Results 211 to 220 of about 904,217 (264)
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Protoplast fusion and the cell cycle
Plant Cell Reports, 1982Protoplasts isolated from rapidly dividing cell suspension cultures of either Nicotiana sylvestris or tumor derived cultures of Crepis capillaris were fused by PEG or liposome treatments to form homokaryons. Analysis of binucleates by Feulgen microspectrophotometry, and autoradiography, has revealed that whereas fusion products of all cell cycle ...
S E, Ashmore, A R, Gould
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Intra- and Interspecies Virus Transfer in Aspergilli via Protoplast Fusion
Fungal Genetics and Biology, 1998 Intra- and interspecies transfer of dsRNA viruses between black Aspergilli and Aspergillus nidulans strains has been investigated using protoplast fusion. We found interspecies transfer of virus in all combinations of black Aspergillus and A.
Ronnie Hoekstra +2 more
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Development of Protoplast Fusion Products
1986The preceding chapter was devoted to the establishment of protoplast fusion bodies and processes of their early development; growth and differentiation of fusant cell clones and plants are treated in this part of the Volume. Proliferation and organogenesis, segregation and recombination of plastids and mitochondria, as well as loss of chromosomes, are ...
R, Nehls +2 more
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Fusion and transformation of plant protoplasts
1986Publisher Summary Somatic hybridization of plants relies on the availability of efficient and reproducible methods for the induced fusion of protoplasts while maintaining high viability and subsequent growth of the product of fusion. The desired level of fusion depends on the mode of selection operating between the two species being hybridized.
J.B. POWER +4 more
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Journal of Tissue Culture Methods, 1989
This report describes several techniques for plant gene transfer by protoplast fusion. These detailed procedures are adaptable to a wide variety of plant species for the transfer of either the entire or partial genomes of nuclear, mitochondrial, and chloroplast origins between species.
Stephen Gleddie, Wilfred A. Keller
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This report describes several techniques for plant gene transfer by protoplast fusion. These detailed procedures are adaptable to a wide variety of plant species for the transfer of either the entire or partial genomes of nuclear, mitochondrial, and chloroplast origins between species.
Stephen Gleddie, Wilfred A. Keller
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Protoplast Fusion and Protoplast Transformation in Micromonospora Species
1983It is well known that Micromonospora species produce important antibiotics but very few genetic works have been done with them. The new genetic manipulations are more frequently applied in the recent years in the strain improvement programs of antibiotics producing microorganisms.
M. Szigetvári-Gabányi, M. Járai
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Journal of Plant Physiology, 1988
Summary Isolated protoplasts were embedded into gel droplets consisting of calcium nitrate solution solidified with agarose, and induced to fuse by liquid calcium nitrate solution adjusted to high pH values by ammonia. This procedure enabled the observation of the fusion process and the early development of the fusants in a fixed location of the ...
Horst Binding +3 more
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Summary Isolated protoplasts were embedded into gel droplets consisting of calcium nitrate solution solidified with agarose, and induced to fuse by liquid calcium nitrate solution adjusted to high pH values by ammonia. This procedure enabled the observation of the fusion process and the early development of the fusants in a fixed location of the ...
Horst Binding +3 more
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Plant Molecular Biology Reporter, 1984
Carrot protoplasts have been used successfully in many protoplast fusion studies. These studies have demonstrated 1) whether or not the expression of certain resistances is dominant or recessive, 2) the development of a “universla hybridzer,” 3) the use of chemicals to inactivate one parent, 50 the complementation of albino mutants, 5) interspecific ...
J. M. Widholm +2 more
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Carrot protoplasts have been used successfully in many protoplast fusion studies. These studies have demonstrated 1) whether or not the expression of certain resistances is dominant or recessive, 2) the development of a “universla hybridzer,” 3) the use of chemicals to inactivate one parent, 50 the complementation of albino mutants, 5) interspecific ...
J. M. Widholm +2 more
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Protoplast Formation and Fusion
1989Protoplasts can be used in numerous studies, other than for hybridization, including investigations involving isolation of nuclear and mitochondrial DNA, and especially for isolation and separation of relatively intact chromosomes or chromosome-sized sections of DNA.
John F. T. Spencer +2 more
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1983
The yeasts are a group of lower eukaryotic microorganisms showing biological and biochemical diversity. In common usage the term “yeast” is used to describe strains of Saccharomyces cerevisiae that have great commercial value in baking, brewing, and distilling.
Richard F. Freeman, John F. Peberdy
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The yeasts are a group of lower eukaryotic microorganisms showing biological and biochemical diversity. In common usage the term “yeast” is used to describe strains of Saccharomyces cerevisiae that have great commercial value in baking, brewing, and distilling.
Richard F. Freeman, John F. Peberdy
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