
Molecular Genetics and Metabolism ReportsMitochondrial malate dehydrogenase 2 (MDH2) is crucial to cellular energy generation through direct participation in the tricarboxylic acid (TCA) cycle and the malate aspartate shuttle (MAS).
Déborah Mathis +8 more
doaj +2 more sources